MAN1 (LEMD3) (NM_001167614) Human Untagged Clone

CAT#: SC329219

LEMD3 (untagged)-Human LEM domain containing 3 (LEMD3) transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LEMD3
• Synonyms: MAN1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001167614, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGGCGGCAGCAGCTTCGGCGCCTCAGCAGCTCTCGGATGAGGAGCTTTTCTCTCAGCTCCGCCGTT
  ACGGCCTGTCTCCCGGACCAGTGACGGAGAGCACCCGCCCGGTCTACCTCAAGAAGCTGAAGAAGCTTCG
  AGAGGAAGAGCAGCAACAGCACCGGTCAGGGGGCCGCGGCAACAAGACGCGGAACAGTAATAACAATAAC
  ACGGCAGCCGCCACGGTCGCAGCCGCGGGACCAGCGGCGGCGGCGGCCGCGGGGATGGGGGTCCGGCCGG
  TCTCGGGCGACCTCTCCTACTTACGGACTCCTGGGGGCCTGTGCCGAATCTCGGCCTCTGGCCCAGAGAG
  CCTCCTGGGAGGGCCCGGGGGCGCCTCCGCCGCCCCCGCGGCTGGCAGCAAAGTGCTGCTGGGCTTCAGC
  TCGGACGAGTCGGACGTGGAGGCCAGTCCCCGGGACCAGGCCGGCGGCGGCGGGAGGAAAGACCGGGCTT
  CGCTCCAGTACCGCGGGCTCAAAGCGCCGCCGGCGCCCCTGGCCGCCAGCGAGGTGACTAACAGCAACTC
  TGCAGAGCGAAGGAAGCCCCACTCGTGGTGGGGGGCCAGGAGGCCGGCGGGCCCCGAGCTGCAGACCCCG
  CCGGGGAAAGATGGAGCAGTGGAGGACGAGGAAGGGGAGGGAGAGGACGGTGAGGAGAGGGACCCGGAGA
  CCGAGGAGCCGCTCTGGGCGAGCCGGACCGTGAATGGCAGCCGGCTTGTCCCCTACAGCTGCCGGGAAAA
  CTATTCGGACTCAGAGGAAGAGGACGACGACGACGTGGCCTCCAGCAGACAGGTATTAAAGGACGACTCC
  CTTTCCCGGCATCGGCCCAGACGAACCCATAGTAAGCCTCTCCCCCCGCTGACTGCTAAATCGGCCGGCG
  GCAGGCTGGAGACTTCAGTTCAGGGAGGGGGAGGACTCGCGATGAATGACAGGGCGGCGGCTGCCGGGAG
  TCTAGACAGGAGCCGAAACCTCGAAGAGGCGGCGGCCGCGGAGCAGGGAGGAGGGTGTGATCAAGTGGAC
  TCCAGCCCCGTTCCTAGATACCGTGTTAACGCTAAGAAACTGACCCCTCTCCTGCCCCCGCCACTTACTG
  ACATGGACTCAACCTTGGATTCGTCAACAGGCTCCCTTCTGAAAACCAATAATCATATTGGCGGTGGGGC
  CTTCAGTGTGGACTCCCCCAGGATTTATTCTAACAGTCTCCCTCCCAGTGCGGCGGTGGCCGCCTCTAGT
  TCACTCAGGATCAATCACGCCAATCATACGGGCTCCAATCATACCTACCTGAAAAACACATACAACAAAC
  CGAAGCTTTCCGAACCCGAAGAGGAACTTCTCCAGCAATTTAAACGGGAGGAGGTGTCCCCAACAGGGAG
  TTTCAGTGCCCACTACTTGTCGATGTTTCTCTTAACTGCTGCCTGCTTATTTTTCCTAATACTGGGACTG
  ACTTACCTAGGAATGAGAGGGACAGGAGTATCTGAGGATGGAGAACTCAGCAAAAACCCCTTTGGTGAAA
  CATTTGGAAAAATACAAGAAAGTGAAAAAACTCTTATGATGAACACATTATATAAGCTTCATGATCGATT
  GGCACAGCTTGCAGGAGATCATGAATGTGGCAGTTCTAGTCAAAGAACGCTTTCTGTTCAAGAGGCAGCT
  GCGTATTTAAAAGATTTAGGTCCTGAATATGAAGGTATATTTAACACTTCATTGCAGTGGATCTTAGAAA
  ATGGAAAAGATGTTGGAATAAGGTGTGTTGGTTTTGGCCCTGAGGAAGAATTGACAAATATAACTGATGT
  GCAGTTTTTACAGTCCACAAGACCACTGATGTCTTTTTGGTGTCGTTTTCGACGTGCTTTTGTTACTGTA
  ACTCACAGATTATTGTTGTTATGCTTAGGTGTAGTGATGGTTTGTGTCGTTCTGCGTTACATGAAATATC
  GATGGACAAAAGAAGAGGAGGAAACAAGGCAGATGTATGATATGGTGGTAAAGATTATAGATGTTTTACG
  AAGTCATAATGAAGCCTGCCAGGAAAACAAAGATTTACAACCTTACATGCCTATTCCACATGTACGCGAT
  TCCTTAATACAGCCTCATGACAGGAAAAAAATGAAGAAAGTCTGGGATAGAGCTGTTGACTTCCTTGCTG
  CTAATGAGTCTAGAGTTCGCACGGAAACACGAAGAATAGGTGGTGCAGATTTTCTGGTTTGGCGGTGGAT
  CCAGCCTTCTGCATCCTGTGACAAAATATTAGTTATACCTTCTAAAGTATGGCAAGGTCAAGCATTTCAT
  TTAGATAGAAGAAATTCACCACCAAATAGTTTGACACCGTGTCTAAAGATTCGGAATATGTTTGATCCCG
  TTATGGAAATAGGGGATCAGTGGCATTTGGCAATTCAAGAAGCAATTTTAGAAAAATGCAGTGATAATGA
  TGGCATTGTTCACATTGCAGTAGACAAAAATTCACGTGAGGGTTGTGTATATGTTAAATGTCTGTCTCCA
  GAATATGCTGGAAAGGCTTTTAAAGCATTGCATGGCTCTTGGTTTGATGGGAAATTGGTTACAGTAAAAT
  ATTTACGACTAGATAGATACCACCATCGCTTTCCCCAGGCTCTCACTTCCAACACTCCATTGAAGCCATC
  AAATAAACATATGAACTCCATGTCTCATCTTCGTCTTCGGACTGGCCTAACCAATTCTCAAGGAAGTTCC
  TGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001167614
• ORF Size: 2733 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_001167614.1, NP_001161086.1
• RefSeq Size: 4795
• RefSeq ORF: 2733
• Locus ID: 23592
• Protein Families: Transmembrane
• Gene Summary: This locus encodes a LEM domain-containing protein. The encoded protein functions to antagonize transforming growth factor-beta signaling at the inner nuclear membrane. Two transcript variants encoding different isoforms have been found for this gene. Mutations in this gene have been associated with osteopoikilosis, Buschke-Ollendorff syndrome and melorheostosis. [provided by RefSeq, Nov 2009]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.