ALS (IGFALS) (NM_001146006) Human Untagged Clone

CAT#: SC327151

IGFALS (untagged)-Human insulin-like growth factor binding protein acid labile subunit (IGFALS) transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IGFALS
• Synonyms: ACLSD; ALS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001146006, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCCTGAGGAAAGCGGGTGACCTCGAGCCTCAGTTCACACCTGAGCGCCGCTTCCGGCTGTGCTGGT
  ATCAGGCTCACTCGGGCAGAGCCCTCCTCGGGCCGCCTCCCCAAGCCTCTCCCCCCGCAGGAGGCCTGGC
  CCTGGCGCTGCTGCTGCTGTCCTGGGTGGCACTGGGCCCCCGCAGCCTGGAGGGAGCAGACCCCGGAACG
  CCGGGGGAAGCCGAGGGCCCAGCGTGCCCGGCCGCCTGTGTCTGCAGCTACGATGACGACGCGGATGAGC
  TCAGCGTCTTCTGCAGCTCCAGGAACCTCACGCGCCTGCCTGATGGAGTCCCGGGCGGCACCCAAGCCCT
  GTGGCTGGACGGCAACAACCTCTCGTCCGTCCCCCCGGCAGCCTTCCAGAACCTCTCCAGCCTGGGCTTC
  CTCAACCTGCAGGGCGGCCAGCTGGGCAGCCTGGAGCCACAGGCGCTGCTGGGCCTAGAGAACCTGTGCC
  ACCTGCACCTGGAGCGGAACCAGCTGCGCAGCCTGGCACTCGGCACGTTTGCACACACGCCCGCGCTGGC
  CTCGCTCGGCCTCAGCAACAACCGTCTGAGCAGGCTGGAGGACGGGCTCTTCGAGGGCCTCGGCAGCCTC
  TGGGACCTCAACCTCGGCTGGAATAGCCTGGCGGTGCTCCCCGATGCGGCGTTCCGCGGCCTGGGCAGCC
  TGCGCGAGCTGGTGCTGGCGGGCAACAGGCTGGCCTACCTGCAGCCCGCGCTCTTCAGCGGCCTGGCCGA
  GCTCCGGGAGCTGGACCTGAGCAGGAACGCGCTGCGGGCCATCAAGGCAAACGTGTTCGTGCAGCTGCCC
  CGGCTCCAGAAACTCTACCTGGACCGCAACCTCATCGCTGCCGTGGCCCCGGGCGCCTTCCTGGGCCTGA
  AGGCGCTGCGATGGCTGGACCTGTCCCACAACCGCGTGGCTGGCCTCCTGGAGGACACGTTCCCCGGTCT
  GCTGGGCCTGCGTGTGCTGCGGCTGTCCCACAACGCCATCGCCAGCCTGCGGCCCCGCACCTTCAAGGAC
  CTGCACTTCCTGGAGGAGCTGCAGCTGGGCCACAACCGCATCCGGCAGCTGGCTGAGCGCAGCTTTGAGG
  GCCTGGGGCAGCTTGAGGTGCTCACGCTAGACCACAACCAGCTCCAGGAGGTCAAGGCGGGCGCTTTCCT
  CGGCCTCACCAACGTGGCGGTCATGAACCTCTCTGGGAACTGTCTCCGGAACCTTCCGGAGCAGGTGTTC
  CGGGGCCTGGGCAAGCTGCACAGCCTGCACCTGGAGGGCAGCTGCCTGGGACGCATCCGCCCGCACACCT
  TCACCGGCCTCTCGGGGCTCCGCCGACTCTTCCTCAAGGACAACGGCCTCGTGGGCATTGAGGAGCAGAG
  CCTGTGGGGGCTGGCGGAGCTGCTGGAGCTCGACCTGACCTCCAACCAGCTCACGCACCTGCCCCACCGC
  CTCTTCCAGGGCCTGGGCAAGCTGGAGTACCTGCTGCTCTCCCGCAACCGCCTGGCAGAGCTGCCGGCGG
  ACGCCCTGGGCCCCCTGCAGCGGGCCTTCTGGCTGGACGTCTCGCACAACCGCCTGGAGGCATTGCCCAA
  CAGCCTCTTGGCACCACTGGGGCGGCTGCGCTACCTCAGCCTCAGGAACAACTCACTGCGGACCTTCACG
  CCGCAGCCCCCGGGCCTGGAGCGCCTGTGGCTGGAGGGTAACCCCTGGGACTGTGGCTGCCCTCTCAAGG
  CGCTGCGGGACTTCGCCCTGCAGAACCCCAGTGCTGTGCCCCGCTTCGTCCAGGCCATCTGTGAGGGGGA
  CGATTGCCAGCCGCCCGCGTACACCTACAACAACATCACCTGTGCCAGCCCGCCCGAGGTCGTGGGGCTC
  GACCTGCGGGACCTCAGCGAGGCCCACTTTGCTCCCTGCTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001146006
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001146006.1, NP_001139478.1
• RefSeq Size: 2282 bp
• RefSeq ORF: 1932 bp
• Locus ID: 3483
• Cytogenetics: 16p13.3
• Protein Families: Druggable Genome, Secreted Protein
• Gene Summary: 'The protein encoded by this gene is a serum protein that binds insulin-like growth factors, increasing their half-life and their vascular localization. Production of the encoded protein, which contains twenty leucine-rich repeats, is stimulated by growth hormone. Defects in this gene are a cause of acid-labile subunit deficiency, which maifests itself in a delayed and slow puberty. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1).