PPP2R1B (NM_001177562) Human Untagged Clone

CAT#: SC328903

PPP2R1B (untagged)-Human protein phosphatase 2 regulatory subunit A beta (PPP2R1B) transcript variant 4


  "NM_001177562" in other vectors (4)

Reconstitution Protocol

USD 950.00

3 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol PPP2R1B
Synonyms PP2A-Abeta; PR65B
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>NCBI ORF sequence for NM_001177562, the custom clone sequence may differ by one or more nucleotides


ATGGCGGGCGCATCAGAGCTCGGGACCGGCCCAGGAGCAGCGGGTGGAGATGGAGATGATTCGCTATACC
CGATCGCGGTTTTAATCGACGAGCTCCGCAATGAAGACGTGCAGCTCCGACTCAACAGTATTAAGAAGTT
ATCAACAATTGCCCTAGCACTTGGAGTAGAAAGGACCCGAAGTGAATTGTTGCCATTTCTTACAGATACA
ATTTATGATGAAGATGAGGTACTATTAGCTCTTGCTGAGCAGCTGGGAAATTTCACTGGCCTAGTGGGAG
GTCCTGACTTTGCCCACTGTCTGCTGCCTCCTTTGGAAAATCTGGCAACTGTGGAAGAGACTGTTGTTCG
TGACAAGGCTGTGGAGTCCCTGAGACAGATCTCCCAGGAGCATACTCCTGTTGCTCTGGAAGCTTATTTT
GTACCTCTGGTGAAACGCTTAGCAAGTGGGGATTGGTTCACCTCTCGCACATCTGCATGTGGTTTGTTCA
GCGTTTGCTATCCCAGGGCATCAAATGCTGTTAAAGCAGAAATCAGACAGCAATTCCGTTCCTTGTGCTC
AGATGACACACCAATGGTACGACGTGCTGCTGCTTCCAAATTGGGTGAATTTGCAAAAGTTTTGGAATTA
GACAGTGTGAAAAGTGAAATTGTTCCACTGTTCACTAGTCTAGCTTCAGATGAACAGGATTCAGTGCGCC
TCCTTGCTGTGGAAGCTTGTGTCAGTATTGCCCAGTTATTGTCTCAGGATGACCTTGAGACTTTGGTGAT
GCCTACACTTCGACAAGCAGCAGAAGATAAATCTTGGCGCGTTCGCTATATGGTGGCTGACAGATTTTCA
GAGCTCCAGAAAGCCATGGGTCCTAAAATCACCCTAAATGACCTCATCCCCGCCTTTCAGAACCTACTTA
AAGACTGTGAAGCTGAAGTCCGGGCAGCTGCTGCCCACAAAGTAAAAGAACTTGGTGAGAACTTGCCCAT
TGAAGATAGAGAGACCATAATTATGAATCAAATTCTGCCTTATATAAAGTGTCCTGACGTTCGTTTGAAT
ATCATCTCCAATTTGGATTGTGTAAATGAAGTGATTGGAATCCGTCAGCTCTCTCAGTCTCTCCTTCCTG
CCATAGTGGAGCTGGCAGAAGATGCCAAATGGAGGGTCCGCCTGGCCATCATTGAGTATATGCCGCTGCT
GGCAGGCCAGCTGGGTGTGGAATTCTTTGATGAAAAGCTGAATTCTTTATGTATGGCTTGGCTCGTGGAC
CATGTATACGCCATCCGAGAAGCTGCCACCAACAACCTCATGAAACTAGTTCAGAAGTTTGGTACAGAGT
GGGCCCAAAATACTATTGTTCCCAAAGTGTTAGTAATGGCAAATGATCCTAATTACTTGCATAGAATGAC
CACTTTATTCTGCATTAATGCACTGTCTGAGGCCTGTGGTCAGGAAATAACTACTAAGCAAATGCTGCCC
ATCGTATTAAAAATGGCAGGAGACCAAGTAGCAAATGTTCGCTTCAATGTGGCCAAATCTCTACAAAAGA
TTGGACCAATTCTAGATACCAATGCTTTACAGGGAGAAGTGAAGCCAGTACTACAGAAGTTAGGTCAAGA
TGAAGACATGGATGTCAAATACTTTGCACAGGAAGCTATAAGTGTTCTTGCATTGGCATAA


Restriction Sites SgfI-MluI     
ACCN NM_001177562
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001177562.1, NP_001171033.1
RefSeq Size 5470 bp
RefSeq ORF 1671 bp
Locus ID 5519
Cytogenetics 11q23.1
Protein Families Druggable Genome, Phosphatase, Transcription Factors
Protein Pathways Long-term depression, Oocyte meiosis, TGF-beta signaling pathway, Tight junction, Wnt signaling pathway
Gene Summary 'This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes a beta isoform of the constant regulatory subunit A. Mutations in this gene have been associated with some lung and colon cancers. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010]'
Transcript Variant: This variant (4) lacks an in-frame exon in the coding region compared to variant 1. The resulting isoform (d) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.