PGM1 (NM_001172818) Human Untagged Clone

CAT#: SC328946

PGM1 (untagged)-Human phosphoglucomutase 1 (PGM1) transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PGM1
• Synonyms: CDG1T; GSD14
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001172818, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGTGATTTTGAAGAATGGATTTCTGGGACATATAGAAAAATGGAAGAAGGTCCTCTCCCTCTGTTGA
  CTTTTGCTACAGCTCCCTACCACGATCAGAAACCAGGAACAAGTGGATTACGGAAGAAAACCTATTATTT
  TGAGGAAAAGCCATGCTATCTGGAGAATTTCATCCAGAGTATATTCTTTTCCATAGACCTAAAAGATCGC
  CAGGGATCATCACTGGTGGTTGGTGGAGATGGGCGGTACTTTAATAAATCAGCAATAGAAACAATAGTGC
  AGATGGCAGCTGCCAATGGGATCGGTCGCTTGGTTATCGGACAGAATGGAATCCTCTCCACCCCTGCTGT
  ATCCTGCATCATTAGAAAAATCAAAGCCATTGGTGGGATCATTCTGACAGCCAGTCACAACCCAGGGGGC
  CCCAATGGAGATTTTGGAATCAAATTCAATATTTCTAATGGAGGTCCTGCTCCAGAAGCAATAACTGATA
  AAATTTTCCAAATCAGCAAGACAATTGAAGAATATGCAGTTTGCCCTGACCTGAAAGTAGACCTTGGTGT
  TCTGGGAAAGCAGCAGTTTGACTTGGAAAATAAGTTCAAACCCTTCACAGTGGAAATTGTGGATTCGGTA
  GAAGCTTATGCTACAATGCTGAGAAGCATCTTTGATTTCAGTGCACTGAAAGAACTACTTTCTGGGCCAA
  ACCGACTGAAGATCCGTATTGATGCTATGCATGGAGTTGTGGGACCGTATGTAAAGAAGATCCTCTGTGA
  AGAACTCGGTGCCCCTGCGAACTCGGCAGTTAACTGCGTTCCTCTGGAGGACTTTGGAGGCCACCACCCT
  GACCCCAACCTCACCTATGCAGCTGACCTGGTGGAGACCATGAAGTCAGGAGAGCATGATTTTGGGGCTG
  CCTTTGATGGAGATGGGGATCGAAACATGATTCTGGGCAAGCATGGGTTCTTTGTGAACCCTTCAGACTC
  TGTGGCTGTCATTGCTGCCAACATCTTCAGCATTCCGTATTTCCAGCAGACTGGGGTCCGCGGCTTTGCA
  CGGAGCATGCCCACGAGTGGTGCTCTGGACCGGGTGGCTAGTGCTACAAAGATTGCTTTGTATGAGACCC
  CAACTGGCTGGAAGTTTTTTGGGAATTTGATGGACGCGAGCAAACTGTCCCTTTGTGGGGAGGAGAGCTT
  CGGGACCGGTTCTGACCACATCCGTGAGAAAGATGGACTGTGGGCTGTCCTTGCCTGGCTCTCCATCCTA
  GCCACCCGCAAGCAGAGTGTGGAGGACATTCTCAAAGATCATTGGCAAAAGTATGGCCGGAATTTCTTCA
  CCAGGTATGATTACGAGGAGGTGGAAGCTGAGGGCGCAAACAAAATGATGAAGGACTTGGAGGCCCTGAT
  GTTTGATCGCTCCTTTGTGGGGAAGCAGTTCTCAGCAAATGACAAAGTTTACACTGTGGAGAAGGCCGAT
  AACTTTGAATACAGCGACCCAGTGGATGGAAGCATTTCAAGAAATCAGGGCTTGCGCCTCATTTTCACAG
  ATGGTTCTCGAATCGTCTTCCGACTGAGCGGCACTGGGAGTGCCGGGGCCACCATTCGGCTGTACATCGA
  TAGCTATGAGAAGGACGTTGCCAAGATTAACCAGGACCCCCAGGTCATGTTGGCCCCCCTTATTTCCATT
  GCTCTGAAAGTGTCCCAGCTGCAGGAGAGGACGGGACGCACTGCACCCACTGTCATCACCTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001172818
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001172818.1, NP_001166289.1
• RefSeq Size: 2573 bp
• RefSeq ORF: 1743 bp
• Locus ID: 5236
• Cytogenetics: 1p31.3
• Protein Pathways: Amino sugar and nucleotide sugar metabolism, Galactose metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Pentose phosphate pathway, Starch and sucrose metabolism
• Gene Summary: 'The protein encoded by this gene is an isozyme of phosphoglucomutase (PGM) and belongs to the phosphohexose mutase family. There are several PGM isozymes, which are encoded by different genes and catalyze the transfer of phosphate between the 1 and 6 positions of glucose. In most cell types, this PGM isozyme is predominant, representing about 90% of total PGM activity. In red cells, PGM2 is a major isozyme. This gene is highly polymorphic. Mutations in this gene cause glycogen storage disease type 14. Alternativley spliced transcript variants encoding different isoforms have been identified in this gene.[provided by RefSeq, Mar 2010]'
  Transcript Variant: This variant (2) has an alternate 5' exon, as compared to variant 1. The resulting isoform (2) has a longer and different N-terminus, as compared to isoform 1.