SLC12A4 (NM_001145963) Human Untagged Clone

CAT#: SC327272

SLC12A4 (untagged)-Human solute carrier family 12 (potassium/chloride transporters) member 4 (SLC12A4) transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SLC12A4
• Synonyms: CTC-479C5.17; hKCC1; KCC1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001145963, the custom clone sequence may differ by one or more nucleotides
  ATGGCAGCCGAAGGTGCTGTGTGCGGCTTCGTCTATCTGGAGGGCACTGCCTGGGCGGTC
  CCTGAGGACACCGAACCCTTGGCATCCTGCACATTGGGACATGGCAACCACAGAGAGAGC
  AGCCCTTTTCTTTCCCCCTTGGAGGCTTCCAGAGGAATTGACTACTATGACAGGAACCTG
  GCACTGTTTGAGGAAGAGCTGGACATCCGCCCAAAGGTATCGTCTCTTCTGGGAAAGCTC
  GTCAGCTACACCAACCTCACCCAGGGCGCCAAAGAGCATGAGGAGGCCGAGAGTGGGGAG
  GGCACCCGCCGGAGGGCAGCCGAGGCACCCAGCATGGGCACCCTCATGGGGGTGTACCTG
  CCCTGCCTGCAGAATATCTTTGGGGTTATCCTCTTCCTGCGGCTGACCTGGATGGTGGGC
  ACAGCAGGTGTGCTACAGGCCCTCCTCATCGTGCTTATCTGCTGCTGTTGTACCCTGCTG
  ACGGCCATCTCCATGAGTGCCATCGCCACCAACGGTGTGGTTCCAGCTGGGGGCTCCTAT
  TTCATGATCTCTCGTTCACTGGGGCCAGAATTTGGAGGTGCTGTGGGCCTGTGCTTCTAC
  CTGGGAACAACATTCGCAGCAGCCATGTACATCCTGGGGGCCATCGAGATCTTGCTGACC
  TACATTGCCCCACCAGCTGCCATTTTTTACCCATCGGGTGCTCATGACACGTCGAATGCC
  ACTTTGAACAATATGCGTGTGTATGGGACCATTTTCCTGACCTTCATGACCCTGGTGGTG
  TTTGTGGGGGTCAAGTATGTGAACAAATTTGCCTCGCTCTTCCTGGCCTGTGTGATCATC
  TCCATCCTCTCCATCTATGCTGGGGGCATAAAGTCTATATTTGACCCTCCCGTGTTTCCG
  GTATGCATGCTGGGCAACAGGACCCTGTCCCGGGACCAGTTTGACATCTGTGCCAAGACA
  GCTGTAGTGGACAATGAGACAGTGGCCACCCAGCTATGGAGTTTCTTCTGCCACAGCCCC
  AACCTTACGACCGACTCCTGTGACCCCTACTTCATGCTCAACAATGTGACCGAGATCCCT
  GGCATCCCCGGGGCAGCTGCTGGTGTGCTCCAGGAAAACCTGTGGAGCGCCTACCTGGAG
  AAGGGTGACATCGTGGAGAAGCATGGGCTGCCCTCCGCAGATGCCCCGAGCCTGAAGGAG
  AGCCTGCCTCTGTACGTGGTCGCTGACATCGCCACATCCTTCACCGTGCTGGTCGGCATC
  TTCTTCCCTTCTGTAACAGGCATCATGGCTGGCTCAAACCGCTCTGGGGACCTTCGTGAC
  GCCCAGAAGTCTATCCCTGTGGGGACCATTCTGGCCATCATTACAACTTCCCTCGTGTAC
  TTCAGCAGTGTGGTTCTCTTTGGTGCCTGCATTGAGGGTGTGGTTCTCCGGGACAAGTAT
  GGCGATGGTGTCAGCAGGAACTTGGTGGTGGGCACACTGGCCTGGCCTTCACCCTGGGTC
  ATCGTCATCGGCTCCTTCTTTTCAACGTGTGGCGCTGGCCTCCAGAGCCTCACAGGGGCA
  CCACGCCTATTGCAGGCCATTGCCAAGGACAACATCATCCCCTTCCTCCGGGTGTTTGGC
  CACGGGAAGGTGAATGGTGAACCCACATGGGCACTCCTCCTGACGGCACTCATCGCCGAG
  CTGGGCATCCTCATCGCCTCCCTCGACATGGTGGCCCCCATCTTATCCATGTTCTTTCTG
  ATGTGCTACCTGTTCGTGAACCTCGCCTGTGCGGTGCAGACACTCCTGAGGACCCCCAAC
