RASGRP 4 (RASGRP4) (NM_001146202) Human Untagged Clone

CAT#: SC327622

RASGRP4 (untagged)-Human RAS guanyl releasing protein 4 (RASGRP4) transcript variant b

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RASGRP4
• Synonyms: guanyl nucleotide releasing protein 4; RAS guanyl releasing protein 4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001146202, the custom clone sequence may differ by one or more nucleotides
  ATGAACAGAAAAGACAGTAAGAGGAAGTCCCACCAGGAATGCACCGGAAAAATAGGAGGG
  CGAGGCCGGCCCCGCCAAGTGCGCCGCCACAAGACATGCCCCAGCCCTCGGGAAATCAGC
  AAGGTCATGGCTTCCATGAACCTGGGCCTGCTGAGTGAGGGCGGCTGCAGCGAAGATGAG
  CTGCTGGAGAAATGCATCCAGTCCTTCGATTCAGCTGGCAGCCTGTGCCACGAGGACCAC
  ATGCTCAACATGGTGCTGGCCATGCACAGCTGGGTGCTGCCGTCCGCCGACCTGGCTGCC
  CGCCTGCTGACCTCATACCAGAAGGCCACAGGGGACACCCAGGAGCTGAGACGGCTGCAG
  ATCTGTCACCTGGTCAGGTACTGGCTGATGCGACACCCTGAGGTGATGCACCAGGATCCC
  CAGCTAGAAGAAGTCATAGGTCGTTTCTGGGCCACCGTGGCCCGGGAGGGCAACTCAGCC
  CAGAGAAGACTGGGAGACTCTTCTGACCTCCCAGGCCTGGGCAAAAAGCGCAAAGTGTCC
  TTGCTTTTCGACCACTTGGAGACGGGGGAGCTGGCTCAGCACCTCACCTACCTGGAGTTC
  CGGTCCTTCCAGGCTATCACGCCCCAGGACCTGCGGAGCTACGTTTTGCAGGGCTCAGTA
  CGAGGCTGCCCGGCCCTGGAGGGCTCCGTAGGTCTCAGCAACAGCGTGTCCCGCTGGGTG
  CAGGTGATGGTGCTGAGCCGTCCCGGGCCCCTACAGCGTGCACAGGTGCTGGACAAGTTC
  ATTCACGTGGCACAGAGGCTCCACCAGCTGCAGAATTTCAACACGCTGATGGCAGTCACA
  GGGGGCCTGTGTCACAGTGCCATCTCCAGACTCAAGGACTCCCATGCCCACCTGAGCCCT
  GACAGCACCAAGGCCCTCCTGGAGCTCACTGAGCTCCTTGCCTCCCACAACAACTACGCC
  CGCTACCGCCGCACCTGGGCTGGCTGCGCGGGTTTCCGGCTGCCTGTACTGGGCGTGCAC
  CTCAAGGACCTGGTGTCCCTGCATGAGGCACAGCCCGACAGGTTGCCTGACGGCCGCCTG
  CACCTACCCAAGCTGAACAACCTCTACCTGCGGCTGCAGGAGCTGGTGGCCCTCCAAGGG
  CAGCATCCACCCTGCAGCGCCAATGAGGATCTGCTGCACCTGCTCACGCTCTCCCTGGAC
  CTCTTCTACACGGAAGACGAGATCTATGAGCTTTCTTATGCCCGGGAGCCGCGTTGTCCC
  AAGAGCCTGCCACCCTCCCCCTTCAATGCACCTCTGGTGGTGGAGTGGGCCCCTGGTGTG
  ACACCCAAGCCGGACAGGGTCACACTGGGTCGGCATGTGGAGCAGCTGGTGGAGTCTGTG
  TTCAAGAATTATGACCCTGAAGGCCGAGGAACAATCTCTCAGGAGGACTTTGAGCGACTC
  TCGGGCAATTTTCCCTTCGCCTGCCATGGGCTTCACCCACCCCCACGCCAGGGGAGAGGA
  TCCTTCAGCAGAGAGGAGCTGACAGGGTACCTGCTCCGGGCCAGCGCCATCTGCTCCAAG
  TTGGGCCTGGCCTTCCTGCACACCTTCCATGAGGTCACCTTCCGAAAGCCTACCTTCTGC
  GACAGCTGCAGTGGCTTCCTCTGGGGTGTCACCAAGCAAGGCTACCGCTGTCGGGAGTGC
  GGGCTGTGTTGCCACAAACACTGCAGAGACCAGGTGAAGGTAGAATGTAAGAAGAGGCCA
  GGGGCCAAGGGCGATGCAGGACCCCCCGGAGCTCCTGTCCCATCCACACCAGCTCCCCAT
  GCCAGCTGTGGCTCCGAGGAAAATCACTCCTACACGCTATCCCTGGAGCCTGAGACTGGG
  TGCCAGCTTCGCCATGCCTGGACCCAGACTGAATCCCCACACCCTTCCTGGGAAACAGAT
  ACGGTCCCCTGCCCGGTGATGGACCCACCATCAACTGCATCCTCCAAGCTGGATTCC
  
• Restriction Sites: Please inquire
• ACCN: NM_001146202
• ORF Size: 1980 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_001146202.1, NP_001139674.1
• RefSeq Size: 3193
• RefSeq ORF: 1980
• Locus ID: 115727
• Protein Families: Druggable Genome
• Protein Pathways: MAPK signaling pathway
• Gene Summary: The protein encoded by this gene is a member of the Ras guanyl nucleotide-releasing protein (RasGRP) family of Ras guanine nucleotide exchange factors. It contains a Ras exchange motif, a diacylglycerol-binding domain, and two calcium-binding EF hands. This protein was shown to activate H-Ras in a cation-dependent manner in vitro. Expression of this protein in myeloid cell lines was found to be correlated with elevated level of activated RAS protein, and the RAS activation can be greatly enhanced by phorbol ester treatment, which suggested a role of this protein in diacylglycerol regulated cell signaling pathways. Studies of a mast cell leukemia cell line expressing substantial amounts of abnormal transcripts of this gene indicated that this gene may play an important role in the final stages of mast cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2009]
  Transcript Variant: This variant (b) uses an alternate in-frame splice junction compared to variant a. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a.