DAP5 (EIF4G2) (NM_001172705) Human Untagged Clone

CAT#: SC329218

EIF4G2 (untagged)-Human eukaryotic translation initiation factor 4 gamma 2 (EIF4G2) transcript variant 3


  "NM_001172705" in other vectors (4)

Reconstitution Protocol

USD 760.00

5 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol EIF4G2
Synonyms AAG1; DAP5; NAT1; P97
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_001172705, the custom clone sequence may differ by one or more nucleotides
GTGGAGAGTGCGATTGCAGAAGGGGGTGCTTCTCGTTTCAGTGCTTCTTCGGGCGGAGGA
GGAAGTAGGGGTGCACCTCAGCACTATCCCAAGACTGCTGGCAACAGCGAGTTCCTGGGG
AAAACCCCAGGGCAAAACGCTCAGAAATGGATTCCTGCACGAAGCACTAGACGAGATGAC
AACTCCGCAGCAAACAACTCCGCAAACGAAAAAGAACGACATGATGCAATCTTCAGGAAA
GTAAGAGGCATACTAAATAAGCTTACTCCTGAAAAGTTTGACAAGCTATGCCTTGAGCTC
CTCAATGTGGGTGTAGAGTCTAAACTCATCCTTAAAGGGGTCATACTGCTGATTGTGGAC
AAAGCCCTAGAAGAGCCAAAGTATAGCTCACTGTATGCTCAGCTATGTCTGCGATTGGCA
GAAGATGCACCAAACTTTGATGGCCCAGCAGCAGAGGGTCAACCAGGACAGAAGCAAAGC
ACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAATTTGAAAACCGAACTAGA
AATGTTGATGTCTATGATAAGCGTGAAAATCCCCTCCTCCCCGAGGAGGAGGAACAGAGA
GCCATTGCTAAGATCAAGATGTTGGGAAACATCAAATTCATTGGAGAGCTTGGCAAGCTT
GATCTTATTCACGAATCTATCCTTCATAAGTGCATCAAAACACTTTTGGAAAAGAAGAAG
AGAGTCCAACTCAAAGATATGGGAGAGGATTTGGAGTGCCTCTGTCAGATAATGAGGACA
GTGGGACCTAGATTAGACCATGAACGAGCCAAGTCCTTAATGGATCAGTACTTTGCCCGA
ATGTGCTCCTTGATGTTAAGTAAGGAATTGCCAGCAAGGATTCGTTTCCTGCTGCAGGAT
ACCGTAGAGTTGCGAGAACACCATTGGGTTCCTCGCAAGGCTTTTCTTGACAATGGACCA
AAGACGATCAATCAAATTCGTCAAGATGCAGTAAAAGATCTAGGGGTGTTTATTCCTGCT
CCTATGGCTCAAGGGATGAGAAGTGACTTCTTTCTGGAGGGACCGTTCATGCCACCCAGG
ATGAAAATGGATAGGGACCCACTTGGAGGACTTGCTGATATGTTTGGACAAATGCCAGGT
AGCGGAATTGGTACTGGTCCAGGAGTTATCCAGGATAGATTTTCACCCACCATGGGACGT
CATCGTTCAAATCAACTCTTCAATGGCCATGGGGGACACATCATGCCTCCCACACAATCG
CAGTTTGGAGAGATGGGAGGCAAGTTTATGAAAAGCCAGGGGCTAAGCCAGCTCTACCAT
AACCAGAGTCAGGGACTCTTATCCCAGCTGCAAGGACAGTCGAAGGATATGCCACCTCGG
TTTTCTAAGAAAGGACAGCTTAATGCAGATGAGATTAGCCTGAGGCCTGCTCAGTCGTTC
CTAATGAATAAAAATCAAGTGCCAAAGCTTCAGCCCCAGATAACTATGATTCCTCCTAGT
GCACAACCACCACGCACTCAAACACCACCTCTGGGACAGACACCTCAGCTTGGTCTCAAA
ACTAATCCACCGCTTATCCAGGAAAAGCCTGCCAAGACCAGCAAAAAGCCACCACCGTCA
AAGGAAGAACTCCTTAAACTAACTGAAACTGTTGTGACTGAATATCTAAATAGTGGAAAT
GCAAATGAGGCTGTCAATGGTGTAAGAGAAATGAGGGCTCCTAAACACTTTCTTCCTGAG
ATGTTAAGCAAAGTAATCATCCTGTCACTAGATAGAAGCGATGAAGATAAAGAAAAAGCA
AGTTCTTTGATCAGTTTACTCAAACAGGAAGGGATAGCCACAAGTGACAACTTCATGCAG
GCTTTCCTGAATGTATTGGACCAGTGTCCCAAACTGGAGGTTGACATCCCTTTGGTGAAA
TCCTATTTAGCACAGTTTGCAGCTCGTGCCATCATTTCAGAGCTGGTGAGCATTTCAGAA
CTAGCTCAACCACTAGAAAGTGGCACCCATTTTCCTCTCTTCCTACTTTGTCTTCAGCAG
TTAGCTAAATTACAAGATCGAGAATGGTTAACAGAACTTTTTCAACAAAGCAAGGTCAAT
ATGCAGAAAATGCTCCCAGAAATTGATCAGAATAAGGACCGCATGTTGGAGATTTTGGAA
GGAAAGGGACTGAGTTTCTTATTCCCACTCCTCAAATTGGAGAAGGAACTGTTGAAGCAA
ATAAAGTTGGATCCATCCCCTCAAACCATATATAAATGGATTAAAGATAACATCTCTCCC
AAACTTCATGTAGATAAAGGATTTGTGAACATCTTAATGACTAGCTTCTTACAGTACATT
TCTAGTGAAGTAAACCCCCCCAGCGATGAAACAGATTCATCCTCTGCTCCTTCCAAAGAA
CAGTTAGAGCAGGAAAAACAACTACTACTATCTTTCAAGCCAGTAATGCAGAAATTTCTT
CATGATCACGTTGATCTACAAGTCAGTGCCCTGTATGCTCTCCAGGTGCACTGCTATAAC
AGCAACTTCCCAAAAGGCATGTTACTTCGCTTTTTTGTGCACTTCTATGACATGGAAATT
ATTGAAGAAGAAGCTTTCTTGGCTTGGAAAGAAGATATAACCCAAGAGTTTCCGGGAAAA
GGCAAGGCTTTGTTCCAGGTGAATCAGTGGCTAACCTGGTTAGAAACTGCTGAAGAAGAA
GAATCAGAGGAAGAAGCTGACTAA
Restriction Sites Please inquire     
ACCN NM_001172705
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001172705.1, NP_001166176.1
RefSeq Size 4028 bp
RefSeq ORF 2724 bp
Locus ID 1982
Cytogenetics 11p15.4
Protein Families Transcription Factors
Protein Pathways Viral myocarditis
Gene Summary 'Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3; eIF4G, in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes. In vitro and in vivo studies indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq, Jul 2008]'
Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Both variants 1 and 3 encode the same isoform (1).

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.