  TGGCGGCCCCGGTTCAAGTACTATCACTGGGCGCTGTCCTTCCTGGGCATGAGTCTCTGC
  CTGGCCCTTATGTTTGTCTCCTCCTGGTACTATGCCCTGGTGGCCATGCTCATCGCCGGC
  ATGATCTACAAATACATCGAGTACCAAGGGGCTGAGAAGGAGTGGGGTGACGGGATCCGA
  GGCCTGTCCCTGAGCGCTGCCCGCTACGCGCTGTTGCGGCTGGAGGAGGGGCCTCCTCAC
  ACCAAGAACTGGCGGCCGCAGCTGCTGGTGCTGCTGAAGCTGGACGAGGACCTCCACGTG
  AAGTACCCGCGGCTCCTCACCTTCGCCTCCCAGCTCAAGGCTGGCAAGGGCCTGACCATT
  GTTGGTTCTGTCATCCAGGGGAGCTTCTTGGAGAGCTATGGCGAGGCTCAGGCCGCCGAG
  CAGACCATCAAGAACATGATGGAAATTGAGAAGGTGAAGGGCTTCTGCCAGGTGGTGGTG
  GCCAGCAAGGTGCGGGAGGGGCTGGCCCACCTCATCCAGTCCTGTGGCCTGGGAGGCATG
  CGGCATAACTCCGTGGTGCTGGGCTGGCCCTACGGCTGGCGACAGAGCGAGGACCCCCGT
  GCCTGGAAGACCTTCATTGACACCGTGCGCTGCACTACGGCTGCCCACCTGGCCCTGCTC
  GTGCCCAAGAACATCGCCTTCTACCCCAGCAACCACGAGCGCTACCTGGAGGGCCACATA
  GACGTGTGGTGGATCGTGCACGATGGTGGCATGCTCATGCTTCTGCCCTTCCTGCTGCGC
  CAGCATAAGGTCTGGAGGAAGTGCCGGATGCGCATCTTCACAGTGGCCCAGATGGATGAC
  AACAGCATCCAGATGAAGAAGGACCTGGCTGTCTTTCTGTACCATCTGCGCCTTGAGGCC
  GAGGTGGAGGTGGTGGAGATGCATAACAGTGACATCTCTGCATACACCTACGAGCGGACG
  CTGATGATGGAGCAGCGGTCGCAGATGCTGCGGCAGATGAGACTGACCAAGACTGAGCGG
  GAGCGAGAAGCCCAGCTGGTCAAGGATCGGCACTCGGCCCTGCGGCTGGAGAGCCTGTAC
  TCGGACGAGGAAGATGAGTCTGCAGTGGGGGCTGACAAGATCCAGATGACGTGGACCAGG
  GACAAGTACATGACTGAGACCTGGGACCCCAGCCATGCCCCTGACAATTTCCGGGAGCTG
  GTGCACATTAAGCCGGACCAATCCAATGTGCGGCGCATGCACACTGCTGTGAAGCTCAAT
  GAAGTCATTGTCACGCGCTCCCACGACGCCCGCCTGGTTCTCCTAAACATGCCTGGCCCA
  CCCAGGAACAGTGAGGGCGACGAGAACTACATGGAGTTCCTCGAGGTGCTGACCGAGGGC
  CTTGAGCGGGTGCTGTTGGTGCGCGGTGGTGGCCGTGAAGTCATCACCATCTACTCC
  
• Restriction Sites: Please inquire
• ACCN: NM_001145963
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001145963.1, NP_001139435.1
• RefSeq Size: 4652 bp
• RefSeq ORF: 3240 bp
• Locus ID: 6560
• Cytogenetics: 16q22.1
• Protein Families: Transmembrane
• Gene Summary: 'This gene encodes a member of the SLC12A transporter family. The encoded protein mediates the coupled movement of potassium and chloride ions across the plasma membrane. This gene is expressed ubiquitously. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jan 2013]'
  Transcript Variant: This variant (4) contains an alternate 5' exon which includes a translational start codon, compared to variant 1. The encoded isoform (d) has a distinct N-terminus and is shorter than isoform a.