DMT1 (SLC11A2) (NM_001174128) Human Untagged Clone

CAT#: SC328914

SLC11A2 (untagged)-Human solute carrier family 11 (proton-coupled divalent metal ion transporters) member 2 (SLC11A2) transcript variant 5

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SLC11A2
• Synonyms: AHMIO1; DCT1; DMT1; NRAMP2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001174128, the custom clone sequence may differ by one or more nucleotides
  ATGGTGCTGGGTCCTGAACAGAAGATGTCAGATGACAGTGTTTCTGGAGATCATGGGGAG
  TCTGCCAGTCTTGGTAACATCAACCCTGCCTATAGTAATCCCTCTCTTTCACAGTCCCCT
  GGGGACTCAGAGGAGTACTTCGCCACTTACTTTAATGAGAAGATCTCCATTCCTGAGGAG
  GAGTACTCTTGTTTTAGCTTTCGTAAACTCTGGGCTTTCACCGGACCAGGTTTTCTTATG
  AGCATTGCCTACCTGGATCCAGGAAATATTGAATCCGATTTGCAGTCTGGAGCAGTGGCT
  GGATTTAAGTTGCTCTGGATCCTTCTGTTGGCCACCCTTGTGGGGCTGCTGCTCCAGCGG
  CTTGCAGCTAGACTGGGAGTGGTTACTGGGCTGCATCTTGCTGAAGTATGTCACCGTCAG
  TATCCCAAGGTCCCACGAGTCATCCTGTGGCTGATGGTGGAGTTGGCTATCATCGGCTCA
  GACATGCAAGAAGTCATTGGCTCAGCCATTGCTATCAATCTTCTGTCTGTAGGAAGAATT
  CCTCTGTGGGGTGGCGTTCTCATCACCATTGCAGATACTTTTGTATTTCTCTTCTTGGAC
  AAATATGGCTTGCGGAAGCTAGAAGCATTTTTTGGCTTTCTCATCACTATTATGGCCCTC
  ACATTTGGATATGAGTATGTTACAGTGAAACCCAGCCAGAGCCAGGTACTCAAGGGCATG
  TTCGTACCATCCTGTTCAGGCTGTCGCACTCCACAGATTGAACAGGCTGTGGGCATCGTG
  GGAGCTGTCATCATGCCACACAACATGTACCTGCATTCTGCCTTAGTCAAGTCTAGACAG
  GTAAACCGGAACAATAAGCAGGAAGTTCGAGAAGCCAATAAGTACTTTTTCATTGAATCC
  TGCATTGCACTCTTTGTTTCCTTCATCATCAATGTCTTTGTTGTCTCAGTCTTTGCTGAA
  GCATTTTTTGGGAAAACCAACGAGCAGGTGGTTGAAGTCTGTACAAATACCAGCAGTCCT
  CATGCTGGCCTCTTTCCTAAAGATAACTCGACACTGGCTGTGGACATCTACAAAGGGGGT
  GTTGTGCTGGGATGTTACTTTGGGCCTGCTGCACTCTACATTTGGGCAGTGGGGATCCTG
  GCTGCAGGACAGAGCTCCACCATGACAGGAACCTATTCTGGCCAGTTTGTCATGGAGGGA
  TTCCTGAACCTAAAGTGGTCACGCTTTGCCCGAGTGGTTCTGACTCGCTCTATTGCCATC
  ATCCCCACTCTGCTTGTTGCTGTCTTCCAAGATGTAGAGCATCTAACAGGGATGAATGAC
  TTTCTGAATGTTCTACAGAGCTTACAGCTTCCCTTTGCTCTCATACCCATCCTCACATTT
  ACGAGCTTGCGGCCAGTAATGAGTGACTTTGCCAATGGACTAGGCTGGCGGATTGCAGGA
  GGAATCTTGGTCCTTATCATCTGTTCCATCAATATGTACTTTGTAGTGGTTTATGTCCGG
  GACCTAGGGCATGTGGCATTATATGTGGTGGCTGCTGTGGTCAGCGTGGCTTATCTGGGC
  TTTGTGTTCTACTTGGGTTGGCAATGTTTGATTGCACTGGGCATGTCCTTCCTGGACTGT
  GGGCATACGGTAAGCATCTCTAAAGGCCTGCTGACAGAAGAAGCCACCCGTGGCTACGTT
  AAATAA
  
• Restriction Sites: Please inquire
• ACCN: NM_001174128
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001174128.1, NP_001167599.1
• RefSeq Size: 4159 bp
• RefSeq ORF: 1686 bp
• Locus ID: 4891
• Cytogenetics: 12q13.12
• Protein Families: Transmembrane
• Protein Pathways: Lysosome
• Gene Summary: 'This gene encodes a member of the solute carrier family 11 protein family. The product of this gene transports divalent metals and is involved in iron absorption. Mutations in this gene are associated with hypochromic microcytic anemia with iron overload. A related solute carrier family 11 protein gene is located on chromosome 2. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Apr 2010]'
  Transcript Variant: This variant (5) differs in the 5' UTR and 5' coding region compared to variant 1. The resulting protein (isoform 3) has a shorter and distinct N-terminus compared to isoform 1. Variants 4, 5 and 6 encode isoform 3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.