Phospholipase C epsilon 1 (PLCE1) (NM_001165979) Human Untagged Clone

CAT#: SC327342

PLCE1 (untagged)-Human phospholipase C epsilon 1 (PLCE1) transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PLCE1
• Synonyms: NPHS3; PLCE; PPLC
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001165979, the custom clone sequence may differ by one or more nucleotides
  ATGGTTTCAGAAGGAAGTGCAGCAGGAAGAGACTTTGCAGGAATGGAAGAGGTGAGGCAG
  CTCCACGTGAGATTCTGCAAAGGGATTAAGATTTGGCACCAGGCTTGGTTTCTGTGCAGC
  CTCTTAGGCAGAGAGCCCCAGGAGAGGGAAGCAGGCTGTCAGCTGTGGCTTTGCACGCTC
  TCAGCTGTCTTGAAGGTTGGTTGGCTCTTCCCGCTCTCTGAGGTACCCAATTTTACCTTG
  TTAAAGGATGGATGTGGATGCTGGAGGCTTAAAGAAGACCAGATCTACAATGCAGTGAGA
  AGAGAAGAAACAGAAAATACAGTTGGATCTCTACTCCATTTCCTCACCAAGCTCCCAGCC
  TCCGAGACAGCCCATGGAAGGATAAGCGTTGGTCCATGCTTAAAGCAATGTGTCCGAGAC
  ACTGTATGTGAGTATCGCGCCACCCTCCAAAGGACTTCAATATCGCAGTACATCACCGGT
  TCTCTCCTAGAAGCAACCACGTCTTTGGGAGCAAGAAGTGGCCTTCTCAGTACTTTTGGA
  GGATCCACTGGACGAATGATGCTGAAAGAACGCCAGCCAGGCCCCTCTGTGGCCAATTCC
  AATGCCCTCCCTTCAAGTTCAGCTGGGATCAGCAAGGAGCTGATCGATCTGCAGCCTCTC
  ATCCAGTTCCCAGAGGAAGTCGCCAGCATCCTGATGGAGCAAGAGCAGACTATTTACCGC
  AGGGTCTTGCCAGTCGACTACCTTTGCTTCTTAACACGGGACTTGGGCACTCCTGAATGC
  CAGAGCTCCTTGCCCTGCCTCAAAGCATCCATCTCAGCGTCGATTCTTACCACTCAGAAT
  GGAGAGCACAATGCCCTTGAAGATCTGGTGATGAGGTTTAATGAGGTGAGCTCCTGGGTG
  ACATGGCTGATCCTCACGGCAGGCTCCATGGAGGAGAAGCGAGAAGTCTTTTCATATTTG
  GTGCATGTGGCCAAATGCTGCTGGAACATGGGCAACTACAACGCTGTCATGGAGTTCTTG
  GCTGGCCTCAGGTCAAGAAAAGTTTTAAAAATGTGGCAGTTCATGGACCAGTCTGATATT
  GAGACCATGAGGAGCCTGAAGGATGCTATGGCCCAGCATGAGTCCTCTTGTGAGTACAGA
  AAGGTGGTGACACGTGCCCTGCACATCCCTGGCTGTAAGGTGGTTCCATTCTGTGGGGTG
  TTTCTGAAGGAGCTCTGTGAAGTGCTTGACGGCGCCTCCGGTCTCATGAAGCTTTGCCCG
  CGGTACAATTCCCAAGAAGAAACTTTAGAGTTTGTAGCAGATTACAGTGGACAAGATAAT
  TTCTTACAACGAGTGGGACAAAATGGCTTAAAGAATTCGGAGAAGGAGTCCACTGTCAAC
  AGCATCTTTCAGGTCATCCGGAGCTGCAATCGAAGTCTGGAGACAGACGAGGAGGACAGC
  CCCAGTGAAGGAAACAGCTCCAGGAAAAGCTCCTTGAAGGATAAAAGCCGATGGCAGTTT
  ATAATTGGAGATTTGTTGGATTCAGACAATGACATCTTTGAGCAATCCAAAGAATACGAC
  TCTCATGGTTCAGAGGACTCACAGAAGGCCTTCGACCATGGGACGGAGCTCATCCCTTGG
