OGDH (NM_001165036) Human Untagged Clone

CAT#: SC327265

OGDH (untagged)-Human oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide) (OGDH) nuclear gene encoding mitochondrial protein transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: OGDH
• Synonyms: AKGDH; E1k; OGDC
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001165036, the custom clone sequence may differ by one or more nucleotides
  ATGTTTCATTTAAGGACTTGTGCTGCTAAGTTGAGGCCATTGACGGCTTCCCAGACTGTT
  AAGACATTTTCACAAAACAGACCAGCAGCAGCTAGGACATTTCAACAGATTCGGTGCTAT
  TCTGCACCTGTTGCTGCTGAGCCCTTTCTCAGTGGGACTAGTTCGAACTATGTGGAGGAG
  ATGTACTGTGCTTGGCTGGAAAACCCCAAAAGTGTACATAAGTCATGGGACATTTTTTTT
  CGCAACACGAATGCCGGAGCCCCACCGGGCACTGCCTACCAGAGTCCCCTTCCCCTGAGC
  CGAGGCTCCCTGGCTGCTGTGGCCCATGCACAGTCCCTGGTAGAAGCACAGCCCAACGTG
  GACAAGCTCGTGGAGGACCACCTGGCAGTGCAGTCGCTCATCAGGGCATATCAGGTCAGG
  GGTCACCACATTGCAAAACTTGATCCTCTCGGAATTAGTTGTGTAAATTTTGATGATGCT
  CCAGTAACTGTTTCTTCAAACGTGGGGTTCTATGGCCTGGATGAGTCTGACCTCGACAAG
  GTCTTCCACTTGCCCACCACCACTTTCATCGGGGGACAGGAATCAGCACTTCCTCTGCGG
  GAGATCATCCGTCGGCTGGAGATGGCCTACTGCCAGCATATTGGGGTGGAGTTCATGTTC
  ATCAATGACCTGGAGCAGTGCCAGTGGATCCGGCAGAAGTTTGAGACCCCTGGGATCATG
  CAGTTCACAAATGAGGAGAAACGGACCCTGCTGGCCAGGCTTGTGCGGTCCACCAGGTTT
  GAGGAGTTCCTACAGCGGAAGTGGTCCTCTGAGAAGCGCTTTGGTCTAGAAGGCTGCGAG
  GTACTGATCCCTGCCCTCAAGACCATCATTGACAAGTCTAGTGAGAATGGCGTGGACTAC
  GTGATCATGGGCATGCCACACAGAGGGCGGCTGAACGTGCTTGCAAATGTCATCAGGAAG
  GAGCTGGAACAGATCTTCTGTCAATTCGATTCAAAGCTGGAGGCAGCTGATGAGGGCTCC
  GGAGATGTGAAGTACCACCTGGGCATGTATCACCGCAGGATCAATCGTGTCACCGACAGG
  AACATTACCTTGTCCTTGGTGGCCAACCCTTCCCACCTTGAGGCCGCTGACCCCGTGGTG
  ATGGGCAAGACCAAAGCCGAACAGTTTTACTGTGGCGACACTGAAGGGAAAAAGGTCATG
  TCCATCCTGTTGCATGGGGATGCTGCATTTGCTGGCCAGGGCATTGTGTACGAGACCTTC
  CACCTCAGCGACCTGCCATCCTACACAACTCATGGCACCGTGCACGTGGTCGTCAACAAC
  CAGATCGGCTTCACCACCGACCCTCGGATGGCCCGCTCCTCCCCCTACCCCACTGACGTG
  GCCCGAGTGGTGAATGCCCCCATTTTCCACGTGAACTCAGATGACCCCGAGGCTGTCATG
  TACGTGTGCAAAGTGGCGGCCGAGTGGAGGAGCACCTTCCACAAGGACGTGGTTGTCGAT
  TTGGTGTGTTACCGGCGCAACGGCCACAACGAGATGGATGAGCCCATGTTCACGCAGCCG
  CTCATGTACAAGCAGATCCGCAAGCAGAAGCCTGTGTTACAGAAGTACGCTGAGCTGCTG
  GTGTCGCAGGGTGTGGTCAACCAGCCTGAGTATGAGGAGGAAATTTCCAAGTATGATAAG
  ATCTGTGAGGAAGCTTTTGCCAGATCTAAAGATGAGAAGATCTTGCACATTAAGCACTGG
  CTGGACTCTCCCTGGCCTGGCTTCTTCACCCTGGACGGGCAGCCCAGGAGCATGTCCTGC
  CCCTCCACGGGTCTGACGGAGGATATTCTGACACACATCGGGAATGTGGCTAGTTCTGTG
