HIC5 (TGFB1I1) (NM_001164719) Human Untagged Clone

CAT#: SC327534

TGFB1I1 (untagged)-Human transforming growth factor beta 1 induced transcript 1 (TGFB1I1) transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TGFB1I1
• Synonyms: ARA55; HIC-5; HIC5; TSC-5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001164719, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCAAGGTCAGGGGCTCCCAAAGAGCGCCCTGCGGAGCCTCTCACCCCTCCCCCATCCTATGGCCACC
  AGCCACAGACAGGGTCTGGGGAGTCTTCAGGAGCCTCGGGGGACAAGGACCACCTGTACAGCACGGTATG
  CAAGCCTCGGTCCCCAAAGCCTGCAGCCCCGGCGGCCCCTCCATTCTCCTCTTCCAGCGGTGTCTTGGGT
  ACCGGGCTCTGTGAGCTAGATCGGTTGCTTCAGGAACTTAATGCCACTCAGTTCAACATCACAGATGAAA
  TCATGTCTCAGTTCCCATCTAGCAAGGTGGCTTCAGGAGAGCAGAAGGAGGACCAGTCTGAAGATAAGAA
  AAGACCCAGCCTCCCTTCCAGCCCGTCTCCTGGCCTCCCAAAGGCTTCTGCCACCTCAGCCACTCTGGAG
  CTGGATAGACTGATGGCCTCACTCTCTGACTTCCGCGTTCAAAACCATCTTCCAGCCTCTGGGCCAACTC
  AGCCACCGGTGGTGAGCTCCACAAATGAGGGCTCCCCATCCCCACCAGAGCCGACTGGCAAGGGCAGCCT
  AGACACCATGCTGGGGCTGCTGCAGTCCGACCTCAGCCGCCGGGGTGTTCCCACCCAGGCCAAAGGCCTC
  TGTGGCTCCTGCAATAAACCTATTGCTGGGCAAGTGGTGACGGCTCTGGGCCGCGCCTGGCACCCCGAGC
  ACTTCGTTTGCGGAGGCTGTTCCACCGCCCTGGGAGGCAGCAGCTTCTTCGAGAAGGATGGAGCCCCCTT
  CTGCCCCGAGTGCTACTTTGAGCGCTTCTCGCCAAGATGTGGCTTCTGCAACCAGCCCATCCGACACAAG
  ATGGTGACCGCCTTGGGCACTCACTGGCACCCAGAGCATTTCTGCTGCGTCAGTTGCGGGGAGCCCTTCG
  GAGATGAGGGTTTCCACGAGCGCGAGGGCCGCCCCTACTGCCGCCGGGACTTCCTGCAGCTGTTCGCCCC
  GCGCTGCCAGGGCTGCCAGGGCCCCATCCTGGATAACTACATCTCGGCGCTCAGCGCGCTCTGGCACCCG
  GACTGTTTCGTCTGCAGGGAATGCTTCGCGCCCTTCTCGGGAGGCAGCTTTTTCGAGCACGAGGGCCGCC
  CGTTGTGCGAGAACCACTTCCACGCACGACGCGGCTCGCTGTGCGCCACGTGTGGCCTCCCTGTGACCGG
  CCGCTGCGTGTCGGCCCTGGGTCGCCGCTTCCACCCGGACCACTTCACATGCACCTTCTGCCTGCGCCCG
  CTCACCAAGGGGTCCTTCCAGGAGCGCGCCGGCAAGCCCTACTGCCAGCCCTGCTTCCTGAAGCTCTTCG
  GCTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001164719
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164719.1, NP_001158191.1
• RefSeq Size: 1782 bp
• RefSeq ORF: 1335 bp
• Locus ID: 7041
• Cytogenetics: 16p11.2
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'This gene encodes a coactivator of the androgen receptor, a transcription factor which is activated by androgen and has a key role in male sexual differentiation. The encoded protein is thought to regulate androgen receptor activity and may have a role to play in the treatment of prostate cancer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]'
  Transcript Variant: This variant (3) differs in the 5' UTR and coding region, and initiates translation at an alternate start codon compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1. Variants 2 and 3 encode the same protein.