CHFR (NM_001161345) Human Untagged Clone

CAT#: SC327160

CHFR (untagged)-Human checkpoint with forkhead and ring finger domains (CHFR) transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CHFR
• Synonyms: RNF116; RNF196
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001161345, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGCGGCCCGAGGAAGGCAAGCAGTCGCCGCCGCCGCAGCCCTGGGGACGGCTCCTGCGTCTGGGCG
  CGGAGGAGGGCGAGCCGCACGTCCTCCTGAGGAAGCGGGAGTGGACCATCGGGCGGAGACGAGGTTGCGA
  CCTTTCCTTCCCCAGCAATAAACTGGTCTCTGGAGATCACTGTAGAATTGTAGTGGATGAAAAATCAGGT
  CAGGTGACACTGGAAGATACCAGCACCAGTGGAACAGTGATTAACAAGCTGAAGGTTGTTAAGAAGCAGA
  CATGCCCTTTACAGACTGGGGATGTCATCTACTTGGTGTACAGGAAGAATGAACCGGAACACAACGTGGC
  ATACCTCTATGAATCTTTAAGTGAAAAGCAAGGCATGACACAAGAATCCTTTGAAGCTAACAAGGAAAAT
  GTGTTCCATGGGACCAAAGATACCTCAGGTGCAGGTGCAGGGCGAGGGGCCGATCCCCGGGTCCCTCCGT
  CGTCGCCCGCCACTCAGGTGTGCTTTGAGGAACCACAGCCATCAACATCGACGTCAGACCTCTTCCCCAC
  AGCCTCGGCCTCTTCCACGGAGCCTTCTCCTGCAGGGCGAGAGCGTTCCTCCAGTTGTGGGTCTGGGGGT
  GGTGGCATCTCCCCTAAAGGAAGTGGTCCCTCTGTGGCAAGTGATGAAGTCTCCAGCTTTGCCTCAGCTC
  TCCCAGACAGAAAGACTGCGTCCTTTTCGTCGTTGGAACCCCAGGATCAGGAGGATTTGGAGCCCGTGAA
  GAAGAAAATGAGAGGAGATGGGGACCTTGACCTGAACGGGCAGTTGTTGGTCGCACAACCGCGTAGAAAT
  GCCCAAACCGTCCACGAGGACGTCAGAGCAGCGGCTGGGAAGCCAGACAAGATGGAGGAGACGCTGACAT
  GCATCATCTGCCAGGACCTGCTGCACGACTGCGTGAGTTTGCAGCCCTGCATGCACACGTTCTGCGCGGC
  TTGCTACTCGGGCTGGATGGAGCGCTCGTCCCTGTGTCCTACCTGCCGCTGTCCCGTGGAGCGGATCTGT
  AAAAACCACATCCTCAACAACCTCGTGGAAGCATACCTCATCCAGCATCCAGACAAGAGTCGCAGTGAAG
  AAGATGTGCAAAGTATGGATGCCAGGAATAAAATCACTCAAGACATGCTGCAGCCCAAAGTCAGGCGGTC
  TTTTTCTGATGAAGAAGGGAGTTCAGAGGACCTGCTGGAGCTGTCAGACGTTGACAGTGAGTCCTCAGAC
  ATTAGCCAGCCATACGTCGTGTGCCGGCAGTGTCCTGAGTACAGAAGGCAGGCGGCGCAGCCTCCCCACT
  GCCCAGCACCCGAGGGCGAGCCAGGAGCCCCACAGGCCCTGGGGGATGCACCCTCCACGTCCGTCAGCCT
  GACGACAGTCCAGGATTACGTGTGCCCTCTGCAAGGAAGCCACGCCCTGTGCACCTGCTGCTTCCAGCCC
  ATGCCCGACCGGAGAGCGGAGCGCGAGCAGGACCCGCGTGTCGCCCCTCAGCAGTGTGCGGTCTGCCTGC
  AGCCTTTCTGCCACCTGTACTGGGGCTGCACCCGGACCGGCTGCTACGGCTGCCTGGCCCCGTTTTGTGA
  GCTCAACCTGGGTGACAAGTGTCTGGACGGCGTGCTGAACAACAACAGCTACGAGTCAGACATCCTGAAG
  AATTACCTGGCAACCAGAGGTTTGACATGGAAAAACATGTTGACCGAGAGCCTCGTGGCTCTCCAGCGGG
  GAGTGTTTCTGCTGTCTGATTACAGAGTCACGGGAGACACCGTTCTGTGTTACTGCTGTGGCCTGCGCAG
  CTTCCGTGAGCTGACCTATCAGTATCGGCAGAACATTCCTGCTTCCGAGTTGCCAGTGGCCGTAACATCC
  CGTCCTGACTGCTACTGGGGCCGTAACTGCCGCACTCAGGTGAAAGCTCACCACGCCATGAAATTCAATC
  ATATCTGTGAACAGACAAGGTTCAAAAACTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001161345
• ORF Size: 1992 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_001161345.1, NP_001154817.1
• RefSeq Size: 3288
• RefSeq ORF: 1992
• Locus ID: 55743
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes an E3 ubiquitin-protein ligase required for the maintenance of the antephase checkpoint that regulates cell cycle entry into mitosis and, therefore, may play a key role in cell cycle progression and tumorigenesis. The encoded protein has an N-terminal forkhead-associated domain, a central RING-finger domain, and a cysteine-rich C-terminal region. Alternatively spliced transcript variants that encode different protein isoforms have been described. [provided by RefSeq, Mar 2014]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region compared to variant 1. This results in a shorter protein (isoform 2) compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.