PLS1 (NM_001172312) Human Untagged Clone

CAT#: SC329007

PLS1 (untagged)-Human plastin 1 (PLS1) transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PLS1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001172312, the custom clone sequence may differ by one or more nucleotides
  ATGGAAAACAGTACTACTACCATTTCTCGGGAGGAGCTTGAAGAACTACAAGAGGCATTT
  AATAAAATAGATATTGACAATAGTGGGTATGTCAGTGACTATGAACTTCAAGACCTGTTT
  AAGGAAGCAAGCCTTCCTCTGCCTGGCTACAAGGTGCGCGAGATTGTGGAGAAAATTCTA
  TCAGTTGCTGACAGCAACAAAGATGGCAAAATCAGTTTTGAAGAGTTTGTGTCACTAATG
  CAAGAATTAAAAAGCAAAGATATCAGCAAAACATTCCGAAAAATAATTAACAAGAGGGAA
  GGGATTACTGCTATTGGAGGAACTTCAACTATTTCCAGTGAGGGCACACAGCATTCTTAT
  TCAGAGGAAGAAAAAGTGGCTTTTGTTAACTGGATAAACAAAGCCCTGGAGAATGACCCT
  GACTGTAAGCATCTTATACCCATGAATCCCAATGATGATAGTCTTTTCAAGTCACTTGCA
  GATGGCATCCTTCTTTGCAAAATGATCAACTTATCTGAACCAGATACAATTGATGAAAGA
  GCCATCAATAAGAAAAAGCTCACGCCATTCACTATTTCTGAAAATTTAAACCTAGCTCTG
  AATTCTGCCTCAGCCATTGGTTGTACAGTGGTCAACATTGGTGCATCAGATCTCAAAGAA
  GGAAAACCTCACTTGGTCTTGGGACTTCTCTGGCAGATCATCAAAGTTGGCCTTTTTGCT
  GATATTGAGATTTCCAGGAATGAAGCTCTGATTGCATTGTTAAATGAAGGTGAGGAACTA
  GAGGAGCTGATGAAGCTTTCTCCCGAGGAATTACTGCTGCGATGGGTGAACTACCATCTG
  ACCAATGCAGGATGGCATACCATCAGCAACTTCAGCCAAGACATTAAGGACTCGAGAGCC
  TATTTTCATCTGCTTAATCAGATTGCCCCTAAAGGTGGGGAAGATGGACCTGCCATTGCC
  ATTGACCTTTCAGGAATTAATGAGACAAATGACCTGAAGCGTGCTGGACTCATGCTTCAA
  GAAGCAGATAAACTGGGCTGCAAACAGTTTGTTACTCCTGCAGATGTGGTTTCAGGCAAT
  CCTAAACTTAATTTAGCTTTTGTAGCTAATTTGTTTAACACATACCCGTGCCTGCACAAG
  CCGAATAATAATGACATCGATATGAATTTACTGGAAGGAGAGAGCAAGGAAGAGAGAACA
  TTTCGGAACTGGATGAATTCCTTGGGAGTCAACCCATACATTAATCATTTGTACAGTGAC
  CTTGCAGATGCTTTAGTGATCTTTCAGCTCTATGAGATGATCCGAGTGCCAGTCAACTGG
  AGCCATGTCAACAAACCTCCTTATCCTGCCCTTGGAGGGAACATGAAGAAGATTGAAAAC
  TGTAACTATGCAGTGGAACTTGGGAAGAACAAGGCCAAATTCTCCTTGGTTGGCATTGCT
  GGGCAGGACCTAAATGAAGGGAATTCAACACTTACCCTGGCATTGGTATGGCAGCTGATG
  AGAAGGTACACATTGAATGTGTTATCGGATCTTGGAGAGGGTGAAAAAGTAAATGATGAA
  ATTATAATTAAATGGGTCAATCAGACTCTTAAAAGTGCAAACAAAAAGACTTCTATTTCC
  AGCTTCAAGGATAAATCTATAAGCACAAGTTTACCTGTCCTAGATTTAATAGATGCCATT
  GCACCAAATGCAGTTCGTCAAGAAATGATCAGGAGAGAAAACTTATCTGATGAGGACAAG
  CTGAACAATGCTAAATACGCCATTTCAGTTGCTCGAAAGATCGGTGCCCGGATATATGCA
  TTACCTGATGACCTCGTAGAAGTGAAACCAAAGATGGTTATGACGGTGTTTGCATGCTTA
  ATGGGAAAAGGACTGAACAGAATAAAATAA
  
• Restriction Sites: Please inquire
• ACCN: NM_001172312
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001172312.1, NP_001165783.1
• RefSeq Size: 3880 bp
• RefSeq ORF: 1890 bp
• Locus ID: 5357
• Cytogenetics: 3q23
• Gene Summary: 'Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. The protein encoded by this gene is a third distinct plastin isoform, which is specifically expressed at high levels in the small intestine. Alternatively spliced transcript variants varying in the 5' UTR, but encoding the same protein, have been found for this gene. A pseudogene of this gene is found on chromosome 11.[provided by RefSeq, Feb 2010]'
  Transcript Variant: This variant (3) contains an alternate 5' terminal non-coding exon, thus has a different 5' UTR compared to variant 1. Transcript variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.