PYGM (NM_001164716) Human Untagged Clone

CAT#: SC327207

PYGM (untagged)-Human phosphorylase glycogen muscle (PYGM) transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PYGM
• Synonyms: glycogen phosphorylase; glycogen storage disease type V; McArdle syndrome; muscle (McArdle syndrome, glycogen storage disease type V); myophosphorylase; phosphorylase, glycogen; phosphorylase, glycogen, muscle
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001164716, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCCCGGCCCCTGTCAGACCAAGAGAAAAGAAAGCAAATCAGTGTGCGTGGCCTGGCCGGCGTGGAGA
  ACGTGACTGAGCTGAAAAAGAACTTCAACCGGCACCTGCATTTCACACTCGTAAAGGACCGCAATGTGGC
  CACCCCACGAGACTACTACTTTGCTCTGGCCCATACCGTGCGCGACCACCTCGTGGGGCGCTGGATCCGC
  ACGCAGCAGCACTACTATGAGAAGGACCCCAAGAAGATCTCCGGGGGCTGGCAGATGGAGGAGGCCGATG
  ACTGGCTTCGCTACGGCAACCCCTGGGAGAAGGCCCGGCCCGAGTTCACGCTACCTGTGCACTTCTACGG
  CCATGTGGAGCACACCAGCCAGGGTGCCAAGTGGGTGGACACACAGGTGGTACTGGCCATGCCCTACGAT
  ACGCCCGTGCCTGGCTATCGCAACAATGTTGTCAACACCATGCGCCTCTGGTCTGCCAAGGCTCCCAATG
  ACTTCAACCTCAAGGACTTCAATGTCGGTGGCTACATCCAGGCTGTGTTGGACCGAAACCTGGCGGAGAA
  CATCTCTCGTGTCCTGTACCCCAATGATAATTTCTTCGAAGGGAAGGAGCTGCGGCTGAAGCAGGAGTAT
  TTCGTGGTGGCTGCCACCCTCCAGGACATCATCCGTCGCTTCAAGTCTTCCAAGTTCGGCTGCCGTGATC
  CCGTGCGCACGAACTTCGATGCCTTCCCAGATAAGGTGGCCATCCAGCTCAATGACACCCACCCCTCCCT
  GGCCATCCCCGAGCTGATGAGGATCCTGGTGGACCTGGAACGGATGGACTGGGACAAGGCGTGGGATGTG
  ACAGTGAGGACCTGTGCCTACACCAACCACACGGTGCTGCCCGAGGCCCTGGAGCGCTGGCCGGTGCACC
  TCTTGGAGACGCTGCTGCCGCGGCACCTCCAGATCATCTACGAGATCAACCAGCGCTTCCTCAACCGGGT
  GGCGGCCGCATTCCCAGGGGACGTAGACCGGCTGCGGCGCATGTCGCTGGTGGAGGAGGGCGCAGTGAAG
  CGCATCAACATGGCACACCTGTGCATCGCGGGGTCGCACGCCGTCAACGGCGTGGCGCGCATCCACTCCG
  AGATCCTCAAGAAGACCATCTTCAAAGACTTCTATGAGCTGGAGCCTCATAAGTTCCAGAATAAGACCAA
  CGGCATCACCCCTCGGCGCTGGCTGGTTCTGTGTAACCCCGGGCTGGCAGAGGTCATTGCTGAGCGCATC
  GGGGAGGACTTCATCTCTGACCTGGACCAGCTGCGCAAACTGCTCTCCTTTGTGGATGATGAAGCTTTCA
  TTCGGGATGTGGCCAAAGTGAAGCAGGAAAACAAGTTGAAGTTTGCTGCCTACCTAGAGAGGGAATACAA
  AGTCCACATCAACCCCAACTCACTCTTCGACATCCAGGTGAAGCGGATTCACGAATATAAACGACAGCTC
  CTCAACTGCCTCCATGTCATCACCCTGTACAACCGCATCAAGAGGGAGCCCAATAAGTTTTTTGTGCCTC
  GGACTGTGATGATTGGAGGGAAGGCTGCACCTGGGTACCACATGGCCAAGATGATCATCAGACTCGTCAC
  AGCCATCGGGGATGTGGTCAACCATGACCCGGCAGTGGGTGACCGCCTCCGTGTCATCTTCCTGGAGAAC
  TACCGAGTCTCACTGGCCGAGAAAGTGATCCCAGCTGCAGACCTCTCTGAGCAGATCTCCACTGCGGGCA
  CTGAAGCCTCAGGCACCGGCAACATGAAGTTCATGCTCAACGGGGCTCTGACCATTGGCACCATGGACGG
  GGCCAATGTGGAGATGGCAGAAGAGGCGGGAGAGGAAAACTTCTTCATCTTTGGCATGCGGGTGGAGGAT
  GTGGATAAGCTTGACCAAAGAGGGTACAATGCCCAGGAGTACTACGATCGCATTCCTGAGCTTCGGCAGG
  TCATTGAGCAGCTGAGCAGTGGCTTCTTCTCCCCCAAACAGCCCGACCTGTTCAAGGACATTGTCAATAT
  GCTCATGCACCATGACCGGTTTAAAGTCTTCGCAGATTATGAAGACTACATTAAATGCCAGGAGAAAGTC
  AGCGCCTTGTACAAGAACCCAAGAGAGTGGACGCGGATGGTGATCCGGAACATAGCCACCTCTGGCAAGT
  TCTCCAGTGACCGCACCATTGCCCAGTATGCCCGGGAGATCTGGGGTGTGGAGCCTTCCCGCCAGCGCCT
  GCCAGCCCCGGATGAGGCCATCTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001164716
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164716.1, NP_001158188.1
• RefSeq Size: 3369 bp
• RefSeq ORF: 2265 bp
• Locus ID: 5837
• Cytogenetics: 11q13.1
• Protein Families: Druggable Genome
• Protein Pathways: Insulin signaling pathway, Starch and sucrose metabolism
• Gene Summary: 'This gene encodes a muscle enzyme involved in glycogenolysis. Highly similar enzymes encoded by different genes are found in liver and brain. Mutations in this gene are associated with McArdle disease (myophosphorylase deficiency), a glycogen storage disease of muscle. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Sep 2009]'
  Transcript Variant: This variant (2) lacks two exons uses an alternate in-frame splice site in the 5' coding region compared to variant 1. The resulting protein (isoform 2) is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.