SLC12A4 (NM_001145964) Human Untagged Clone

CAT#: SC327269

SLC12A4 (untagged)-Human solute carrier family 12 (potassium/chloride transporters) member 4 (SLC12A4) transcript variant 5

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SLC12A4
• Synonyms: CTC-479C5.17; hKCC1; KCC1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001145964, the custom clone sequence may differ by one or more nucleotides
  ATGGGGGACACCCTGAGCCCCGGACATGGCAACCACAGAGAGAGCAGCCCTTTTCTTTCC
  CCCTTGGAGGCTTCCAGAGGAATTGACTACTATGACAGGAACCTGGCACTGTTTGAGGAA
  GAGCTGGACATCCGCCCAAAGGTATCGTCTCTTCTGGGAAAGCTCGTCAGCTACACCAAC
  CTCACCCAGGGCGCCAAAGAGCATGAGGAGGCCGAGAGTGGGGAGGGCACCCGCCGGAGG
  GCAGCCGAGGCACCCAGCATGGGCACCCTCATGGGGGTGTACCTGCCCTGCCTGCAGAAT
  ATCTTTGGGGTTATCCTCTTCCTGCGGCTGACCTGGATGGTGGGCACAGCAGGTGTGCTA
  CAGGCCCTCCTCATCGTGCTTATCTGCTGCTGTTGTACCCTGCTGACGGCCATCTCCATG
  AGTGCCATCGCCACCAACGGTGTGGTTCCAGCTGGGGGCTCCTATTTCATGATCTCTCGT
  TCACTGGGGCCAGAATTTGGAGGTGCTGTGGGCCTGTGCTTCTACCTGGGAACAACATTC
  GCAGCAGCCATGTACATCCTGGGGGCCATCGAGATCTTGCTGACCTACATTGCCCCACCA
  GCTGCCATTTTTTACCCATCGGGTGCTCATGACACGTCGAATGCCACTTTGAACAATATG
  CGTGTGTATGGGACCATTTTCCTGACCTTCATGACCCTGGTGGTGTTTGTGGGGGTCAAG
  TATGTGAACAAATTTGCCTCGCTCTTCCTGGCCTGTGTGATCATCTCCATCCTCTCCATC
  TATGCTGGGGGCATAAAGTCTATATTTGACCCTCCCGTGTTTCCGGTATGCATGCTGGGC
  AACAGGACCCTGTCCCGGGACCAGTTTGACATCTGTGCCAAGACAGCTGTAGTGGACAAT
  GAGACAGTGGCCACCCAGCTATGGAGTTTCTTCTGCCACAGCCCCAACCTTACGACCGAC
  TCCTGTGACCCCTACTTCATGCTCAACAATGTGACCGAGATCCCTGGCATCCCCGGGGCA
  GCTGCTGGTGTGCTCCAGGAAAACCTGTGGAGCGCCTACCTGGAGAAGGGTGACATCGTG
  GAGAAGCATGGGCTGCCCTCCGCAGATGCCCCGAGCCTGAAGGAGAGCCTGCCTCTGTAC
  GTGGTCGCTGACATCGCCACATCCTTCACCGTGCTGGTCGGCATCTTCTTCCCTTCTGTA
  ACAGGCATCATGGCTGGCTCAAACCGCTCTGGGGACCTTCGTGACGCCCAGAAGTCTATC
  CCTGTGGGGACCATTCTGGCCATCATTACAACTTCCCTCGTGTACTTCAGCAGTGTGGTT
  CTCTTTGGTGCCTGCATTGAGGGTGTGGTTCTCCGGGACAAGTATGGCGATGGTGTCAGC
  AGGAACTTGGTGGTGGGCACACTGGCCTGGCCTTCACCCTGGGTCATCGTCATCGGCTCC
  TTCTTTTCAACGTGTGGCGCTGGCCTCCAGAGCCTCACAGGGGCACCACGCCTATTGCAG
  GCCATTGCCAAGGACAACATCATCCCCTTCCTCCGGGTGTTTGGCCACGGGAAGGTGAAT
  GGTGAACCCACATGGGCACTCCTCCTGACGGCACTCATCGCCGAGCTGGGCATCCTCATC
  GCCTCCCTCGACATGGTGGCCCCCATCTTATCCATGTTCTTTCTGATGTGCTACCTGTTC
  GTGAACCTCGCCTGTGCGGTGCAGACACTCCTGAGGACCCCCAACTGGCGGCCCCGGTTC
