FOXO4 (NM_001170931) Human Untagged Clone

CAT#: SC328743

FOXO4 (untagged)-Human forkhead box O4 (FOXO4) transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FOXO4
• Synonyms: AFX; AFX1; MLLT7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001170931, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGATCCGGGGAATGAGAATTCAGCCACAGAGGCTGCCGCGATCATAGACCTAGATCCCGACTTCGAAC
  CCCAGAGCCGTCCCCGCTCCTGCACCTGGCCCCTTCCCCGACCAGAGATCGCTAACCAGCCGTCCGAGCC
  GCCCGAGGTGGAGCCAGATCTGGGGGAAAAGGCCATTGAAAGCGCCCCGGAGAAGCGACTGACACTTGCC
  CAGATCTACGAGTGGATGGTCCGTACTGTACCCTACTTCAAGGACAAGGGTGACAGCAACAGCTCAGCAG
  GATGGAAGAACTCGATCCGCCACAACCTGTCCCTGCACAGCAAGTTCATCAAGGTTCACAACGAGGCCAC
  CGGCAAAAGCTCTTGGTGGATGCTGAACCCTGAGGGAGGCAAGAGCGGCAAAGCCCCCCGCCGCCGGGCC
  GCCTCCATGGATAGCAGCAGCAAGCTGCTCCGGGGCCGCAGTAAAGCCCCCAAGAAGAAACCATCTGTGC
  TGCCAGCTCCACCCGAAGGTGCCACTCCAACGAGCCCTGTCGGCCACTTTGCCAAGTGGTCAGGCAGCCC
  TTGCTCTCGAAACCGTGAAGAAGCCGATATGTGGACCACCTTCCGTCCACGAAGCAGTTCAAATGCCAGC
  AGTGTCAGCACCCGGCTGTCCCCCTTGAGGCCAGAGTCTGAGGTGCTGGCGGAGGAAATACCAGCTTCAG
  TCAGCAGTTATGCAGGGGGTGTCCCTCCCACCCTCAATGAAGGTCTAGAGCTGTTAGATGGGCTCAATCT
  CACCTCTTCCCATTCCCTGCTATCTCGGAGTGGTCTCTCTGGCTTCTCTTTGCAGCATCCTGGGGTTACC
  GGCCCCTTACACACCTACAGCAGCTCCCTTTTCAGCCCAGCAGAGGGGCCCCTGTCAGCAGGAGAAGGGT
  GCTTCTCCAGCTCCCAGGCTCTGGAGGCCCTGCTCACCTCTGATACGCCACCACCCCCTGCTGACGTCCT
  CATGACCCAGGTAGATCCCATTCTGTCCCAGGCTCCGACTCTTCTGTTGCTGGGGGGGCTTCCTTCCTCC
  AGTAAGCTGGCCACGGGCGTCGGCCTGTGTCCCAAGCCCCTAGAGGCTCCAGGCCCCAGCAGTCTGGTTC
  CCACCCTTTCTATGATAGCACCACCTCCAGTCATGGCAAGTGCCCCCATCCCCAAGGCTCTGGGGACTCC
  TGTGCTCACACCCCCTACTGAAGCTGCAAGCCAAGACAGAATGCCTCAGGATCTAGATCTTGATATGTAT
  ATGGAGAACCTGGAGTGTGACATGGATAACATCATCAGTGACCTCATGGATGAGGGCGAGGGACTGGACT
  TCAACTTTGAGCCAGATCCCTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001170931
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001170931.1, NP_001164402.1
• RefSeq Size: 3200 bp
• RefSeq ORF: 1353 bp
• Locus ID: 4303
• Cytogenetics: Xq13.1
• Protein Families: Transcription Factors
• Gene Summary: 'This gene encodes a member of the O class of winged helix/forkhead transcription factor family. Proteins encoded by this class are regulated by factors involved in growth and differentiation indicating they play a role in these processes. A translocation involving this gene on chromosome X and the homolog of the Drosophila trithorax gene, encoding a DNA binding protein, located on chromosome 11 is associated with leukemia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010]'
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region compared to variant 1. This results in a shorter protein (isoform 2 also known as isoform zeta) compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.