eNOS (NOS3) (NM_001160110) Human Untagged Clone

CAT#: SC327138

NOS3 (untagged)-Human nitric oxide synthase 3 (endothelial cell) (NOS3) transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NOS3
• Synonyms: ECNOS; eNOS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001160110, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGCAACTTGAAGAGCGTGGCCCAGGAGCCTGGGCCACCCTGCGGCCTGGGGCTGGGGCTGGGCCTTG
  GGCTGTGCGGCAAGCAGGGCCCAGCCACCCCGGCCCCTGAGCCCAGCCGGGCCCCAGCATCCCTACTCCC
  ACCAGCGCCAGAACACAGCCCCCCGAGCTCCCCGCTAACCCAGCCCCCAGAGGGGCCCAAGTTCCCTCGT
  GTGAAGAACTGGGAGGTGGGGAGCATCACCTATGACACCCTCAGCGCCCAGGCGCAGCAGGATGGGCCCT
  GCACCCCAAGACGCTGCCTGGGCTCCCTGGTATTTCCACGGAAACTACAGGGCCGGCCCTCCCCCGGCCC
  CCCGGCCCCTGAGCAGCTGCTGAGTCAGGCCCGGGACTTCATCAACCAGTACTACAGCTCCATTAAGAGG
  AGCGGCTCCCAGGCCCACGAACAGCGGCTTCAAGAGGTGGAAGCCGAGGTGGCAGCCACAGGCACCTACC
  AGCTTAGGGAGAGCGAGCTGGTGTTCGGGGCTAAGCAGGCCTGGCGCAACGCTCCCCGCTGCGTGGGCCG
  GATCCAGTGGGGGAAGCTGCAGGTGTTCGATGCCCGGGACTGCAGGTCTGCACAGGAAATGTTCACCTAC
  ATCTGCAACCACATCAAGTATGCCACCAACCGGGGCAACCTTCGCTCGGCCATCACAGTGTTCCCGCAGC
  GCTGCCCTGGCCGAGGAGACTTCCGAATCTGGAACAGCCAGCTGGTGCGCTACGCGGGCTACCGGCAGCA
  GGATGGCTCTGTGCGGGGGGACCCAGCCAACGTGGAGATCACCGAGCTCTGCATTCAGCACGGCTGGACC
  CCAGGAAACGGTCGCTTCGACGTGCTGCCCCTGCTGCTGCAGGCCCCAGATGATCCCCCAGAACTCTTCC
  TTCTGCCCCCCGAGCTGGTCCTTGAGGTGCCCCTGGAGCACCCCACGCTGGAGTGGTTTGCAGCCCTGGG
  CCTGCGCTGGTACGCCCTCCCGGCAGTGTCCAACATGCTGCTGGAAATTGGGGGCCTGGAGTTCCCCGCA
  GCCCCCTTCAGTGGCTGGTACATGAGCACTGAGATCGGCACGAGGAACCTGTGTGACCCTCACCGCTACA
  ACATCCTGGAGGATGTGGCTGTCTGCATGGACCTGGATACCCGGACCACCTCGTCCCTGTGGAAAGACAA
  GGCAGCAGTGGAAATCAACGTGGCCGTGCTGCACAGTTACCAGCTAGCCAAAGTCACCATCGTGGACCAC
  CACGCCGCCACGGCCTCTTTCATGAAGCACCTGGAGAATGAGCAGAAGGCCAGGGGGGGCTGCCCTGCAG
  ACTGGGCCTGGATCGTGCCCCCCATCTCGGGCAGCCTCACTCCTGTTTTCCATCAGGAGATGGTCAACTA
  TTTCCTGTCCCCGGCCTTCCGCTACCAGCCAGACCCCTGGAAGGGGAGTGCCGCCAAGGGCACCGGCATC
  ACCAGGAAGAAGACCTTTAAAGAAGTGGCCAACGCCGTGAAGATCTCCGCCTCGCTCATGGGCACGGTGA
  TGGCGAAGCGAGTGAAGGCGACAATCCTGTATGGCTCCGAGACCGGCCGGGCCCAGAGCTACGCACAGCA
  GCTGGGGAGACTCTTCCGGAAGGCTTTTGATCCCCGGGTCCTGTGTATGGATGAGTATGACGTGGTGTCC
  CTCGAACACGAGACGCTGGTGCTGGTGGTAACCAGCACATTTGGGAATGGGGATCCCCCGGAGAATGGAG
  AGAGATGGGGTTTCGCCATGTTGCCCAGGCTGGTCTCTAACTCCTGGGTTCAAGCAATCCACCTGCCTCG
  GCCTCCCAAAGTGCTGCGATTATAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001160110
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001160110.1, NP_001153582.1
• RefSeq Size: 2386 bp
• RefSeq ORF: 1845 bp
• Locus ID: 4846
• Cytogenetics: 7q36.1
• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Arginine and proline metabolism, Calcium signaling pathway, Long-term depression, Metabolic pathways, Pathways in cancer, Small cell lung cancer, VEGF signaling pathway
• Gene Summary: 'Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Oct 2016]'
  Transcript Variant: This variant (3), also known as eNOS13B, uses an alternate 3' exon and poly-adenylation site, compared to variant 1. The resulting isoform (3) has a shorter and distinct C-terminus, compared to isoform 1.