ARHGAP4 (NM_001164741) Human Untagged Clone

CAT#: SC327689

ARHGAP4 (untagged)-Human Rho GTPase activating protein 4 (ARHGAP4) transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ARHGAP4
• Synonyms: C1; p115; RGC1; RhoGAP4; SrGAP4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001164741, the custom clone sequence may differ by one or more nucleotides
  ATGGCCGCTCACGGGAAGCTGCGGCGGGAGCGGGGGCTGCAGGCTGAGTATGAGACGCAA
  GTCAAAGAGATGCGCTGGCAGCTGAGCGAGCAGCTGCGCTGCCTGGAGCTGCAGGGCGAG
  CTGCGGCGGGAGTTGCTGCAGGAGCTGGCAGAGTTCATGCGGCGCCGCGCTGAGGTGGAG
  CTGGAATACTCCCGGGGCCTGGAAAAGCTGGCCGAGCGCTTCTCCAGCCGTGGAGGCCGC
  CTGGGGAGCAGCCGGGAGCACCAAAGCTTCCGGAAGGAGCCGTCCCTCCTGTCGCCCTTG
  CACTGCTGGGCGGTGCTGCTGCAGCACACGCGGCAGCAGAGCCGGGAGAGCGCGGCCCTG
  AGTGAGGTGCTGGCCGGGCCCCTGGCCCAGCGCCTGAGTCACATTGCAGAGGACGTGGGG
  CGCCTGGTCAAGAAGAGCAGGGATCTGGAGCAGCAGCTGCAGGATGAGCTCCTGGAGGTG
  GTCTCAGAGCTCCAGACGGCCAAGAAGACGTACCAGGCATATCACATGGAGAGCGTGAAT
  GCCGAGGCCAAGCTCCGGGAGGCCGAGCGGCAGGAGGAGAAGCGGGCAGGCCGGAGTGTC
  CCCACCACCACCGCTGGTGCCACTGAGGCAGGGCCCCTCCGCAAGAGCTCCCTCAAGAAG
  GGAGGGAGGCTGGTGGAGAAGCTCTGGCCCCCGCAGAGGCCTGTGGCCGCTTCCAGCTGT
  GCACCTGTGTGCTGGCTCCAAGCTGGGTTTCTCGTGCACCCTCCATGGTGGGGTGCCATG
  TGCGCACCTTCCACTCATCAGCGGCAGGCCAAGTTCATGGAGCACAAACTCAAGTGCACA
  AAGGCGCGCAACGAGTACCTGCTTAGCCTGGCTAGTGTCAACGCTGCTGTCAGTAACTAC
  TACCTGCATGACGTCTTGGACCTCATGGACTGCTGTGACACAGGGTTCCACCTGGCCCTG
  GGGCAGGTGCTCCGGAGCTACACGGCCGCTGAGAGCCGCACCCAAGCCTCCCAAGTGCAG
  GGCCTGGGCAGCCTGGAAGAAGCTGTGGAGGCCCTGGATCCTCCAGGGGACAAAGCCAAG
  GTTCTCGAGGTGCATGCTACCGTCTTCTGTCCCCCGCTGCGCTTTGACTACCACCCCCAT
  GATGGGGATGAGGTGGCTGAGATCTGCGTTGAAATGGAGCTGCGGGACGAGATTCTGCCC
  AGAGCCCAGAACATCCAGAGCCGCCTGGACCGACAGACCATTGAGACAGAGGAGGTGAAC
  AAGACTCTGAAGGCGACACTGCAGGCCCTGCTGGAGGTGGTGGCCTCGGATGACGGGGAT
  GTGCTTGATTCCTTCCAGACCAGCCCCTCCACCGAGTCCCTCAAGTCCACCAGCTCAGAC
  CCAGGCAGCCGGCAGGCGGGCCGGAGGCGCGGCCAGCAGCAGGAGACCGAAACCTTCTAC
  CTCACGAAGCTCCAGGAGTATCTGAGTGGACGGAGCATCCTCGCCAAGCTGCAGGCCAAG
  CACGAGAAGCTGCAGGAGGCCCTTCAGCGAGGTGACAAGGAGGAGCAGGAGGTGTCTTGG
  ACCCAGTACACACAGAGAAAATTCCAGAAGAGCCGCCAGCCCCGCCCCAGCTCCCAGTAT
  AACCAGAGACTCTTTGGGGGAGACATGGAGAAGTTTATCCAGAGCTCAGGCCAGCCTGTG
