GLI1 (NM_001160045) Human Untagged Clone

CAT#: SC327684

GLI1 (untagged)-Human GLI family zinc finger 1 (GLI1) transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GLI1
• Synonyms: GLI; PAPA8; PPD1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC327684 sequence for NM_001160045 edited (data generated by NextGen Sequencing)
  
  
  ATGAGCCCATCTCTGGGATTCCCAGCCCAGATGAATCACCAAAAAGGGCCCTCGCCTTCCTTTGGGGTCC
  AGCCTTGTGGTCCCCATGACTCTGCCCGGGGTGGGATGATCCCACATCCTCAGTCCCGGGGACCCTTCCC
  AACTTGCCAGCTGAAGTCTGAGCTGGACATGCTGGTTGGCAAGTGCCGGGAGGAACCCTTGGAAGGTGAT
  ATGTCCAGCCCCAACTCCACAGGCATACAGGATCCCCTGTTGGGGATGCTGGATGGGCGGGAGGACCTCG
  AGAGAGAGGAGAAGCGTGAGCCTGAATCTGTGTATGAAACTGACTGCCGTTGGGATGGCTGCAGCCAGGA
  ATTTGACTCCCAAGAGCAGCTGGTGCACCACATCAACAGCGAGCACATCCACGGGGAGCGGAAGGAGTTC
  GTGTGCCACTGGGGGGGCTGCTCCAGGGAGCTGAGGCCCTTCAAAGCCCAGTACATGCTGGTGGTTCACA
  TGCGCAGACACACTGGCGAGAAGCCACACAAGTGCACGTTTGAAGGGTGCCGGAAGTCATACTCACGCCT
  CGAAAACCTGAAGACGCACCTGCGGTCACACACGGGTGAGAAGCCATACATGTGTGAGCACGAGGGCTGC
  AGTAAAGCCTTCAGCAATGCCAGTGACCGAGCCAAGCACCAGAATCGGACCCATTCCAATGAGAAGCCGT
  ATGTATGTAAGCTCCCTGGCTGCACCAAACGCTATACAGATCCTAGCTCGCTGCGAAAACATGTCAAGAC
  AGTGCATGGTCCTGACGCCCATGTGACCAAACGGCACCGTGGGGATGGCCCCCTGCCTCGGGCACCATCC
  ATTTCTACAGTGGAGCCCAAGAGGGAGCGGGAAGGAGGTCCCATCAGGGAGGAAAGCAGACTGACTGTGC
  CAGAGGGTGCCATGAAGCCACAGCCAAGCCCTGGGGCCCAGTCATCCTGCAGCAGTGACCACTCCCCGGC
  AGGGAGTGCAGCCAATACAGACAGTGGTGTGGAAATGACTGGCAATGCAGGGGGCAGCACTGAAGACCTC
  TCCAGCTTGGACGAGGGACCTTGCATTGCTGGCACTGGTCTGTCCACTCTTCGCCGCCTTGAGAACCTCA
  GGCTGGACCAGCTACATCAACTCCGGCCAATAGGGACCCGGGGTCTCAAACTGCCCAGCTTGTCCCACAC
  CGGTACCACTGTGTCCCGCCGCGTGGGCCCCCCAGTCTCTCTTGAACGCCGCAGCAGCAGCTCCAGCAGC
  ATCAGCTCTGCCTATACTGTCAGCCGCCGCTCCTCCCTGGCCTCTCCTTTCCCCCCTGGCTCCCCACCAG
  AGAATGGAGCATCCTCCCTGCCTGGCCTTATGCCTGCCCAGCACTACCTGCTTCGGGCAAGATATGCTTC
  AGCCAGAGGGGGTGGTACTTCGCCCACTGCAGCATCCAGCCTGGATCGGATAGGTGGTCTTCCCATGCCT
  CCTTGGAGAAGCCGAGCCGAGTATCCAGGATACAACCCCAATGCAGGGGTCACCCGGAGGGCCAGTGACC
  CAGCCCAGGCTGCTGACCGTCCTGCTCCAGCTAGAGTCCAGAGGTTCAAGAGCCTGGGCTGTGTCCATAC
  CCCACCCACTGTGGCAGGGGGAGGACAGAACTTTGATCCTTACCTCCCAACCTCTGTCTACTCACCACAG
  CCCCCCAGCATCACTGAGAATGCTGCCATGGATGCTAGAGGGCTACAGGAAGAGCCAGAAGTTGGGACCT
