MCF2 (NM_001171878) Human Untagged Clone

CAT#: SC329187

MCF2 (untagged)-Human MCF.2 cell line derived transforming sequence (MCF2) transcript variant 5

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MCF2
• Synonyms: ARHGEF21; DBL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001171878, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCAGAAGCAAATCCCCGGAGAGGCAAGATGAGGTTCAGAAGGAATGCGGCTTCCTTCCCTGGGAACT
  TGCACTTGGTTTTGGTTTTACGTCCTACCAGCTTTCTTCAACGAACGTTCACAGACATTGGATTTTGGTT
  TAGTCAGGAGGATTTTATGCTTAAATTACCAGTTGTTATGCTGAGCTCAGTTAGTGATTTGCTGACATAC
  ATTGATGACAAGCAATTAACCCCTGAGTTAGGCGGCACCTTGCAGTACTGCCACAGTGAATGGATCATCT
  TCAGAAATGCTATAGAAAATTTTGCCCTCACAGTGAAAGAAATGGCTCAGATGTTACAGTCCTTTGGAAC
  TGAACTGGCTGAGACAGAACTACCAGATGATATTCCCTCAATAGAAGAAATTCTGGCAATTCGTGCTGAA
  AGGTATCATCTGTTGAAGAATGATATTACAGCTGTAACCAAAGAAGGAAAAATTCTGCTAACAAATCTGG
  AAGTGCCTGACACTGAAGGAGCTGTCAGTTCAAGACTAGAATGTCATCGGCAAATAAGTGGTGACTGGCA
  AACTATTAATAAGTTGCTGACTCAAGTACATGATATGGAAACAGCTTTTGATGGATTTTGGGAAAAACAT
  CAATTAAAAATGGAGCAGTATCTGCAACTATGGAAGTTTGAGCAGGATTTTCAACAGCTTGTGACTGAAG
  TTGAATTTCTATTAAACCAACAAGCAGAACTGGCTGATGTAACAGGGACTATAGCTCAAGTAAAACAAAA
  AATAAAAAAATTGGAAAACTTAGATGAAAATTCTCAGGAGCTATTATCAAAGGCCCAGTTTGTGATATTA
  CATGGACACAAGCTTGCAGCAAATCACCATTATGCACTTGATTTAATCTGCCAGAGGTGCAATGAGCTAC
  GTTACCTTTCTGATATTTTGGTTAATGAGATAAAAGCAAAACGGATACAACTCAGCAGGACCTTCAAAAT
  GCATAAACTCCTACAGCAGGCTCGTCAATGCTGTGATGAAGGGGAATGTCTTCTAGCTAATCAGGAAATA
  GATAAGTTTCAGTCTAAAGAAGATGCTCAGAAAGCTCTCCAAGACATTGAAAATTTTCTTGAAATGGCTC
  TACCCTTTATAAATTATGAACCTGAAACACTGCAGTATGAATTTGATGTAATATTATCTCCTGAGCTTAA
  GGTTCAAATGAAGACTATACAACTCAAGCTTGAAAACATTCGAAGTATATTTGAGAACCAGCAGGCTGGT
  TTCAGGAACCTGGCAGATAAGCATGTGAGGCCAATCCAATTTGTGGTACCCACACCTGAAAATTTGGTCA
  CATCTGGGACACCATTTTTTTCATCTAAACAAGGGAAGAAGACTTGGAGACAAAATCAGAGCAACTTAAA
  AATTGAAGTGGTGCCTGATTGTCAGGAGAAGAGAAGTTCTGGTCCATCCTCCAGTTTGGACAATGGCAAT
  AGCTTGGATGTTTTAAAGAACCACGTACTAAATGAACTGATACAGACTGAGAGAGTTTATGTTCGAGAAC
  TGTATACTGTTTTGTTGGGTTATAGAGCGGAGATGGATAATCCAGAGATGTTTGATCTTATGCCACCTCT
  CCTGAGAAATAAAAAGGACATTCTCTTTGGAAACATGGCAGAAATATATGAATTCCATAACGACATTTTC
  TTGAGCAGCCTGGAAAATTGTGCTCATGCTCCAGAAAGAGTGGGACCTTGTTTCCTGGAAAGGAAGGATG
  ATTTTCAGATGTATGCAAAATATTGTCAGAATAAGCCCAGATCAGAAACAATTTGGAGGAAGTATTCAGA
  ATGCGCATTTTTCCAGGAATGTCAAAGAAAGTTAAAACACAGACTTAGACTGGATTCCTATTTACTCAAA
  CCAGTGCAACGAATCACTAAATATCAGTTATTGTTGAAGGAGCTATTAAAATATAGCAAAGACTGTGAAG
  GATCTGCTCTGTTGAAGAAGGCACTCGATGCAATGCTGGATTTACTGAAGTCAGTTAATGATTCTATGCA
  TCAGATTGCAATAAATGGCTATATTGGAAACTTAAATGAACTGGGCAAGATGATAATGCAAGGTGGATTC
  AGCGTTTGGATAGGGCACAAGAAAGGTGCTACAAAAATGAAGGATTTGGCTAGATTCAAACCAATGCAGC
  GACACCTTTTCTTGTATGAAAAAGCCATTGTTTTTTGCAAAAGGCGTGTTGAAAGTGGAGAAGGCTCTGA
  CAGATACCCGTCATACAGTTTTAAACACTGTTGGAAAATGGATGAAGTTGGAATCACTGAATATGTAAAA
  GGTGATAACCGCAAGTTTGAAATCTGGTATGGTGAAAAGGAAGAAGTTTATATTGTCCAGGCTTCTAATG
  TAGATGTGAAGATGACGTGGCTAAAAGAAATAAGAAATATTTTGTTGAAGCAGCAGGAACTTTTGACAGT
  TAAAAAAAGAAAGCAACAGGATCAATTAACAGAACGGGATAAGTTTCAGATTTCTCTTCAGCAGAATGAT
  GAAGACCTGTGTCGGAGATGGCTCTCCTATATTGATGAAGCTACTATGTCAAATGGCAAGTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001171878
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001171878.1, NP_001165349.1
• RefSeq Size: 3578 bp
• RefSeq ORF: 2583 bp
• Locus ID: 4168
• Cytogenetics: Xq27.1
• Protein Families: Druggable Genome
• Gene Summary: 'The oncogenic protein encoded by this gene is a guanine nucleotide exchange factor (GEF) that exerts control over some members of the Rho family of small GTPases. Several transcript variants encoding different isoforms have been found for this gene. These isoforms exhibit different expression patterns and varying levels of GEF activity.[provided by RefSeq, Jan 2010]'
  Transcript Variant: This variant (5) differs in the 5' UTR and coding sequence and lacks several exons compared to variant 3. The resulting isoform (e) has shorter and distinct N- and C-termini and lacks a segment compared to isoform c.