  TACGTGCTGTCCATCCAAGCCGATGTGCACCAGTTCCTGCTGCAGGGGGCCACGGTCATC
  CACTACGACCAGGACACACACCTCTCTGCCCGCTGCTTCCTCCAGCTTCAGCCCGACAAT
  AGCACCTTGACCTGGGTAAAGCCCACAACTGCCTCCCCAGCCAGCAGTAAAGCAAAACTT
  GGTGTACTTAATAACACAGCTGAGCCTGGAAAATTCCCACTACTGGGTAATGCTGGATTA
  AGTAGCCTGACGGAAGGGGTCTTGGATCTTTTTGCAGTGAAGGCTGTATACATGGGCCAC
  CCTGGCATTGATATACACACTGTGTGTGTTCAGAACAAACTGGGTAGCATGTTCCTGTCA
  GAGACTGGTGTGACATTGCTCTATGGGCTTCAGACCACAGACAACAGATTATTGCACTTC
  GTGGCACCAAAGCACACAGCTAAAATGCTCTTCAGCGGATTATTGGAACTCACTAGAGCT
  GTGAGAAAGATGAGGAAATTCCCTGACCAAAGACAGCAGTGGCTGCGGAAACAGTACGTC
  AGCCTTTATCAGGAGGATGGACGGTATGAAGGCCCAACTTTGGCTCACGCTGTGGAGTTG
  TTTGGTGGCAGACGGTGGAGTGCTCGAAACCCCAGCCCCGGAACATCAGCAAAGAATGCT
  GAGAAGCCCAATATGCAGAGAAACAATACCCTGGGCATAAGCACTACCAAGAAAAAGAAG
  AAAATCCTCATGAGGGGTGAGAGTGGAGAGGTAACTGACGATGAGATGGCAACCCGAAAG
  GCCAAGATGCACAAAGAGTGTCGAAGCCGGAGTGGTTCTGATCCTCAAGACATTAATGAA
  CAAGAAGAATCAGAGGTGAATGCCATCGCTAACCCTCCAAACCCCCTCCCTTCCAGAAGA
  GCCCACTCTTTGACCACAGCTGGGTCCCCCAACTTGGCTGCCGGGACGTCATCTCCCATC
  AGGCCAGTGTCCTCCCCTGTGCTGTCTTCTTCAAACAAGAGCCCATCCAGTGCTTGGAGC
  AGTAGTAGCTGGCACGGGCGGATCAAAGGCGGCATGAAGGGATTTCAGAGCTTCATGGTT
  TCAGATAGCAACATGAGTTTTGTTGAATTTGTTGAGCTGTTCAAATCATTCAGTGTCAGG
  AGCCGCAAGGACCTGAAGGATCTGTTTGATGTCTATGCAGTGCCCTGCAACCGATCTGGC
  TCCGAGTCAGCCCCACTCTACACCAACCTGACAATTGATGAAAACACCAGCGATCTTCAG
  CCTGACCTAGATCTGTTGACCAGAAATGTCTCGGATTTGGGGTTGTTCATTAAGAGTAAA
  CAGCAGCTATCGGACAACCAGAGGCAGATATCTGATGCCATTGCTGCTGCAAGCATTGTG
  ACAAATGGCACTGGGATTGAGAGCACATCTCTGGGCATTTTTGGGGTGGGCATACTTCAG
  CTCAACGATTTCCTCGTGAATTGCCAAGGAGAACACTGCACTTATGATGAAATCCTCAGC
  ATCATCCAGAAGTTCGAGCCTAGCATCAGTATGTGTCATCAGGGACTAATGTCATTTGAA
  GGGTTTGCCAGGTTTCTGATGGATAAAGAAAATTTTGCCTCAAAAAATGATGAGTCACAG
  GAGAACATTAAAGAACTGCAGCTACCCCTCTCATACTATTACATCGAATCTTCGCACAAT
  ACCTACCTCACGGGCCATCAGCTCAAAGGAGAATCCTCGGTAGAACTCTACAGCCAGGTC
  CTTTTGCAAGGCTGTCGAAGTGTAGAATTGGACTGCTGGGACGGAGACGATGGGATGCCC
  ATCATTTATCATGGACATACGCTGACAACCAAGATCCCCTTCAAGGAAGTGGTTGAAGCC
  ATTGATCGCAGTGCCTTCATCAACTCTGACCTGCCAATCATCATATCGATTGAGAACCAC