  CCATGGTGGGCAGTGGTCTGAACCCCTATATGGACTTCCCACCTACTGATACTCTGGGATATGGGGGACC
  TGAAGGGGCAGCAGCTGAGCCTTATGGAGCGAGGGGTCCAGGCTCTCTGCCTCTTGGGCCTGGTCCACCC
  ACCAACTATGGCCCCAACCCCTGTCCCCAGCAGGCCTCATATCCTGACCCCACCCAAGAAACATGGGGTG
  AGTTCCCTTCCCACTCTGGGCTGTACCCAGGCCCCAAGGCTCTAGGTGGAACCTACAGCCAGTGTCCTCG
  ACTTGAACATTATGGACAAGTGCAAGTCAAGCCAGAACAGGGGTGCCCAGTGGGGTCTGACTCCACAGGA
  CTGGCACCCTGCCTCAATGCCCACCCCAGTGAGGGGCCCCCACATCCACAGCCTCTCTTTTCCCATTACC
  CCCAGCCCTCTCCTCCCCAATATCTCCAGTCAGGCCCCTATACCCAGCCACCCCCTGATTATCTTCCTTC
  AGAACCCAGGCCTTGCCTGGACTTTGATTCCCCCACCCATTCCACAGGGCAGCTCAAGGCTCAGCTTGTG
  TGTAATTATGTTCAATCTCAACAGGAGCTACTGTGGGAGGGTGGGGGCAGGGAAGATGCCCCCGCCCAGG
  AACCTTCCTACCAGAGTCCCAAGTTTCTGGGGGGTTCCCAGGTTAGCCCAAGCCGTGCTAAAGCTCCAGT
  GAACACATATGGACCTGGCTTTGGACCCAACTTGCCCAATCACAAGTCAGGTTCCTATCCCACCCCTTCA
  CCATGCCATGAAAATTTTGTAGTGGGGGCAAATAGGGCTTCACATAGGGCAGCAGCACCACCTCGACTTC
  TGCCCCCATTGCCCACTTGCTATGGGCCTCTCAAAGTGGGAGGCACAAACCCCAGCTGTGGTCATCCTGA
  GGTGGGCAGGCTAGGAGGGGGTCCTGCCTTGTACCCTCCTCCCGAAGGACAGGTATGTAACCCCCTGGAC
  TCTCTTGATCTTGACAACACTCAGCTGGACTTTGTGGCTATTCTGGATGAGCCCCAGGGGCTGAGTCCTC
  CTCCTTCCCATGATCAGCGGGGCAGCTCTGGACATACCCCACCTCCCTCTGGGCCCCCCAACATGGCTGT
  GGGCAACATGAGTGTCTTACTGAGATCCCTACCTGGGGAAACAGAATTCCTCAACTCTAGTGCCTAA
  
  Clone variation with respect to NM_001160045.1
  
• Restriction Sites: Please inquire
• ACCN: NM_001160045
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001160045.1, NP_001153517.1
• RefSeq Size: 3414 bp
• RefSeq ORF: 2937 bp
• Locus ID: 2735
• Cytogenetics: 12q13.3
• Protein Families: Adult stem cells, Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Stem cell relevant signaling - DSL/Notch pathway, Transcription Factors
• Protein Pathways: Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer
• Gene Summary: 'This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]'
  Transcript Variant: This variant (1) omits the first two coding exons, compared to variant 1, resulting in transcription initiation from an internal AUG site and a N-terminally truncated protein isoform (2), also know as GLI1DeltaN. This variant reflects the transcript and protein described by Shimokawa (PubMed ID 18378682).