PEG3 (NM_001146187) Human Untagged Clone

CAT#: SC327713

PEG3 (untagged)-Human paternally expressed 3 (PEG3) transcript variant 5

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PEG3
• Synonyms: PW1; ZKSCAN22; ZNF904; ZSCAN24
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001146187, the custom clone sequence may differ by one or more nucleotides
  ATGTACCAACCAGAAGACGACAACAACAGTGACGTGACCAGCGACGACGACATGACCCGG
  AACAGAAGAGAGTCCTCACCACCTCACTCAGTCCATTCTTTCAGTGGTGACCGGGACTGG
  GACCGGAGGGGCAGAAGCAGAGACATGGAGCCACGAGACCGCTGGTCCCACACCAGGAAC
  CCAAGAAGCAGGATGCCTCCGCGGGATCTTTCCCTTCCTGTGGTGGCGAAAACAAGCTTT
  GAAATGGACAGAGAGGACGACAGGGACTCCAGGGCTTATGAGTCCCGATCTCAGGATGCT
  GAATCATACCAAAATGTGGTGGACCTCGCTGAGGACAGGAAACCTCACAACACAATCCAG
  GACAACATGGAAAACTACAGGAAGCTGCTCTCCCTCGGAGTGCAGCTTGCTGAAGACGAT
  GGCCACTCCCACATGACGCAGGGCCACTCATCAAGATCCAAGAGAAGTGCCTACCCAAGC
  ACCAGTCGAGGTCTAAAAACTATGCCTGAAGCCAAAAAATCAACCCACCGGCGGGGGATT
  TGTGAAGATGAATCTTCCCACGGAGTGATAATGGAAAAATTCATCAAGGATGTGTCACGC
  AGTTCCAAATCGGGAAGAGCAAGGGAGTCAAGCGACCGGTCACAGAGATTCCCCAGAATG
  TCAGATGATAACTGGAAGGACATTTCATTGAACAAGAGGGAGTCAGTGATCCAGCAGCGG
  GTTTATGAAGGGAATGCATTTAGGGGAGGCTTTAGGTTTAATTCAACCCTTGTTTCCAGA
  AAGAGAGTTCTTGAAAGAAAGAGGCGCTATCATTTTGACACAGATGGGAAGGGCTCGATT
  CACGATCAAAAAGGCTGTCCCAGGAAGAAGCCCTTTGAATGTGGTAGTGAGATGAGAAAA
  GCCATGAGCGTGAGCAGCCTGAGCAGCCTCAGCTCCCCCTCCTTTACCGAGTCACAGCCA
  ATTGATTTTGGGGCAATGCCATATGTATGTGATGAGTGTGGGAGGTCGTTCAGTGTCATC
  TCAGAATTTGTTGAGCACCAGATCATGCATACTAGAGAGAACCTCTATGAGTATGGTGAG
  TCCTTTATCCACAGTGTGGCTGTCAGTGAAGTTCAGAAAAGTCAGGTTGGAGGGAAACGT
  TTTGAATGTAAGGACTGTGGAGAGACCTTCAATAAGAGTGCCGCCTTGGCTGAACATCGG
  AAGATTCATGCTAGAGGTTATCTTGTGGAATGTAAGAATCAGGAATGTGAGGAAGCCTTC
  ATGCCTAGCCCCACCTTTAGTGAGCTTCAGAAAATATATGGCAAAGACAAATTCTACGAG
  TGCAGGGTGTGTAAGGAAACCTTCCTTCATAGTTCTGCCCTGATTGAGCACCAGAAAATC
  CACTTTGGGGATGACAAAGATAATGAGCGTGAACATGAACGTGAACGTGAACGTGAGCGC
  GGGGAAACCTTTAGGCCCAGCCCAGCCCTTAATGAGTTTCAGAAAATGTATGGTAAAGAG
  AAAATGTACGAATGTAAGGTGTGTGGGGAGACTTTCCTTCATAGCTCATCCCTGAAAGAA
  CATCAGAAAATCCATACTAGAGGGAACCCATTTGAAAACAAGGGTAAAGTGTGTGAGGAA
  ACCTTTATTCCTGGTCAGTCCCTTAAAAGGCGTCAGAAAACTTACAATAAGGAGAAGCTC
