CD39 (ENTPD1) (NM_001164178) Human Untagged Clone

CAT#: SC327061

ENTPD1 (untagged)-Human ectonucleoside triphosphate diphosphohydrolase 1 (ENTPD1) transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ENTPD1
• Synonyms: ATPDase; CD39; NTPDase-1; SPG64
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001164178, the custom clone sequence may differ by one or more nucleotides
  ATGGGGAGGGAAGAACTGTTCTTGACTTTCAGTTTTTCGAGCGGGTTTCAAGAGTCTAAC
  GTGAAGACATTTTGCTCCAAGAATATCCTAGCCATCCTTGGCTTCTCCTCTATCATAGCT
  GTGATAGCTTTGCTTGCTGTGGGGTTGACCCAGAACAAAGCATTGCCAGAAAACGTTAAG
  TATGGGATTGTGCTGGATGCGGGTTCTTCTCACACAAGTTTATACATCTATAAGTGGCCA
  GCAGAAAAGGAGAATGACACAGGCGTGGTGCATCAAGTAGAAGAATGCAGGGTTAAAGGT
  CCTGGAATCTCAAAATTTGTTCAGAAAGTAAATGAAATAGGCATTTACCTGACTGATTGC
  ATGGAAAGAGCTAGGGAAGTGATTCCAAGGTCCCAGCACCAAGAGACACCCGTTTACCTG
  GGAGCCACGGCAGGCATGCGGTTGCTCAGGATGGAAAGTGAAGAGTTGGCAGACAGGGTT
  CTGGATGTGGTGGAGAGGAGCCTCAGCAACTACCCCTTTGACTTCCAGGGTGCCAGGATC
  ATTACTGGCCAAGAGGAAGGTGCCTATGGCTGGATTACTATCAACTATCTGCTGGGCAAA
  TTCAGTCAGAAAACAAGGTGGTTCAGCATAGTCCCATATGAAACCAATAATCAGGAAACC
  TTTGGAGCTTTGGACCTTGGGGGAGCCTCTACACAAGTCACTTTTGTACCCCAAAACCAG
  ACTATCGAGTCCCCAGATAATGCTCTGCAATTTCGCCTCTATGGCAAGGACTACAATGTC
  TACACACATAGCTTCTTGTGCTATGGGAAGGATCAGGCACTCTGGCAGAAACTGGCCAAG
  GACATTCAGGTTGCAAGTAATGAAATTCTCAGGGACCCATGCTTTCATCCTGGATATAAG
  AAGGTAGTGAACGTAAGTGACCTTTACAAGACCCCCTGCACCAAGAGATTTGAGATGACT
  CTTCCATTCCAGCAGTTTGAAATCCAGGGTATTGGAAACTATCAACAATGCCATCAAAGC
  ATCCTGGAGCTCTTCAACACCAGTTACTGCCCTTACTCCCAGTGTGCCTTCAATGGGATT
  TTCTTGCCACCACTCCAGGGGGATTTTGGGGCATTTTCAGCTTTTTACTTTGTGATGAAG
  TTTTTAAACTTGACATCAGAGAAAGTCTCTCAGGAAAAGGTGACTGAGATGATGAAAAAG
  TTCTGTGCTCAGCCTTGGGAGGAGATAAAAACATCTTACGCTGGAGTAAAGGAGAAGTAC
  CTGAGTGAATACTGCTTTTCTGGTACCTACATTCTCTCCCTCCTTCTGCAAGGCTATCAT
  TTCACAGCTGATTCCTGGGAGCACATCCATTTCATTGGCAAGATCCAGGGCAGCGACGCC
  GGCTGGACTTTGGGCTACATGCTGAACCTGACCAACATGATCCCAGCTGAGCAACCATTG
  TCCACACCTCTCTCCCACTCCACCTATGTCTTCCTCATGGTTCTATTCTCCCTGGTCCTT
  TTCACAGTGGCCATCATAGGCTTGCTTATCTTTCACAAGCCTTCATATTTCTGGAAAGAT
  ATGGTA
  
• Restriction Sites: Please inquire
• ACCN: NM_001164178
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164178.1, NP_001157650.1
• RefSeq Size: 12515 bp
• RefSeq ORF: 1569 bp
• Locus ID: 953
• Cytogenetics: 10q24.1
• Protein Families: Transmembrane
• Protein Pathways: Purine metabolism, Pyrimidine metabolism
• Gene Summary: 'The protein encoded by this gene is a plasma membrane protein that hydrolyzes extracellular ATP and ADP to AMP. Inhibition of this protein's activity may confer anticancer benefits. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2015]'
  Transcript Variant: This variant (3) uses an alternate 5' exon that results in a distinct 5' UTR and causes translation initiation at an alternate start codon, compared to variant 1. The encoded isoform (3) has a longer and distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.