MICAL1 (NM_001159291) Human Untagged Clone

CAT#: SC327685

MICAL1 (untagged)-Human microtubule associated monoxygenase calponin and LIM domain containing 1 (MICAL1) transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MICAL1
• Synonyms: MICAL; MICAL-1; NICAL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001159291, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCTTCACCTACCTCCACCAACCCAGCGCATGCCCACTTTGAGAGCTTCCTGCAGGCCCAGCTGTGCC
  AGGACGTGCTGAGCAGCTTCCAGGAGCTGTGTGGGGCCCTGGGGCTGGAACCCGGTGGGGGGCTGCCCCA
  GTACCACAAGATCAAGGACCAGCTCAACTACTGGAGCGCCAAGTCACTGTGGACCAAGCTGGACAAGCGA
  GCAGGCCAGCCTGTCTACCAGCAGGGCCGGGCCTGCACCAGCACCAAGTGCCTGGTGGTGGGTGCTGGAC
  CTTGCGGGCTGCGGGTCGCTGTGGAGCTGGCGCTGCTGGGGGCCCGAGTGGTGCTGGTGGAAAAGCGCAC
  CAAGTTCTCTCGCCACAACGTGCTCCACCTCTGGCCCTTCACCATCCACGACCTGCGGGCACTCGGTGCT
  AAGAAGTTCTACGGGCGCTTCTGCACCGGCACCCTGGACCACATCAGCATCAGGCAGCTCCAGCTGCTTC
  TGCTGAAGGTAGCATTGCTGCTGGGGGTGGAAATTCACTGGGGTGTCACTTTCACTGGCCTCCAGCCCCC
  TCCTAGGAAGGGGAGTGGCTGGCGTGCCCAGCTCCAACCCAACCCCCCTGCCCAGCTGGCCAACTATGAA
  TTTGACGTCCTTATCTCGGCTGCAGGAGGTAAATTCGTCCCTGAAGGCTTCAAAGTTCGAGAAATGCGAG
  GCAAACTGGCCATTGGCATCACAGCCAACTTTGTGAATGGACGCACCGTGGAGGAGACACAGGTGCCGGA
  GATCAGTGGTGTAGCCAGGATCTACAACCAGAGCTTCTTCCAGAGCCTTCTCAAAGCCACAGGCATTGAT
  CTGGAGAACATTGTGTACTACAAGGACGACACCCACTACTTTGTGATGACAGCCAAGAAGCAGTGCCTGC
  TGCGGCTGGGGGTGCTGCGCCAGCCCTTCTGGCCCCTGGGCACTGGAGTGGCACGGGGCTTCCTGGCAGC
  CTTTGATGCAGCCTGGATGGTGAAGCGGTGGGCAGAGGGCGCTGAGTCCCTAGAGGTGTTGGCTGAGCGT
  GAGAGCCTGTACCAGCTTCTGTCACAGACATCCCCAGAAAACATGCATCGCAATGTGGCCCAGTATGGGC
  TGGACCCAGCCACCCGCTACCCCAACCTGAACCTCCGGGCAGTGACCCCCAATCAGGTACGAGACCTGTA
  TGATGTGCTAGCCAAGGAGCCTGTGCAGAGGAACAACGACAAGACAGATACAGGGATGCCAGCCACCGGG
  TCGGCAGGCACCCAGGAGGAGCTGCTACGCTGGTGCCAGGAGCAGACAGCTGGGTACCCGGGAGTCCACG
  TCTCCGATTTGTCTTCCTCCTGGGCTGATGGGCTAGCTCTGTGTGCCCTGGTGTACCGGCTGCAGCCTGG
  CCTGCTGGAACCCTCAGAGCTGCAGGGGCTGGGAGCTCTGGAAGCAACTGCTTGGGCACTAAAGGTGGCA
  GAGAATGAGCTGGGCATCACACCGGTGGTGTCTGCACAGGCCGTGGTAGCAGGGAGTGACCCACTGGGCC
  TCATTGCCTACCTCAGCCACTTCCACAGTGCCTTCAAGAGCATGGCCCACAGCCCAGGCCCTGTCAGCCA
  GGCCTCCCCAGGGACCTCCAGTGCTGTATTATTCCTTAGTAAACTTCAGAGGACCCTGCAGCGATCCCGG
  GCCAAGGAAAATGCAGAGGATGCTGGTGGCAAGAAGCTGCGCTTGGAGATGGAGGCCGAGACCCCAAGTA
