AMPD1 (NM_001172626) Human Untagged Clone

CAT#: SC329127

AMPD1 (untagged)-Human adenosine monophosphate deaminase 1 (AMPD1) transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AMPD1
• Synonyms: MAD; MADA; MMDD
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001172626, the custom clone sequence may differ by one or more nucleotides
  ATGAATGTCAGGATATTTTATAGTGTCAGTCAGTCACCCCACAGTCTCCTCTCTCTTCTT
  TTCTACTGTGCTATCCTAGAATCAAGGATTTCAGCAACAATGCCTCTGTTCAAACTCCCA
  GAAATTGATGATGCAATGCGCAACTTTGCTGAAAAAGTGTTTGCCTCTGAAGTCAAAGAT
  GAAGGAGGTCGTCAGGAGATTTCCCCCTTTGATGTGGATGAGATCTGTCCGATTTCTCAT
  CATGAGATGCAAGCACACATATTCCATCTGGAGACTCTGTCCACCTCCACAGAAGCCAGG
  AGAAAAAAGCGTTTCCAAGGACGGAAGACTGTTAATTTGTCCATTCCACTAAGTGAAACA
  TCTTCCACCAAACTGTCCCACATTGATGAATACATTTCCTCATCTCCAACCTACCAGACC
  GTGCCTGATTTTCAGAGAGTGCAGATTACTGGTGACTATGCCTCTGGGGTTACAGTTGAA
  GATTTTGAAATTGTTTGCAAAGGTCTGTATCGGGCACTATGCATACGTGAGAAATACATG
  CAGAAGTCGTTTCAGAGGTTCCCTAAAACCCCTTCCAAATACTTGCGGAACATTGATGGT
  GAGGCTTGGGTAGCAAATGAGAGCTTCTATCCAGTCTTTACTCCTCCTGTGAAGAAGGGA
  GAGGACCCCTTCCGAACAGACAACCTTCCTGAAAACCTGGGCTATCACCTCAAAATGAAG
  GACGGTGTAGTTTACGTCTATCCTAATGAAGCAGCAGTCAGCAAAGATGAGCCTAAGCCA
  CTTCCTTACCCAAATCTGGACACCTTCTTAGACGATATGAATTTTTTACTTGCTTTAATT
  GCTCAAGGACCTGTTAAGACCTATACCCACCGGCGCCTGAAGTTCCTCTCCTCCAAGTTC
  CAGGTCCATCAGATGCTTAACGAGATGGACGAGTTAAAGGAGCTGAAAAACAACCCCCAC
  CGAGATTTTTATAACTGCAGGAAGGTGGACACCCATATCCATGCAGCCGCTTGCATGAAC
  CAGAAACATCTGCTGCGTTTTATTAAGAAATCTTACCAAATTGATGCTGACAGAGTGGTC
  TATAGCACCAAAGAGAAGAATCTGACCCTAAAGGAACTTTTTGCTAAATTAAAAATGCAT
  CCTTATGACCTGACTGTTGATTCTCTGGATGTTCATGCTGGACGCCAGACCTTCCAGCGT
  TTTGATAAGTTCAATGACAAATATAATCCTGTAGGAGCAAGTGAGCTACGGGACCTCTAC
  TTGAAGACAGACAATTACATTAATGGGGAATATTTTGCCACTATCATCAAGGAGGTAGGT
  GCGGACCTGGTGGAGGCCAAGTACCAGCATGCTGAGCCCCGCCTGTCCATCTATGGCCGC
  AGTCCTGATGAGTGGAGCAAACTCTCCTCCTGGTTCGTCTGCAATCGCATCCACTGCCCC
  AACATGACATGGATGATCCAGGTTCCCAGGATCTATGATGTGTTCCGTTCCAAGAATTTC
  CTTCCACATTTTGGAAAAATGCTGGAGAATATTTTCATGCCAGTGTTTGAGGCCACCATC
  AACCCCCAGGCTGACCCAGAACTCAGTGTCTTCCTCAAGCATATCACTGGCTTTGACAGT
  GTGGATGATGAGTCCAAACACAGTGGCCACATGTTCTCCTCCAAGAGTCCCAAGCCCCAG
  GAGTGGACATTGGAAAAGAATCCATCTTACACTTACTATGCCTACTACATGTATGCAAAC
  ATCATGGTGCTCAACAGCCTGAGAAAGGAACGAGGCATGAATACGTTTCTGTTCCGACCT
  CACTGTGGAGAAGCTGGAGCCCTCACCCATCTCATGACAGCATTCATGATAGCAGATGAT
  ATCTCTCATGGCCTAAATTTAAAAAAGAGTCCCGTGCTACAGTACTTGTTTTTCTTAGCC
  CAAATTCCCATCGCCATGTCACCACTAAGTAACAATAGCCTATTTCTAGAGTATGCCAAA
  AATCCTTTTTTGGATTTCCTTCAGAAAGGGCTAATGATCTCACTGTCTACAGATGACCCA
  ATGCAATTCCACTTTACCAAGGAGCCCCTAATGGAAGAATATGCTATTGCTGCACAAGTC
  TTCAAGCTGAGCACCTGTGATATGTGCGAAGTGGCAAGGAACAGTGTCTTGCAGTGTGGA
  ATTTCTCATGAGGAGAAAGTAAAGTTTCTGGGCGACAATTACCTTGAGGAAGGCCCTGCT
  GGAAATGATATCCGGAGGACAAATGTAGCCCAAATCCGCATGGCCTATCGCTATGAAACC
  TGGTGTTATGAACTCAATTTAATTGCTGAGGGTCTTAAATCAACAGAATAA
  
• Restriction Sites: Please inquire
• ACCN: NM_001172626
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001172626.1, NP_001166097.1
• RefSeq Size: 2414 bp
• RefSeq ORF: 2331 bp
• Locus ID: 270
• Cytogenetics: 1p13.2
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Purine metabolism
• Gene Summary: 'Adenosine monophosphate deaminase 1 catalyzes the deamination of AMP to IMP in skeletal muscle and plays an important role in the purine nucleotide cycle. Two other genes have been identified, AMPD2 and AMPD3, for the liver- and erythocyte-specific isoforms, respectively. Deficiency of the muscle-specific enzyme is apparently a common cause of exercise-induced myopathy and probably the most common cause of metabolic myopathy in the human. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene.[provided by RefSeq, Feb 2010]'
  Transcript Variant: This variant (2) lacks an in-frame coding exon in the 5' region, as compared to variant 1. The resulting isoform (2) is shorter than isoform 1.