SLC14A1 (NM_001146036) Human Untagged Clone

CAT#: SC327504

SLC14A1 (untagged)-Human solute carrier family 14 (urea transporter) member 1 (Kidd blood group) (SLC14A1) transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SLC14A1
• Synonyms: HsT1341; HUT11; JK; RACH1; RACH2; UT-B1; UT1; UTE
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001146036, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGGACAGCCCCACTATGGTTAGAGTGGACAGCCCCACTATGGTTAGGGGTGAAAACCAGGTTTCGC
  CATGTCAAGGGAGAAGGTGCTTCCCCAAAGCTCTTGGCTATGTCACCGGTGACATGAAAGAACTTGCCAA
  CCAGCTTAAAGACAAACCCGTGGTGCTCCAGTTCATTGACTGGATTCTCCGGGGCATATCCCAAGTGGTG
  TTCGTCAACAACCCCGTCAGTGGAATCCTGATTCTGGTAGGACTTCTTGTTCAGAACCCCTGGTGGGCTC
  TCACTGGCTGGCTGGGAACAGTGGTCTCCACTCTGATGGCCCTCTTGCTCAGCCAGGACAGGTCATTAAT
  AGCATCTGGGCTCTATGGCTACAATGCCACCCTGGTGGGAGTACTCATGGCTGTCTTTTCGGACAAGGGA
  GACTATTTCTGGTGGCTGTTACTCCCTGTATGTGCTATGTCCATGACTTGCCCAATTTTCTCAAGTGCAT
  TGAATTCCATGCTCAGCAAATGGGACCTCCCCGTCTTCACCCTCCCTTTCAACATGGCGTTGTCAATGTA
  CCTTTCAGCCACAGGACATTACAATCCATTCTTTCCAGCCAAACTGGTCATACCTATAACTACAGCTCCA
  AATATCTCCTGGTCTGACCTCAGTGCCCTGGAGTTGTTGAAATCTATACCAGTGGGAGTTGGTCAGATCT
  ATGGCTGTGATAATCCATGGACAGGGGGCATTTTCCTGGGAGCCATCCTACTCTCCTCCCCACTCATGTG
  CCTGCATGCTGCCATAGGATCATTGCTGGGCATAGCAGCGGGACTCAGTCTTTCAGCCCCATTTGAGGAC
  ATCTACTTTGGACTCTGGGGTTTCAACAGCTCTCTGGCCTGCATTGCAATGGGAGGAATGTTCATGGCGC
  TCACCTGGCAAACCCACCTCCTGGCTCTTGGCTGTGCCCTGTTCACGGCCTATCTTGGAGTCGGCATGGC
  AAACTTTATGGCTGAGGTTGGATTGCCAGCTTGTACCTGGCCCTTCTGTTTGGCCACGCTATTGTTCCTC
  ATCATGACCACAAAAAATTCCAACATCTACAAGATGCCCCTCAGTAAAGTTACTTATCCTGAAGAAAACC
  GCATCTTCTACCTGCAAGCCAAGAAAAGAATGGTGGAAAGCCCTTTGTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001146036
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001146036.2, NP_001139508.2
• RefSeq Size: 4058 bp
• RefSeq ORF: 1170 bp
• Locus ID: 6563
• Cytogenetics: 18q12.3
• Protein Families: Transmembrane
• Gene Summary: 'The protein encoded by this gene is a membrane transporter that mediates urea transport in erythrocytes. This gene forms the basis for the Kidd blood group system. [provided by RefSeq, Mar 2009]'
  Transcript Variant: This variant (3) differs in the 5' UTR and lacks an in-frame portion of the 5' coding sequence compared to variant 4. The resulting isoform (2) has a shorter N-terminus compared to isoform 1. Variants 2 and 3 both encode the same isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.