  TGTGACTTTACAGATGGCCGGGATGCCTTCATGCAAAGCTCAGAGCTCAGTGAGCATCAG
  AAAATTCATTCTCGAAAGAACCTCTTTGAAGGCAGAGGGTATGAGAAATCTGTCATTCAT
  AGTGGGCCATTCACTGAATCTCAGAAGAGTCATACTATAACAAGACCTCTTGAAAGTGAT
  GAGGACGAAAAGGCGTTCACCATTAGCTCTAACCCCTATGAAAACCAGAAGATTCCCACT
  AAGGAAAATGTCTATGAGGCAAAATCATATGAGAGGTCTGTTATTCATAGCTTAGCCTCT
  GTGGAAGCTCAGAAAAGTCACAGTGTAGCAGGGCCCAGTAAACCAAAAGTAATGGCAGAG
  TCTACCATTCAGAGCTTCGATGCTATCAACCATCAGAGAGTTCGTGCTGGAGGGAACACC
  TCTGAAGGAAGGGAATACAGTAGGTCTGTTATCCATAGCTTAGTGGCTTCCAAACCTCCA
  AGAAGTCACAATGGAAATGAATTGGTGGAATCTAATGAGAAGGGAGAATCCTCCATTTAT
  ATCTCAGACCTTAATGATAAGCGACAGAAGATTCCTGCCAGAGAGAACCCTTGTGAAGGG
  GGCAGTAAGAATCGCAACTATGAAGACTCTGTCATACAGAGTGTATTCCGTGCCAAACCT
  CAGAAAAGTGTTCCTGGAGAGGGATCTGGTGAGTTTAAGAAGGATGGCGAATTCTCTGTT
  CCCAGCTCAAATGTCCGTGAATACCAGAAGGCTCGTGCTAAAAAGAAATACATTGAGCAT
  AGGAGCAATGAGACCTCTGTAATTCACTCTCTGCCTTTTGGTGAACAAACATTTCGCCCT
  CGAGGGATGCTCTATGAATGTCAGGAGTGTGGGGAGTGCTTTGCTCATAGCTCTGACCTC
  ACTGAGCACCAGAAGATTCATGATAGGGAGAAGCCCTCTGGAAGCAGAAACTATGAATGG
  TCTGTCATTCGCAGCTTGGCCCCTACTGACCCTCAAACAAGTTACGCCCAAGAGCAGTAT
  GCTAAAGAGCAAGCGCGGAACAAATGTAAGGACTTCAGACAATTTTTTGCTACCAGCGAA
  GACCTCAACACAAACCAGAAAATCTATGACCAAGAGAAGTCTCATGGCGAGGAGTCTCAA
  GGCGAGAATACTGATGGGGAGGAGACCCACAGCGAGGAGACCCATGGTCAGGAGACAATT
  GAAGACCCTGTCATTCAAGGCTCAGACATGGAAGACCCTCAGAAGGATGACCCTGATGAC
  AAAATCTATGAATGTGAGGACTGTGGCCTGGGCTTTGTGGATCTCACAGACCTCACAGAC
  CATCAGAAAGTCCACAGCAGGAAGTGCCTGGTTGACAGTCGGGAGTACACACATTCTGTA
  ATTCACACCCATTCCATCAGCGAGTATCAGAGAGATTACACTGGAGAGCAGCTGTATGAA
  TGTCCAAAGTGTGGGGAATCTTTTATTCATAGCTCATTCCTTTTCGAGCATCAGAGAATC
  CATGAACAAGACCAGTTGTATTCCATGAAGGGGTGTGATGATGGTTTTATTGCCCTCTTG
  CCCATGAAGCCACGGAGGAATCGTGCTGCAGAGAGGAATCCTGCTCTTGCTGGGTCGGCC
  ATTCGATGCCTTTTGTGTGGACAAGGCTTCATTCATAGCTCTGCCCTTAATGAGCATATG
  AGACTTCATAGGGAAGATGATTTACTGGAGCAGAGCCAGATGGCTGAGGAAGCTATCATT
  CCAGGCTTAGCCCTCACTGAGTTTCAGAGAAGTCAGACCGAAGAGAGACTCTTTGAATGT
  GCAGTCTGTGGAGAATCTTTCGTCAACCCAGCAGAACTTGCAGATCACGTAACTGTTCAT
  AAGAATGAGCCCTATGAGTACGGGTCCTCCTATACTCACACCTCATTTCTTACTGAGCCC
  CTCAAAGGAGCTATACCATTCTATGAATGCAAGGATTGTGGTAAGTCCTTTATTCATAGC
  ACAGTCCTCACTAAACATAAGGAGCTTCATCTGGAAGAAGAAGAAGAAGATGAAGCAGCA
  GCAGCTGCAGCAGCAGCAGCCCAGGAAGTTGAAGCCAATGTCCATGTTCCACAAGTAGTT