  CTGAGGTGCCACCTGACCCAGAGCCTGGTGTACCCCTGACACCCCCATCCCAACACCAGGAGGCCGGTGC
  TGGGGACCTGTGTGCACTTTGTGGGGAACACCTCTATGTCCTGGAACGCCTCTGTGTCAACGGCCATTTC
  TTCCACCGGAGCTGCTTCCGCTGCCATACCTGTGAGGCCACACTGTGGCCAGGTGGCTACGAGCAGCACC
  CAGGAGATGGACATTTCTACTGCCTCCAGCACCTGCCCCAGACAGACCACAAAGCGGAAGGCAGCGATAG
  AGGCCCTGAGAGTCCGGAGCTCCCCACACCAAGTGAGAATAGCATGCCACCAGGCCTCTCAACTCCCACA
  GCCTCGCAGGAGGGGGCCGGTCCTGTTCCAGATCCCAGCCAGCCCACCCGTCGGCAGATCCGCCTCTCCA
  GCCCGGAGCGCCAGCGGTTGTCCTCCCTTAACCTTACCCCTGACCCGGAAATGGAGCCTCCACCCAAGCC
  TCCCCGCAGCTGCTCCGCCTTGGCCCGCCACGCCCTGGAGAGCAGCTTTGTGGGCTGGGGCCTGCCAGTC
  CAGAGCCCTCAAGCTCTTGTGGCCATGGAGAAGGAGGAAAAAGAGAGTCCCTTCTCCAGTGAAGAGGAAG
  AAGAAGATGTGCCTTTGGACTCAGATGTGGAACAGGCCCTGCAGACCTTTGCCAAGACCTCAGGCACCAT
  GAATAACTACCCAACATGGCGTCGGACTCTGCTGCGCCGTGCGAAGGAGGAGGAGATGAAGAGGTTCTGC
  AAGGCCCAGACCATCCAACGGCGACTAAATGAGATTGAGGCTGCCTTGAGGGAGCTAGAGGCCGAGGGCG
  TGAAGCTGGAGCTGGCCTTGAGGCGCCAGAGCAGTTCCCCAGAACAGCAAAAGAAACTATGGGTAGGACA
  GCTGCTACAGCTCGTTGACAAGAAAAACAGCCTGGTGGCTGAGGAGGCCGAGCTCATGATCACGGTGCAG
  GAATTGAATCTGGAGGAGAAACAGTGGCAGCTGGACCAGGAGCTACGAGGCTACATGAACCGGGAAGAAA
  ACCTAAAGACAGCTGCTGATCGGCAGGCTGAGGACCAGGTCCTGAGGAAGCTGGTGGATTTGGTCAACCA
  GAGAGATGCCCTCATCCGCTTCCAGGAGGAGCGCAGGCTCAGCGAGCTGGCCTTGGGGACAGGGGCCCAG
  GGCTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001159291
• ORF Size: 2946 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_001159291.1, NP_001152763.1
• RefSeq Size: 3403
• RefSeq ORF: 2946
• Locus ID: 64780
• Gene Summary: This gene encodes an enzyme that oxidizes methionine residues on actin, thereby promoting depolymerization of actin filaments. This protein interacts with and regulates signalling by NEDD9/CAS-L (neural precursor cell expressed, developmentally down-regulated 9). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]
  Transcript Variant: This variant (2) contains an alternate 5' exon and thus differs in its 5' UTR and initiates translation at a downstream in-frame start codon, and it lacks an alternate in-frame exon in the central coding region, compared to variant 3. The encoded isoform (2, also known as MICAL-1b) is shorter at the N-terminus, compared to isoform 3.