DNAJB12 (NM_001002762) Human Untagged Clone

CAT#: SC327834

DNAJB12 (untagged)-Human DnaJ (Hsp40) homolog subfamily B member 12 (DNAJB12) transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DNAJB12
• Synonyms: DJ10
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001002762, the custom clone sequence may differ by one or more nucleotides
  ATGTCATCACTCCGCGCCCGGCTGCCCGCGACGCGCCGGCGGGTGGCGCAGCCCTTCGCT
  CGCCCGGCCTCCCCCTCCCTGGTTCCGCGTTCTGGTTCCGCCATGGAATCCAACAAGGAT
  GAAGCTGAGCGCTGTATCAGCATCGCCCTCAAGGCCATCCAGAGCAACCAGCCCGACCGG
  GCGCTCCGCTTCCTGGAGAAGGCACAGCGGCTGTATCCGACGCCGCGAGTTCGCGCCCTG
  ATTGAGTCCCTCAACCAGAAACCACAGACTGCCGGTGACCAACCCCCACCCACAGACACA
  ACCCATGCCACCCACAGGAAAGCAGGTGGGACCGATGCCCCCTCGGCCAACGGTGAAGCT
  GGAGGAGAGAGCACCAAAGGCTACACTGCAGAACAGGTTGCAGCTGTGAAAAGGGTCAAG
  CAATGTAAAGATTACTATGAGATCCTGGGGGTGAGCAGAGGGGCCTCGGATGAGGACCTG
  AAGAAGGCCTACCGCAGACTGGCCCTCAAATTCCACCCAGACAAGAACCACGCACCTGGT
  GCCACTGAAGCCTTCAAAGCCATTGGCACAGCATATGCGGTACTCAGCAACCCGGAGAAG
  AGGAAGCAGTATGACCAGTTCGGCGATGACAAGAGCCAGGCGGCCCGGCACGGCCATGGG
  CATGGGGATTTCCACCGTGGCTTTGAGGCCGACATCTCCCCTGAAGACCTCTTCAACATG
  TTCTTTGGCGGCGGCTTCCCTTCTAGTAACGTCCACGTCTACAGCAACGGCCGCATGCGC
  TATACCTACCAGCAAAGGCAGGACCGCAGGGACAACCAGGGTGATGGCGGGCTAGGGGTG
  TTTGTGCAGCTGATGCCTATCCTCATCCTGATTCTCGTGTCAGCTCTCAGCCAGCTCATG
  GTCTCCAGTCCACCCTACAGTCTGAGTCCAAGACCGTCCGTGGGCCACATCCACAGGCGA
  GTCACTGACCACCTGGGTGTCGTCTACTATGTGGGAGACACTTTCTCCGAAGAGTACACA
  GGCTCCAGCCTCAAAACAGTCGAGCGGAATGTGGAAGATGATTATATCGCCAACCTCCGG
  AACAACTGCTGGAAGGAGAAGCAGCAGAAGGAAGGCTTGCTGTACCGGGCACGCTACTTT
  GGCGACACAGATATGTACCACAGAGCACAGAAGATGGGCACCCCCAGCTGCAGCCGACTG
  TCAGAGGTGCAGGCCTCCCTGCATGGA
  
• Restriction Sites: Please inquire
• ACCN: NM_001002762
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001002762.2, NP_001002762.2
• RefSeq Size: 4377 bp
• RefSeq ORF: 1230 bp
• Locus ID: 54788
• Cytogenetics: 10q22.1
• Protein Families: Transmembrane
• Gene Summary: DNAJB12 belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus; a glycine/phenylalanine (G/F)-rich region; and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain (Ohtsuka and Hata, 2000 [PubMed 11147971]). [supplied by OMIM, Mar 2008]
  Transcript Variant: This variant (1) represents the longer transcript. Variants 1, 2 and 4 encode the same protein. CCDS Note: The coding region has been updated to shorten the N-terminus to one that is more supported by conservation.