  CCTGTGGAAAACTTTACTATTCATGGAGGGCTGAGCCGGATCTTGAAGACTCGTGGGGAA
  ATGGTGAAGAACCGGACTGTGGACTGGGCTCTAGCGGAGTACATGGCGTTTGGCTCGCTC
  CTGAAGGAGGGCATCCACATTCGGCTGAGCGGCCAGGACGTGGAGCGGGGCACATTCAGC
  CACCGCCACCATGTGCTCCATGACCAGAATGTGGACAAGAGAACCTGCATCCCCATGAAC
  CATCTCTGGCCCAATCAGGCCCCCTATACTGTGTGCAACAGCTCACTGTCTGAGTACGGC
  GTGCTGGGCTTTGAGCTGGGCTTCGCCATGGCCAGTCCTAATGCCCTGGTCCTCTGGGAA
  GCCCAATTTGGTGACTTCCACAACACGGCCCAGTGTATCATCGACCAGTTCATCTGCCCG
  GGACAAGCCAAGTGGGTGCGGCAGAATGGCATCGTGTTGCTGCTGCCCCATGGCATGGAG
  GGCATGGGTCCAGAACATTCCTCCGCCCGCCCAGAGCGGTTCTTGCAGATGTGCAACGAT
  GACCCAGATGTCCTGCCAGACCTTAAAGAAGCCAACTTCGACATCAATCAGCTATATGAC
  TGCAATTGGGTTGTTGTCAACTGCTCCACTCCTGGCAACTTCTTCCACGTGCTACGACGC
  CAGATCCTGCTGCCATTCCGGAAGCCGTTAATTATCTTCACCCCCAAATCCCTGTTGCGC
  CACCCCGAGGCCAGATCCAGCTTTGATGAGATGCTTCCAGGAACCCACTTCCAGCGGGTG
  ATCCCAGAAGATGGCCCTGCAGCTCAGAACCCAGAAAATGTCAAAAGGCTTCTCTTCTGC
  ACCGGCAAAGTGTATTATGACCTCACCCGGGAGCGCAAAGCACGCGACATGGTGGGGCAG
  GTGGCCATCACAAGGATTGAGCAGCTGTCGCCATTCCCCTTTGACCTCCTGCTGAAGGAG
  GTGCAGAAGTACCCCAATGCTGAGCTGGCCTGGTGCCAGGAGGAGCACAAGAACCAAGGC
  TACTATGACTACGTGAAGCCAAGACTTCGGACCACCATCAGCCGCGCCAAGCCCGTCTGG
  TATGCCGGCCGGGACCCAGCGGCTGCTCCAGCCACCGGCAACAAGAAGACCCACCTGACG
  GAGCTGCAGCGCCTCCTGGACACGGCCTTCGACCTGGACGTCTTCAAGAACTTCTCG
  
• Restriction Sites: Please inquire
• ACCN: NM_001165036
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001165036.1, NP_001158508.1
• RefSeq Size: 4307 bp
• RefSeq ORF: 3060 bp
• Locus ID: 4967
• Cytogenetics: 7p13
• Protein Families: Druggable Genome
• Protein Pathways: Citrate cycle (TCA cycle), Lysine degradation, Metabolic pathways, Tryptophan metabolism
• Gene Summary: 'This gene encodes one subunit of the 2-oxoglutarate dehydrogenase complex. This complex catalyzes the overall conversion of 2-oxoglutarate (alpha-ketoglutarate) to succinyl-CoA and CO(2) during the Krebs cycle. The protein is located in the mitochondrial matrix and uses thiamine pyrophosphate as a cofactor. A congenital deficiency in 2-oxoglutarate dehydrogenase activity is believed to lead to hypotonia, metabolic acidosis, and hyperlactatemia. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Sep 2009]'
  Transcript Variant: This variant (3) has multiple differences in the presence and absence of exons in the 5' coding region, compared to variant 1. These differences result in a shorter protein (isoform 3), compared to isoform 1.