  AAGTACTATCACTGGGCGCTGTCCTTCCTGGGCATGAGTCTCTGCCTGGCCCTTATGTTT
  GTCTCCTCCTGGTACTATGCCCTGGTGGCCATGCTCATCGCCGGCATGATCTACAAATAC
  ATCGAGTACCAAGGGGCTGAGAAGGAGTGGGGTGACGGGATCCGAGGCCTGTCCCTGAGC
  GCTGCCCGCTACGCGCTGTTGCGGCTGGAGGAGGGGCCTCCTCACACCAAGAACTGGCGG
  CCGCAGCTGCTGGTGCTGCTGAAGCTGGACGAGGACCTCCACGTGAAGTACCCGCGGCTC
  CTCACCTTCGCCTCCCAGCTCAAGGCTGGCAAGGGCCTGACCATTGTTGGTTCTGTCATC
  CAGGGGAGCTTCTTGGAGAGCTATGGCGAGGCTCAGGCCGCCGAGCAGACCATCAAGAAC
  ATGATGGAAATTGAGAAGGTGAAGGGCTTCTGCCAGGTGGTGGTGGCCAGCAAGGTGCGG
  GAGGGGCTGGCCCACCTCATCCAGTCCTGTGGCCTGGGAGGCATGCGGCATAACTCCGTG
  GTGCTGGGCTGGCCCTACGGCTGGCGACAGAGCGAGGACCCCCGTGCCTGGAAGACCTTC
  ATTGACACCGTGCGCTGCACTACGGCTGCCCACCTGGCCCTGCTCGTGCCCAAGAACATC
  GCCTTCTACCCCAGCAACCACGAGCGCTACCTGGAGGGCCACATAGACGTGTGGTGGATC
  GTGCACGATGGTGGCATGCTCATGCTTCTGCCCTTCCTGCTGCGCCAGCATAAGGTCTGG
  AGGAAGTGCCGGATGCGCATCTTCACAGTGGCCCAGATGGATGACAACAGCATCCAGATG
  AAGAAGGACCTGGCTGTCTTTCTGTACCATCTGCGCCTTGAGGCCGAGGTGGAGGTGGTG
  GAGATGCATAACAGTGACATCTCTGCATACACCTACGAGCGGACGCTGATGATGGAGCAG
  CGGTCGCAGATGCTGCGGCAGATGAGACTGACCAAGACTGAGCGGGAGCGAGAAGCCCAG
  CTGGTCAAGGATCGGCACTCGGCCCTGCGGCTGGAGAGCCTGTACTCGGACGAGGAAGAT
  GAGTCTGCAGTGGGGGCTGACAAGATCCAGATGACGTGGACCAGGGACAAGTACATGACT
  GAGACCTGGGACCCCAGCCATGCCCCTGACAATTTCCGGGAGCTGGTGCACATTAAGCCG
  GACCAATCCAATGTGCGGCGCATGCACACTGCTGTGAAGCTCAATGAAGTCATTGTCACG
  CGCTCCCACGACGCCCGCCTGGTTCTCCTAAACATGCCTGGCCCACCCAGGAACAGTGAG
  GGCGACGAGAACTACATGGAGTTCCTCGAGGTGCTGACCGAGGGCCTTGAGCGGGTGCTG
  TTGGTGCGCGGTGGTGGCCGTGAAGTCATCACCATCTACTCC
  
• Restriction Sites: Please inquire
• ACCN: NM_001145964
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001145964.1, NP_001139436.1
• RefSeq Size: 4643 bp
• RefSeq ORF: 3165 bp
• Locus ID: 6560
• Cytogenetics: 16q22.1
• Protein Families: Transmembrane
• Gene Summary: 'This gene encodes a member of the SLC12A transporter family. The encoded protein mediates the coupled movement of potassium and chloride ions across the plasma membrane. This gene is expressed ubiquitously. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jan 2013]'
  Transcript Variant: This variant (5) contains an alternate 5' exon which includes a translational start codon, compared to variant 1. The encoded isoform (e) has a distinct N-terminus and is shorter than isoform a.