  TGTTCATTGCCTCAGCAACGAAAAATGGCAGAAATTTTCAAGACTGTGTTTGGAGAAAAG
  CTGGTGACTAAATTCTTATTTGAGACTGATTTCTCAGATGATCCAATGCTTCCTTCACCT
  GACCAACTCAGAAAGAAAGTTCTTCTTAAAAACAAGAAGCTAAAAGCCCATCAGACGCCA
  GTGGATATCTTAAAGCAAAAGGCTCATCAGTTAGCATCTATGCAAGTGCAGGCTTATAAT
  GGTGGGAATGCCAACCCCCGACCTGCCAATAATGAGGAAGAGGAAGATGAGGAGGACGAA
  TATGATTATGACTATGAATCCCTTTCTGATGACAACATTCTGGAAGACAGACCTGAAAAT
  AAATCATGTAATGACAAGCTTCAGTTTGAATATAATGAAGAAATCCCAAAGAGGATAAAG
  AAAGCAGATAACTCTGCTTGCAACAAAGGAAAGGTTTATGATATGGAACTGGGAGAAGAA
  TTTTATCTTGATCAGAATAAAAAGGAAAGCAGACAGATTGCACCAGAGCTTTCTGACCTT
  GTAATCTATTGTCAAGCAGTAAAATTTCCAGGACTGTCAACTCTAAATGCATCTGGCTCT
  AGCAGAGGAAAAGAAAGGAAAAGCAGGAAGTCCATTTTTGGCAACAATCCGGGCAGAATG
  AGCCCAGGGGAGACAGCATCATTTAACAAAACATCTGGAAAAAGTTCCTGTGAAGGCATT
  CGACAGACCTGGGAGGAATCTTCTTCCCCTCTCAACCCAACCACGTCCCTCAGTGCTATC
  ATTAGAACTCCCAAATGTTATCATATCTCGTCGCTGAATGAAAATGCCGCCAAACGTCTG
  TGTCGCAGGTATTCTCAGAAACTGACCCAGCACACCGCCTGTCAGCTGCTGAGAACTTAC
  CCTGCTGCCACCCGCATCGACTCTTCCAACCCGAACCCCCTCATGTTCTGGCTCCATGGG
  ATACAGCTTGTGGCACTCAACTACCAGACTGATGATCTCCCTTTACATTTAAATGCTGCA
  ATGTTTGAGGCAAATGGTGGTTGTGGTTATGTATTGAAACCTCCAGTTCTGTGGGACAAG
  AACTGCCCCATGTATCAGAAGTTTTCTCCACTAGAAAGAGATCTGGACAGCATGGATCCT
  GCAGTCTATTCTTTAACTATTGTCTCTGGTCAGAATGTGTGCCCCAGTAATAGCATGGGA
  AGCCCGTGCATTGAAGTCGACGTCCTGGGCATGCCTCTGGACAGCTGCCATTTCCGCACA
  AAGCCCATCCATCGAAACACCCTGAACCCCATGTGGAACGAGCAGTTTCTGTTCCACGTT
  CACTTCGAAGATCTTGTATTTCTTCGTTTTGCAGTTGTGGAAAACAATAGTTCAGCGGTA
  ACTGCTCAGAGAATCATTCCACTGAAAGCTTTAAAACGAGGATATCGACATCTTCAGCTG
  CGAAACCTTCACAATGAAGTCTTGGAGATTTCTAGTTTATTCATTAACAGCAGAAGGATG
  GAAGAAAATTCCTCTGGCAATACCATGTCAGCCTCTTCGATGTTTAATACAGAAGAAAGA
  AAATGTTTGCAGACTCACAGAGTCACGGTGCATGGGGTCCCAGGGCCAGAGCCCTTTACC
  GTTTTCACTATTAATGGAGGCACCAAGGCAAAGCAGCTTCTGCAGCAAATTCTGACAAAT
  GAACAAGACATCAAACCTGTTACCACAGACTATTTTTTGATGGAAGAAAAATATTTTATA
  TCTAAAGAAAAGAATGAATGTAGGAAACAACCATTCCAGAGAGCCATTGGTCCAGAAGAG
  GAGATCATGCAAATTTTAAGCAGCTGGTTTCCAGAAGAGGGATACATGGGCAGGATTGTC
  TTAAAAACCCAGCAGGAAAACCTAGAAGAGAAAAACATTGTTCAAGATGACAAAGAGGTG
