SIGLEC6 (NM_001177549) Human Untagged Clone

CAT#: SC328547

SIGLEC6 (untagged)-Human sialic acid binding Ig-like lectin 6 (SIGLEC6) transcript variant 6

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SIGLEC6
• Synonyms: CD33L; CD33L1; CD33L2; CD327; CDW327; OBBP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001177549, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCAGGGAGCCCAGGAAGCCTCCGCCTCAGAGATGCTACCGCTGCTGCTGCCCCTGCTGTGGGCAGGGG
  CCCTGGCTCAGGAGCGGAGATTCCAGCTGGAGGGGCCAGAGTCACTGACGGTGCAGGAGGGTCTGTGCGT
  CCTCGTACCCTGCAGATTGCCCACTACCCTTCCAGCCTCGTACTATGGTTATGGCTACTGGTTCCTGGAA
  GGGGCTGATGTTCCAGTGGCCACAAACGACCCAGACGAAGAAGTGCAGGAGGAGACCCGGGGCCGATTCC
  ACCTCCTCTGGGATCCCAGAAGGAAGAACTGCTCCCTGAGCATCAGAGATGCCCGGAGGAGGGACAATGC
  TGCATACTTCTTTCGGTTGAAGTCCAAATGGATGAAATACGGTTATACATCTTCCAAGCTCTCTGTGCGT
  GTGATGGGGACCCTGGAGTCTGGCCATCCCAGCAATCTGACCTGCTCTGTGCCCTGGGTCTGTGAGCAGG
  GGACGCCCCCCATCTTCTCCTGGATGTCAGCTGCCCCCACCTCCCTGGGCCCCAGGACCACCCAGTCCTC
  GGTGCTCACAATCACCCCACGGCCCCAGGACCACAGCACCAACCTCACCTGTCAGGTGACGTTCCCTGGA
  GCCGGTGTGACCATGGAGAGAACCATCCAGCTCAATGTCTCCTATGCTCCACAGAAAGTGGCCATCAGCA
  TCTTCCAAGGAAACAGCGCAGCCTTCAAAATCCTGCAAAACACCTCGTCCCTCCCTGTCCTGGAGGGCCA
  GGCTCTGCGGCTGCTCTGTGATGCTGACGGCAACCCCCCTGCACACCTGAGCTGGTTCCAGGGCTTCCCC
  GCCCTGAACGCCACCCCCATCTCCAATACCGGGGTCCTGGAGCTGCCTCAAGTAGGGTCTGCAGAAGAAG
  GAGATTTCACCTGCCGTGCTCAGCATCCTCTGGGCTCCCTGCAAATCTCTCTGAGTCTCTTTGTGCATTG
  GTCATCAGCACCAGTTCCAGACAGGCATAGTTTCAGACCACCCTGCTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001177549
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001177549.2, NP_001171020.1
• RefSeq Size: 3654 bp
• RefSeq ORF: 1029 bp
• Locus ID: 946
• Cytogenetics: 19q13.41
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Gene Summary: 'This gene encodes a member of the SIGLEC (sialic acid binding immunoglobulin-like lectin) family of proteins. The encoded transmembrane receptor binds sialyl-TN glycans and leptin. Placental expression of the encoded protein is upregulated in preeclampsia. [provided by RefSeq, Jul 2016]'
  Transcript Variant: This variant (6) uses an alternate, in-frame splice site and lacks two exons in the coding region, which results in a frameshift and an early stop codon, compared to variant 1. The encoded isoform (5) is shorter and has a distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.