ER81 (ETV1) (NM_001163150) Human Untagged Clone

CAT#: SC326983

ETV1 (untagged)-Human ets variant 1 (ETV1) transcript variant 5

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ETV1
• Synonyms: ER81
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001163150, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCTTCAAGATTTAAGTGCAAGTGTCTTCTTTCCACCTTGTTCACAACACAGAACGTTAGCTCAGGTAC
  CTGACAATGATGAGCAGTTTGTACCAGACTATCAGGCTGAAAGTTTGGCTTTTCATGGCCTGCCACTGAA
  AATCAAGAAAGAACCCCACAGTCCATGTTCAGAAATCAGCTCTGCCTGCAGTCAAGAACAGCCCTTTAAA
  TTCAGCTATGGAGAAAAGTGCCTGTACAATGTCAGTGCCTATGATCAGAAGCCACAAGTGGGAATGAGGC
  CCTCCAACCCCCCCACACCATCCAGCACGCCAGTGTCCCCACTGCATCATGCATCTCCAAACTCAACTCA
  TACACCGAAACCTGACCGGGCCTTCCCAGCTCACCTCCCTCCATCGCAGTCCATACCAGATAGCAGCTAC
  CCCATGGACCACAGATTTCGCCGCCAGCTTTCTGAACCCTGTAACTCCTTTCCTCCTTTGCCGACGATGC
  CAAGGGAAGGACGTCCTATGTACCAACGCCAGATGTCTGAGCCAAACATCCCCTTCCCACCACAAGGCTT
  TAAGCAGGAGTACCACGACCCAGTGTATGAACACAACACCATGGTTGGCAGTGCGGCCAGCCAAAGCTTT
  CCCCCTCCTCTGATGATTAAACAGGAACCCAGAGATTTTGCATATGACTCAGAAGTGCCTAGCTGCCACT
  CCATTTATATGAGGCAAGAAGGCTTCCTGGCTCATCCCAGCAGAACAGAAGGCTGTATGTTTGAAAAGGG
  CCCCAGGCAGTTTTATGATGACACCTGTGTTGTCCCAGAAAAATTCGATGGAGACATCAAACAAGAGCCA
  GGAATGTATCGGGAAGGACCCACATACCAACGGCGAGGATCACTTCAGCTCTGGCAGTTTTTGGTAGCTC
  TTCTGGATGACCCTTCAAATTCTCATTTTATTGCCTGGACTGGTCGAGGCATGGAATTTAAACTGATTGA
  GCCTGAAGAGGTGGCCCGACGTTGGGGCATTCAGAAAAACAGGCCAGCTATGAACTATGATAAACTTAGC
  CGTTCACTCCGCTATTACTATGAGAAAGGAATTATGCAAAAGGTGGCTGGAGAGAGATATGTCTACAAGT
  TTGTGTGTGATCCAGAAGCCCTTTTCTCCATGGCCTTTCCAGATAATCAGCGTCCACTGCTGAAGACAGA
  CATGGAACGTCACATCAACGAGGAGGACACAGTGCCTCTTTCTCACTTTGATGAGAGCATGGCCTACATG
  CCGGAAGGGGGCTGCTGCAACCCCCACCCCTACAACGAAGGCTACGTGTATTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001163150
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163150.1, NP_001156622.1
• RefSeq Size: 6303 bp
• RefSeq ORF: 1314 bp
• Locus ID: 2115
• Cytogenetics: 7p21.2
• Protein Families: ES Cell Differentiation/IPS, Transcription Factors
• Gene Summary: 'This gene encodes a member of the ETS (E twenty-six) family of transcription factors. The ETS proteins regulate many target genes that modulate biological processes like cell growth, angiogenesis, migration, proliferation and differentiation. All ETS proteins contain an ETS DNA-binding domain that binds to DNA sequences containing the consensus 5'-CGGA[AT]-3'. The protein encoded by this gene contains a conserved short acidic transactivation domain (TAD) in the N-terminal region, in addition to the ETS DNA-binding domain in the C-terminal region. This gene is involved in chromosomal translocations, which result in multiple fusion proteins including EWS-ETV1 in Ewing sarcoma and at least 10 ETV1 partners (see PMID: 19657377, Table 1) in prostate cancer. In addition to chromosomal rearrangement, this gene is overexpressed in prostate cancer, melanoma and gastrointestinal stromal tumor. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2016]'
  Transcript Variant: This variant (5) represents use of an alternate promoter, 5' UTR, and 5' coding region, compared to variant 1. The resulting isoform (d) has a shorter and distinct N-terminus, compared to isoform a. This isoform lacks major part of the N-terminal TAD. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.