  CTGAGGATTCAGGGCTTAAACGTAGAGGCTGCTGAGCCAGAAGTGGAGGCTGCCGAGCCA
  GAAGTGGAGGCTGCTGAGCCAGAAGTGGAGGCTGCTGAGCCAAACGGAGAGGCTGAAGGG
  CCAGATGGAGAGGCTGCAGAGCCCATTGGAGAGGCTGGACAGCCAAATGGAGAGGCCGAG
  CAGCCAAATGGGGATGCTGATGAGCCAGATGGTGCAGGTATTGAAGACCCAGAAGAAAGA
  GCTGAAGAGCCAGAGGGAAAAGCTGAAGAGCCAGAGGGAGATGCCGACGAGCCTGACGGT
  GTGGGAATTGAAGACCCAGAAGAAGGTGAAGATCAAGAGATTCAGGTAGAAGAACCATAC
  TATGACTGCCATGAATGCACAGAAACCTTCACTTCCAGCACAGCATTCAGTGAACACCTG
  AAAACTCATGCCAGCATGATCATATTTGAGCCTGCAAATGCCTTTGGGGAGTGCTCAGGC
  TACATCGAACGTGCCAGCACCAGCACAGGTGGTGCCAATCAAGCTGATGAGAAGTACTTC
  AAATGTGACGTCTGTGGGCAGCTCTTCAATGACCGCCTGTCCCTCGCCAGACACCAGAAT
  ACCCACACTGGC
  
• Restriction Sites: Please inquire
• ACCN: NM_001146187
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001146187.1, NP_001139659.1
• RefSeq Size: 8381 bp
• RefSeq ORF: 4395 bp
• Locus ID: 5178
• Cytogenetics: 19q13.43
• Protein Families: Transcription Factors
• Gene Summary: 'In human, ZIM2 and PEG3 are treated as two distinct genes though they share multiple 5' exons and a common promoter and both genes are paternally expressed (PMID:15203203). Alternative splicing events connect their shared 5' exons either with the remaining 4 exons unique to ZIM2, or with the remaining 2 exons unique to PEG3. In contrast, in other mammals ZIM2 does not undergo imprinting and, in mouse, cow, and likely other mammals as well, the ZIM2 and PEG3 genes do not share exons. Human PEG3 protein belongs to the Kruppel C2H2-type zinc finger protein family. PEG3 may play a role in cell proliferation and p53-mediated apoptosis. PEG3 has also shown tumor suppressor activity and tumorigenesis in glioma and ovarian cells. Alternative splicing of this PEG3 gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2009]'
  Transcript Variant: This variant (5) is missing a 5' non-coding exon, and uses alternate splice sites at several coding exons compared to variant 1. This results in the use of an in-frame downstream start codon, and an isoform (3) with a shorter N-terminus and two additional internal aa compared to isoform 1. Variants 5, 9-22, and 30 all encode the same isoform (3).