PLCL1 (NM_006226) Human Untagged Clone

CAT#: SC328044

PLCL1 (untagged)-Human phospholipase C-like 1 (PLCL1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PLCL1
• Synonyms: PLCE; PLCL; PLDL1; PPP1R127; PRIP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_006226, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCGAGGGCGCGGCCGGCAGGGAGGATCCGGCGCCGCCCGACGCGGCGGGGGGCGAAGACGACCCCC
  GAGTGGGCCCGGATGCCGCCGGGGACTGCGTGACGGCGGCCTCTGGGGGCCGGATGAGGGACCGTCGCAG
  CGGGGTCGCACTGCCAGGCGCCGCGGGGACCCCAGCGGACAGCGAGGCGGGCCTCCTGGAGGCAGCACGG
  GCGACCCCCCGGCGCAGCAGCATCATCAAGGATCCTTCAAACCAAAAATGTGGTGGAAGAAAGAAAACCG
  TGTCTTTCAGCAGCATGCCATCGGAAAAGAAAATTAGCAGTGCAAATGACTGCATCAGCTTCATGCAAGC
  TGGCTGTGAGTTGAAGAAAGTCCGGCCAAATTCTCGCATTTACAACCGTTTTTTCACTCTGGACACAGAC
  CTTCAAGCTCTTCGCTGGGAACCTTCAAAGAAAGACCTCGAGAAAGCCAAGCTTGATATTTCTGCCATAA
  AAGAGATCAGACTGGGGAAAAACACGGAAACATTTAGAAACAATGGCCTTGCTGACCAGATCTGTGAGGA
  CTGTGCCTTTTCCATACTCCACGGGGAAAACTATGAGTCTCTGGACCTAGTTGCCAATTCAGCAGATGTG
  GCAAACATCTGGGTGTCTGGGTTACGGTACCTGGTTTCTCGAAGTAAGCAGCCTCTTGATTTTATGGAGG
  GCAACCAGAACACACCACGGTTCATGTGGTTGAAAACAGTGTTTGAAGCAGCAGATGTTGATGGGAATGG
  GATTATGTTGGAAGACACCTCTGTAGAGTTAATAAAACAACTCAACCCTACTCTGAAGGAAGCCAAGATC
  AGGTTAAAGTTTAAAGAAATCCAGAAGAGCAAGGAAAAACTAACCACCCGCGTGACCGAAGAGGAATTTT
  GTGAAGCTTTTTGTGAACTTTGCACCAGGCCAGAAGTGTATTTCTTACTTGTACAGATATCTAAAAACAA
  AGAATATTTGGATGCCAATGATCTCATGCTCTTTTTAGAAGCTGAGCAAGGAGTCACCCATATCACCGAG
  GATATATGCTTAGACATCATAAGGAGATACGAACTTTCTGAAGAGGGACGTCAAAAAGGGTTTCTTGCAA
  TTGATGGCTTTACCCAGTATTTATTGTCATCAGAATGTGACATTTTTGATCCTGAGCAAAAGAAGGTTGC
  CCAAGATATGACCCAGCCATTATCTCACTACTATATCAATGCCTCTCATAACACCTATCTAATAGAAGAC
  CAGTTCAGGGGGCCAGCTGACATCAATGGGTACATTAGAGCTTTGAAAATGGGCTGTCGAAGCGTTGAAC
  TCGATGTAAGTGATGGTTCAGATAATGAACCAATCCTTTGTAATCGAAATAACATGACAACCCATGTTTC
  CTTTCGAAGTGTCATAGAGGTAATAAATAAATTTGCCTTTGTTGCTTCTGAATACCCACTCATTCTTTGC
  TTGGGAAATCACTGCTCCTTGCCGCAGCAGAAGGTAATGGCTCAACAGATGAAAAAGGTCTTTGGCAATA
  AACTCTATACTGAAGCACCTTTGCCCTCAGAATCCTACCTCCCATCACCAGAAAAATTAAAAAGAATGAT
  CATTGTGAAAGGAAAGAAGTTGCCTTCTGATCCAGATGTGTTAGAAGGAGAAGTAACAGATGAAGATGAA
