DUSP7 (NM_001947) Human Untagged Clone

CAT#: SC327842

DUSP7 (untagged)-Human dual specificity phosphatase 7 (DUSP7)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DUSP7
• Synonyms: MKPX; PYST2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001947, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAAAAACCAGCTCCGCGGCCCCCCAGCGCGGGCGCACATGTCGACTTCGGGGGCGGCGGCGGCTGGGG
  GCACCCGGGCGGGGTCCGAGCCCGGTGCGGGGTCGGGGTCCGGCGCAGGCACCGGGGCGGGCGCGGCGAC
  GGGGGCAGGGGCCATGCCCTGCAAGAGCGCCGAGTGGCTGCAGGAGGAGCTGGAGGCGCGCGGCGGCGCG
  TCCTTGCTGCTGCTCGACTGCCGGCCGCACGAGCTCTTCGAGTCGTCGCACATCGAGACGGCCATCAACC
  TGGCCATCCCGGGCCTCATGTTGCGCCGCCTGCGCAAGGGCAACCTGCCCATCCGCTCCATCATCCCCAA
  CCACGCCGACAAGGAGCGCTTCGCCACGCGCTGCAAGGCGGCCACCGTGCTGCTCTACGACGAGGCCACG
  GCCGAGTGGCAGCCCGAGCCCGGCGCTCCCGCCTCCGTGCTCGGCCTGCTCCTACAGAAGCTGCGCGACG
  ACGGCTGCCAGGCCTACTACCTCCAAGGTGGTTTCAACAAGTTTCAAACAGAGTACTCTGAGCACTGCGA
  GACCAACGTGGACAGCTCTTCCTCGCCGAGCAGCTCGCCACCCACCTCAGTGCTGGGCCTGGGGGGCCTG
  CGCATCAGCTCTGACTGCTCCGACGGCGAGTCGGACCGAGAGCTGCCCAGCAGTGCCACCGAGTCAGACG
  GCAGCCCTGTGCCATCCAGCCAACCAGCCTTCCCTGTCCAGATCCTGCCCTACCTCTACCTCGGCTGCGC
  CAAGGACTCCACCAACCTGGACGTGCTCGGCAAGTATGGCATCAAGTATATCCTCAATGTCACACCCAAC
  CTACCCAACGCCTTCGAGCACGGCGGCGAGTTCACCTACAAGCAGATCCCCATCTCTGACCACTGGAGCC
  AGAACCTCTCCCAGTTCTTCCCTGAGGCCATCAGCTTCATTGACGAAGCCCGCTCCAAGAAGTGTGGTGT
  CCTGGTGCACTGCCTGGCAGGCATCAGCCGCTCAGTGACGGTCACTGTGGCCTATCTGATGCAGAAGATG
  AACCTGTCACTCAACGACGCCTACGACTTTGTCAAGAGGAAAAAGTCCAACATCTCGCCCAACTTCAACT
  TCATGGGGCAGCTGCTGGACTTTGAGCGGACGCTGGGGCTAAGCAGCCCGTGCGACAACCACGCGTCGAG
  TGAGCAGCTCTACTTTTCCACGCCCACCAACCACAACCTGTTCCCACTCAATACGCTGGAGTCCACGTGA
  
  
• Restriction Sites: SgfI-NotI
• ACCN: NM_001947
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001947.3, NP_001938.2
• RefSeq Size: 3248 bp
• RefSeq ORF: 1260 bp
• Locus ID: 1849
• Cytogenetics: 3p21.2
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: MAPK signaling pathway
• Gene Summary: 'Dual-specificity phosphatases (DUSPs) constitute a large heterogeneous subgroup of the type I cysteine-based protein-tyrosine phosphatase superfamily. DUSPs are characterized by their ability to dephosphorylate both tyrosine and serine/threonine residues. DUSP7 belongs to a class of DUSPs, designated MKPs, that dephosphorylate MAPK (mitogen-activated protein kinase) proteins ERK (see MIM 601795), JNK (see MIM 601158), and p38 (see MIM 600289) with specificity distinct from that of individual MKP proteins. MKPs contain a highly conserved C-terminal catalytic domain and an N-terminal Cdc25 (see MIM 116947)-like (CH2) domain. MAPK activation cascades mediate various physiologic processes, including cellular proliferation, apoptosis, differentiation, and stress responses (summary by Patterson et al., 2009 [PubMed 19228121]).[supplied by OMIM, Dec 2009]'