RASGRP 4 (RASGRP4) (NM_001146206) Human Untagged Clone

CAT#: SC327032

RASGRP4 (untagged)-Human RAS guanyl releasing protein 4 (RASGRP4) transcript variant f

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RASGRP4
• Synonyms: guanyl nucleotide releasing protein 4; RAS guanyl releasing protein 4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001146206, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAACAGAAAAGACAGTAAGAGGAAGTCCCACCAGGAATGCACCGGAAAAATAGGAGGGCGAGGCCGGC
  CCCGCCAAGTGCGCCGCCACAAGACATGCCCCAGCCCTCGGGAAATCAGCAAGGTCATGGCTTCCATGAA
  CCTGGGCCTGCTGAGTGAGGGCGGCTGCAGCGAAGATGAGCTGCTGGAGAAATGCATCCAGTCCTTCGAT
  TCAGCTGGCAGCCTGTGCCACGAGGACCACATGCTCAACATGGTGCTGGCCATGCACAGCTGGGTGCTGC
  CGTCCGCCGACCTGGCTGCCCGCCTGCTGACCTCATACCAGAAGGCCACAGGGGACACCCAGGAGCTGAG
  ACGGCTGCAGATCTGTCACCTGGTCAGGTACTGGCTGATGCGACACCCTGAGGTGATGCACCAGGATCCC
  CAGCTAGAAGAAGTCATAGGTCGTTTCTGGGCCACCGTGGCCCGGGAGGGCAACTCAGCCCAGAGAAGAC
  TGGGAGACTCTTCTGACCTCCTGAGCCCTGGTGGCCCTGGCCCCCCACTCCCAATGAGCAGCCCAGGCCT
  GGGCAAAAAGCGCAAAGTGTCCTTGCTTTTCGACCACTTGGAGACGGGGGAGCTGGCTCAGCACCTCACC
  TACCTGGAGTTCCGGTCCTTCCAGGCTATCACGCTCTCCCTGGACCTCTTCTACACGGAAGACGAGATCT
  ATGAGCTTTCTTATGCCCGGGAGCCGCGTTGTCCCAAGAGCCTGCCACCCTCCCCCTTCAATGCACCTCT
  GGTGGTGGAGTGGGCCCCTGGTGTGACACCCAAGCCGGACAGGGTCACACTGGGTCGGCATGTGGAGCAG
  CTGGTGGAGTCTGTGTTCAAGAATTATGACCCTGAAGGCCGAGGAACAATCTCTCAGGAGGACTTTGAGC
  GACTCTCGGGCAATTTTCCCTTCGCCTGCCATGGGCTTCACCCACCCCCACGCCAGGGGAGAGGATCCTT
  CAGCAGAGAGGAGCTGACAGGGTACCTGCTCCGGGCCAGCGCCATCTGCTCCAAGTTGGGCCTGGCCTTC
  CTGCACACCTTCCATGAGGTCACCTTCCGAAAGCCTACCTTCTGCGACAGCTGCAGTGGCTTCCTCTGGG
  GTGTCACCAAGCAAGGCTACCGCTGTCGGGAGTGCGGGCTGTGTTGCCACAAACACTGCAGAGACCAGGT
  GAAGGTAGAATGTAAGAAGAGGCCAGGGGCCAAGGGCGATGCAGGACCCCCCGGAGCTCCTGTCCCATCC
  ACACCAGCTCCCCATGCCAGCTGTGGCTCCGAGGAAAATCACTCCTACACGCTATCCCTGGAGCCTGAGA
  CTGGGTGCCAGCTTCGCCATGCCTGGACCCAGACTGAATCCCCACACCCTTCCTGGGAAACAGATACGGT
  CCCCTGCCCGGTGATGGACCCACCATCAACTGCATCCTCCAAGCTGGATTCCTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001146206
• ORF Size: 1455 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_001146206.1, NP_001139678.1
• RefSeq Size: 2668
• RefSeq ORF: 1455
• Locus ID: 115727
• Protein Families: Druggable Genome
• Protein Pathways: MAPK signaling pathway
• Gene Summary: The protein encoded by this gene is a member of the Ras guanyl nucleotide-releasing protein (RasGRP) family of Ras guanine nucleotide exchange factors. It contains a Ras exchange motif, a diacylglycerol-binding domain, and two calcium-binding EF hands. This protein was shown to activate H-Ras in a cation-dependent manner in vitro. Expression of this protein in myeloid cell lines was found to be correlated with elevated level of activated RAS protein, and the RAS activation can be greatly enhanced by phorbol ester treatment, which suggested a role of this protein in diacylglycerol regulated cell signaling pathways. Studies of a mast cell leukemia cell line expressing substantial amounts of abnormal transcripts of this gene indicated that this gene may play an important role in the final stages of mast cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2009]
  Transcript Variant: This variant (f) lacks an alternate in-frame segment compared to variant a. The resulting isoform (f) has the same N- and C-termini but is shorter compared to isoform a.