  GAAGCTGAAATGTCTCGAAGGATGTCGGTAGATTACAATGGTGAGCAGAAGCAAATCCGACTCTGTAGGG
  AGCTCTCTGATTTGGTGTCTATTTGTAAATCTGTTCAATACAGGGATTTTGAACTATCTATGAAAAGCCA
  AAACTATTGGGAAATGTGTTCATTTAGTGAAACAGAGGCCAGCCGCATTGCAAATGAGTACCCAGAGGAT
  TTTGTTAATTATAATAAGAAGTTCTTATCAAGAATCTATCCAAGTGCCATGAGGATCGATTCCAGTAACT
  TGAATCCACAGGACTTTTGGAATTGTGGCTGTCAGATTGTAGCAATGAATTTTCAGACTCCGGGTCCAAT
  GATGGACCTTCACACGGGCTGGTTTCTTCAAAACGGGGGATGTGGTTATGTTCTAAGGCCGTCTATAATG
  CGAGATGAAGTTTCTTACTTCAGCGCAAATACAAAGGGCATTCTACCTGGGGTGTCTCCTCTAGCTCTTC
  ATATCAAGATCATCAGTGGTCAGAATTTCCCAAAGCCCAAGGGAGCTTGTGCCAAAGGGGATGTCATAGA
  TCCCTATGTTTGTATAGAGATACACGGAATTCCAGCGGATTGTTCGGAACAAAGAACTAAAACTGTACAG
  CAAAACAGTGATAATCCTATTTTTGATGAAACTTTTGAGTTCCAAGTAAACCTACCTGAGCTGGCCATGA
  TCCGTTTTGTTGTTCTGGATGATGACTACATTGGGGATGAGTTTATAGGGCAATATACGATACCATTTGA
  ATGTTTGCAGCCTGGATATCGGCATGTTCCCCTGCGTTCTTTTGTGGGTGACATCATGGAGCACGTAACC
  CTTTTTGTCCACATAGCAATAACTAATCGAAGTGGAGGAGGAAAGGCACAGAAGCGCAGTCTTTCAGTGA
  GAATGGGGAAGAAAGTTCGGGAATATACCATGCTCAGGAATATCGGTCTTAAAACCATTGATGACATCTT
  TAAAATAGCGGTTCATCCATTACGAGAAGCCATAGATATGAGAGAAAATATGCAGAATGCAATCGTGTCT
  ATTAAGGAACTATGTGGACTCCCTCCAATTGCCAGTCTGAAGCAGTGCCTGTTAACTCTGTCATCTCGGC
  TCATCACCAGTGACAATACTCCTTCAGTCTCACTTGTGATGAAAGACAGCTTTCCTTACCTGGAGCCTCT
  GGGTGCAATTCCAGATGTGCAGAAAAAGATGCTGACTGCTTATGATCTGATGATTCAAGAGAGCCGGTTT
  CTCATAGAAATGGCGGACACAGTCCAGGAAAAGATTGTACAGTGTCAGAAAGCAGGGATGGAGTTCCATG
  AAGAACTTCATAATTTGGGGGCAAAAGAAGGCTTGAAGGGAAGAAAACTCAACAAAGCAACTGAGAGCTT
  TGCTTGGAACATTACAGTATTGAAGGGCCAAGGAGATCTGTTGAAGAATGCCAAGAATGAAGCTATAGAA
  AACATGAAGCAGATCCAGCTGGCATGCCTGTCCTGTGGACTGAGTAAAGCCCCCAGCAGCAGTGCTGAGG
  CCAAGAGCAAGCGCAGCCTGGAAGCCATAGAGGAGAAGGAAAGTAGTGAGGAGAATGGGAAGCTGTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_006226
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006226.3, NP_006217.3
• RefSeq Size: 6608 bp
• RefSeq ORF: 3288 bp
• Locus ID: 5334
• Cytogenetics: 2q33.1
• Domains: C2, PI-PLC-X, PI-PLC-Y, PH
• Protein Families: Druggable Genome
• Gene Summary: ''
  Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a.