SRP54 (NM_001146282) Human Untagged Clone

CAT#: SC327005

SRP54 (untagged)-Human signal recognition particle 54kDa (SRP54) transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SRP54
• Synonyms: signal recognition particle 54kDa
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001146282, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCACCATTATCAATGAAGAGGTCTGCTATTGATCTTGAAGAGATGGCATCTGGTCTTAACAAAAGAA
  AAATGATTCAGCATGCTGTATTTAAAGAACTTGTGAAGCTTGTAGACCCTGGAGTTAAGGCATGGACACC
  CACTAAAGGAAAACAAAATGTGATTATGTTTGTTGGATTGCAAGGGAGTGGTAAAACAACAACATGTTCA
  AAGCTAGCATATTATTACCAGAGGAAAGGTTGGAAGACCTGTTTAATATGTGCAGACACATTCAGAGCAG
  GGGCTTTTGACCAACTAAAACAGAATGCTACCAAAGCAAGAATTCCATTTTATGGAAGCTATACAGAAAT
  GGATCCTGTCATCATTGCTTCTGAAGGAGTAGAGAAATTTAAAAATGAAAATTTTGAAATTATTATTGTT
  GATACAAGTGGCCGCCACAAACAAGAAGACTCTTTGTTTGAAGAAATGCTTCAAGTTGCTAATGCTATAC
  AACCTGATAACATTGTTTATGTGATGGATGCCTCCATTGGGCAGGCTTGTGAAGCCCAGGCTAAGGCTTT
  TAAAGATAAAGTAGATGTAGCCTCAGTAATAGTGACAAAACTTGATGGCCATGCAAAAGGAGGTGGTGCA
  CTCAGTGCAGTCGCTGCCACAAAAAGTCCGATTATTTTCATTGGTACAGGGGAACATATAGATGACTTTG
  AACCTTTCAAAACACAGCCTTTTATTAGCAAACTTCTTGGTATGGGCGACATTGAAGGACTGATAGATAA
  AGTCAACGAGTTGAAGTTGGATGACAATGAAGCACTTATAGAGAAGTTGAAACATGGTCAGTTTACGTTG
  CGAGACATGTATGAGCAATTTCAAAATATCATGAAAATGGGCCCCTTCAGTCAGATCTTGGGGATGATCC
  CTGGTTTTGGGACAGATTTTATGAGCAAAGGAAATGAACAGGAGTCAATGGCAAGGCTAAAGAAATTAAT
  GACAATAATGGATAGTATGAATGATCAAGAACTAGACAGTACGGATGGTGCCAAAGTTTTTAGTAAACAA
  CCAGGAAGAATCCAAAGAGTAGCAAGAGGATCGGGTGTATCAACAAGAGATGTTCAAGAACTTTTGACAC
  AATATACCAAGTTTGCACAGATGGTAAAAAAGATGGGAGGTATCAAAGGACTTTTCAAAGGTGGCGACAT
  GTCTAAGAATGTGAGCCAGTCACAGATGGCAAAATTGAACCAACAAATGGCCAAAATGATGGATCCTAGG
  GTTCTTCATCACATGGGTGGTATGGCAGGACTTCAGTCAATGATGAGGCAGTTTCAACAGGGTGCTGCTG
  GCAACATGAAAGGCATGATGGGATTCAATAATATGTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001146282
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001146282.1, NP_001139754.1
• RefSeq Size: 2206 bp
• RefSeq ORF: 1368 bp
• Locus ID: 6729
• Cytogenetics: 14q13.2
• Protein Pathways: Protein export
• Gene Summary: ''
  Transcript Variant: This variant (2) lacks a portion of the 5' coding region and uses a distinct translational start codon, compared to variant 1. The resulting isoform (2) has a distinct N-terminus and is shorter than isoform 1.