EHHADH (NM_001166415) Human Untagged Clone

CAT#: SC329004

EHHADH (untagged)-Human enoyl-CoA hydratase/3-hydroxyacyl CoA dehydrogenase (EHHADH) transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EHHADH
• Synonyms: ECHD; FRTS3; L-PBE; LBFP; LBP; PBFE
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001166415, the custom clone sequence may differ by one or more nucleotides
  ATGGCTTTCGGAGGGGGACTAGAGCTGGCCCTGGGCTGTCACTATAGGATTGCCCACGCA
  GAGGCTCAAGTTGGCTTACCAGAAGTTACACTGGGACTTCTCCCTGGTGCAAGAGGAACC
  CAGCTTCTCCCCAGACTCACTGGAGTTCCTGCTGCACTTGACTTAATTACCTCAGGAAGA
  CGTATTTTAGCAGATGAAGCACTCAAGCTGGGCATTCTAGATAAAGTTGTAAACTCAGAC
  CCGGTTGAAGAAGCAATCAGATTTGCTCAGAGAGTTTCAGATCAACCTCTAGAATCCCGT
  AGACTCTGCAACAAGCCAATTCAGAGCTTGCCCAACATGGACAGCATTTTTAGTGAGGCC
  CTCTTGAAGATGCGGAGGCAGCACCCTGGGTGTCTTGCACAGGAGGCTTGTGTCCGTGCA
  GTCCAGGCTGCTGTGCAGTATCCCTATGAAGTGGGCATCAAGAAGGAGGAGGAGCTGTTT
  CTATATCTTTTGCAATCAGGGCAGGCTAGAGCCCTGCAATATGCTTTCTTCGCTGAAAGG
  AAAGCAAATAAGTGGTCAACTCCCTCCGGAGCATCGTGGAAAACAGCATCAGCGCGGCCT
  GTCTCCTCAGTTGGTGTTGTTGGCTTGGGAACAATGGGCCGAGGCATTGTCATTTCTTTT
  GCAAGGGCCAGGATTCCTGTGATTGCTGTAGACTCGGACAAAAACCAGCTAGCAACTGCA
  AACAAGATGATAACCTCTGTCTTGGAAAAAGAAGCCTCCAAAATGCAACAGAGCGGCCAC
  CCTTGGTCAGGACCAAAACCCAGGTTAACTTCATCTGTGAAGGAGCTTGGTGGTGTAGAT
  TTAGTCATTGAAGCAGTATTTGAGGAAATGAGCCTGAAGAAGCAGGTCTTTGCTGAACTC
  TCAGCTGTGTGCAAACCAGAAGCATTTTTGTGCACTAATACTTCAGCCCTGGATGTTGAT
  GAGATTGCTTCTTCCACTGATCGTCCTCACTTGGTCATTGGCACCCACTTCTTTTCGCCA
  GCTCATGTCATGAAGTTGTTAGAGGTTATTCCCAGCCAATACTCTTCCCCCACTACCATT
  GCCACTGTTATGAACTTATCAAAAAAGATTAAAAAGATTGGAGTCGTTGTAGGCAACTGT
  TTTGGATTTGTGGGGAATCGAATGTTGAATCCTTACTACAATCAGGCATATTTCTTGTTA
  GAAGAAGGCAGCAAACCAGAGGAGGTAGATCAGGTGCTGGAAGAGTTTGGTTTTAAAATG
  GGACCTTTTAGAGTGTCTGATCTTGCTGGGTTGGATGTGGGCTGGAAATCTAGAAAGGGG
  CAAGGTCTTACTGGACCTACATTGCTTCCAGGAACTCCTGCCCGAAAAAGGGGTAATAGG
  AGGTACTGCCCAATTCCTGATGTGCTCTGTGAATTAGGACGATTTGGCCAGAAGACAGGT
  AAGGGTTGGTATCAATATGACAAGCCATTGGGTAGGATTCACAAACCTGATCCCTGGCTT
  TCCAAATTCCTATCACGGTATAGAAAAACCCATCACATTGAACCACGTACCATTAGCCAG
  GATGAGATCCTTGAACGCTGCTTATATTCACTTATCAATGAAGCATTCCGTATCTTGGGA
  GAAGGGATAGCTGCTAGCCCAGAGCACATTGATGTTGTCTATTTACATGGATATGGATGG
  CCAAGGCACAAGGGCGGGCCCATGTTCTATGCTTCCACAGTTGGGTTGCCCACAGTTCTA
  GAGAAATTGCAGAAATATTACAGGCAGAACCCTGATATTCCCCAACTGGAGCCAAGTGAC
  TATCTAAAAAAACTGGCTTCTCAGGGAAACCCTCCCCTGAAAGAATGGCAAAGCTTGGCA
  GGCTCCCCTAGCAGTAAATTGTGA
  
• Restriction Sites: Please inquire
• ACCN: NM_001166415
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001166415.1, NP_001159887.1
• RefSeq Size: 3993 bp
• RefSeq ORF: 1884 bp
• Locus ID: 1962
• Cytogenetics: 3q27.2
• Protein Pathways: beta-Alanine metabolism, Butanoate metabolism, Fatty acid metabolism, Limonene and pinene degradation, Lysine degradation, Metabolic pathways, PPAR signaling pathway, Propanoate metabolism, Tryptophan metabolism, Valine, leucine and isoleucine degradation
• Gene Summary: 'The protein encoded by this gene is a bifunctional enzyme and is one of the four enzymes of the peroxisomal beta-oxidation pathway. The N-terminal region of the encoded protein contains enoyl-CoA hydratase activity while the C-terminal region contains 3-hydroxyacyl-CoA dehydrogenase activity. Defects in this gene are a cause of peroxisomal disorders such as Zellweger syndrome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]'
  Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1.