CDC45L (CDC45) (NM_001178011) Human Untagged Clone

CAT#: SC328857

CDC45 (untagged)-Human cell division cycle 45 homolog (S. cerevisiae) (CDC45) transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CDC45
• Synonyms: CDC45L; CDC45L2; MGORS7; PORC-PI-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001178011, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTTCGTGTCCGATTTCCGCAAAGAGTTCTACGAGGTGGTCCAGAGCCAGAGGGTCCTTCTCTTCGTGG
  CCTCGGACGTGGATGCTCTGTGTGCGTGCAAGATCCTTCAGGCCTTGTTCCAGTGTGACCACGTGCAATA
  TACGCTGGTTCCAGTTTCTGGGTGGCAAGAACTTGAAACTGCATTTCTTGAGCATAAAGAACAGATCAAA
  TTACTCATTAAACAAGATGATGACCTTGAAGTTCCCGCCTATGAAGACATCTTCAGGGATGAAGAGGAGG
  ATGAAGAGCATTCAGGAAATGACAGTGATGGGTCAGAGCCTTCTGAGAAGCGCACACGGTTAGAAGAGGA
  GATAGTGGAGCAAACCATGCGGAGGAGGCAGCGGCGAGAGTGGGAGGCCCGGAGAAGAGACATCCTCTTT
  GACTACGAGCAGTATGAATATCATGGGACATCGTCAGCCATGGTGATGTTTGAGCTGGCTTGGATGCTGT
  CCAAGGACCTGAATGACATGCTGTGGTGGGCCATCGTTGGACTAACAGACCAGTGGGTGCAAGACAAGAT
  CACTCAAATGAAATACGTGACTGATGTTGGTGTCCTGCAGCGCCACGTTTCCCGCCACAACCACCGGAAC
  GAGGATGAGGAGAACACACTCTCCGTGGACTGCACACGGATCTCCTTTGAGTATGACCTCCGCCTGGTGC
  TCTACCAGCACTGGTCCCTCCATGACAGCCTGTGCAACACCAGCTATACCGCAGCCAGGTTCAAGCTGTG
  GTCTGTGCATGGACAGAAGCGGCTCCAGGAGTTCCTTGCAGACATGGGTCTTCCCCTGAAGCAGGTGAAG
  CAGAAGTTCCAGGCCATGGACATCTCCTTGAAGGAGAATTTGCGGGAAATGATTGAAGAGTCTGCAAATA
  AATTTGGGATGAAGGACATGCGCGTGCAGACTTTCAGCATTCATTTTGGGTTCAAGCACAAGTTTCTGGC
  CAGCGACGTGGTCTTTGCCACCATGTCTTTGATGGAGAGCCCCGAGAAGGATGGCTCAGGGACAGATCAC
  TTCATCCAGGCTCTGGACAGCCTCTCCAGGAGTAACCTGGACAAGCTGTACCATGGCCTGGAACTCGCCA
  AGAAGCAGCTGCGAGCCACCCAGCAGACCATTGCCAGCTGCCTTTGCACCAACCTCGTCATCTCCCAGGG
  GCCTTTCCTGTACTGCTCTCTCATGGAGGGCACTCCAGATGTCATGCTGTTCTCTAGGCCGGCATCCCTA
  AGCCTGCTCAGCAAACACCTGCTCAAGTCCTTTGTGTGTTCGACAAAGAACCGGCGCTGCAAACTGCTGC
  CCCTGGTGATGGCTGCCCCCCTGAGCATGGAGCATGGCACAGTGACCGTGGTGGGCATCCCCCCAGAGAC
  CGACAGCTCGGACAGGAAGAACTTTTTTGGGAGGGCGTTTGAGAAGGCAGCGGAAAGCACCAGCTCCCGG
  ATGCTGCACAACCATTTTGACCTCTCAGTAATTGAGCTGAAAGCTGAGGATCGGAGCAAGTTTCTGGACG
  CACTTATTTCCCTCCTGTCCTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001178011
• ORF Size: 1563 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_001178011.2, NP_001171482.1
• RefSeq Size: 1860
• RefSeq ORF: 1563
• Locus ID: 8318
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Cell cycle
• Gene Summary: The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
  Transcript Variant: This variant (3) lacks two in-frame exons in the CDS, compared to variant 1. The resulting isoform (3) is shorter than isoform 1.