  ATCTTGAGCTCAGAGGAGGAGAGTTTCTTTGTCCAAGTGCATGATGTTTCTCCAGAGCAA
  CCTCGAACAGTCATCAAAGCACCCCGCGTCAGCACTGCACAGGATGTCATTCAGCAGACC
  TTATGCAAAGCCAAATATTCCTACAGCATCCTGAGCAACCCCAATCCAAGCGACTATGTG
  CTTTTGGAAGAGGTGGTGAAAGACACTACCAACAAGAAGACTACCACACCAAAGTCCTCT
  CAGCGGGTCCTTCTGGATCAGGAGTGTGTGTTTCAAGCCCAAAGCAAGTGGAAAGGTGCA
  GGAAAATTCATCCTTAAGCTAAAGGAGCAGGTGCAGGCATCTCGAGAAGATAAAAAGAAA
  GGCATTTCTTTCGCAAGTGAACTCAAGAAGCTCACCAAGTCAACTAAACAGCCCCGAGGA
  CTTACATCACCTTCTCAGCTCTTGACCTCAGAAAGTATCCAAACCAAGGAGGAGAAACCT
  GTGGGTGGCTTGTCCTCCAGTGACACAATGGATTACCGACAG
  
• Restriction Sites: Please inquire
• ACCN: NM_001165979
• ORF Size: 5985 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_001165979.1, NP_001159451.1
• RefSeq Size: 6456
• RefSeq ORF: 5985
• Locus ID: 51196
• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway, Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• Gene Summary: This gene encodes a phospholipase enzyme that catalyzes the hydrolysis of phosphatidylinositol-4,5-bisphosphate to generate two second messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). These second messengers subsequently regulate various processes affecting cell growth, differentiation, and gene expression. This enzyme is regulated by small monomeric GTPases of the Ras and Rho families and by heterotrimeric G proteins. In addition to its phospholipase C catalytic activity, this enzyme has an N-terminal domain with guanine nucleotide exchange (GEF) activity. Mutations in this gene cause early-onset nephrotic syndrome; characterized by proteinuria, edema, and diffuse mesangial sclerosis or focal and segmental glomerulosclerosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2009]
  Transcript Variant: This variant (2) lacks two exons and has an additional novel exon at its 5' end, compared to variant 1. These differences produce a unique 5' UTR and cause translation initiation at a novel start codon, compared to variant 1. The encoded protein (isoform 2; also known as PLCepsilon1b) has a shorter and distinct N-terminus, compared to isoform 1.