  CCCCTGGTGGTGGAGAGCTGCATTCGCTTCATCAACCTCAATGGCCTGCAGCATGAAGGC
  ATCTTCCGGGTATCGGGTGCCCAGCTCCGGGTCTCAGAGATCCGTGATGCCTTCGAGAGA
  GGGGAGGACCCACTGGTGGAGGGCTGCACTGCCCATGACCTGGACTCGGTGGCCGGGGTG
  CTGAAGCTCTACTTCCGGAGCCTGGAGCCCCCACTCTTCCCCCCAGACCTGTTCGGCGAG
  CTGCTGGCTTCTTCGGAGCTGGAGGCCACAGCGGAGAGGGTGGAGCACGTGAGCCGCCTG
  CTGTGGCGGCTGCCCGCGCCGGTGCTGGTGGTTCTGCGCTACCTCTTCACCTTCCTCAAC
  CACCTGGCCCAGTACAGCGATGAGAACATGATGGACCCCTACAACCTGGCCGTGTGCTTC
  GGGCCCACGCTGCTACCGGTGCCCGCTGGGCAGGACCCGGTGGCGCTGCAGGGCCGGGTG
  AACCAGCTGGTGCAGACGCTCATAGTGCAGCCCGATCGGGTCTTCCCGCCCCTGACCTCG
  CTGCCTGGCCCCGTCTACGAGAAGTGCATGGCACCGCCTTCCGCCAGCTGCCTGGGGGAC
  GCCCAGCTGGAGAGCCTGGGGGCGGACAATGAGCCGGAGCTGGAAGCCGAGATGCCCGCA
  CAGGAGGATGACCTGGAGGGGGTCGTGGAGGCTGTGGCCTGCTTTGCCTACACGGGCCGC
  ACAGCCCAGGAGCTGAGCTTCCGGCGGGGGGACGTACTGCGGCTGCACGAGAGGGCCTCG
  AGCGACTGGTGGCGGGGGGAGCACAACGGCATGCGGGGCCTCATCCCCCACAAGTATATC
  ACGCTGCCCGCCGGGACGGAGAAGCAGGTGGTGGGCGCAGGGCTGCAGACTGCAGGGGAG
  TCTGGGAGCAGTCCCGAGGGCCTCCTGGCATCGGAGCTGGTCCACCGGCCAGAGCCATGC
  ACCTCACCTGAGGCCATGGGACCCTCTGGACACAGACGACGCTGCTTGGTCCCAGCCTCC
  CCAGAGCAACACGTGGAGGTGGATAAGGCTGTGGCACAGAACATGGACTCTGTGTTTAAG
  GAGCTCTTGGGAAAGACCTCTGTCCGCCAGGGCCTTGGGCCAGCATCTACCACCTCTCCC
  AGTCCTGGGCCCCGAAGCCCAAAGGCCCCGCCCAGCAGCCGCCTGGGCAGGAACAAAGGC
  TTCTCCCGGGGCCCTGGGGCCCCAGCCTCACCCTCAGCTTCCCACCCCCAGGGCCTAGAC
  ACGACCCCCAAGCCACAC
  
• Restriction Sites: Please inquire
• ACCN: NM_001164741
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164741.1, NP_001158213.1
• RefSeq Size: 3405 bp
• RefSeq ORF: 2961 bp
• Locus ID: 393
• Cytogenetics: Xq28
• Gene Summary: 'This gene encodes a member of the rhoGAP family of proteins which play a role in the regulation of small GTP-binding proteins belonging to the RAS superfamily. The protein encoded by the orthologous gene in rat is localized to the Golgi complex and can redistribute to microtubules. The rat protein stimulates the activity of some Rho GTPases in vitro. Genomic deletions of this gene and a neighboring gene have been found in patients with nephrogenic diabetes insipidus. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).