PHF8 (NM_001184897) Human Untagged Clone

CAT#: SC329229

PHF8 (untagged)-Human PHD finger protein 8 (PHF8) transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PHF8
• Synonyms: JHDM1F; KDM7B; MRXSSD; ZNF422
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001184897, the custom clone sequence may differ by one or more nucleotides
  ATGGCCTCGGTGCCGGTGTATTGCCTCTGCCGGCTGCCTTACGATGTGACCCGCTTCATG
  ATCGAGTGTGACATGTGCCAGGACTGGTTTCATGGCAGTTGTGTTGGTGTTGAAGAGGAG
  AAGGCTGCTGACATTGACCTCTACCACTGCCCCAACTGTGAAGTCTTGCATGGGCCCTCC
  ATTATGAAAAAACGCCGTGGATCTTCAAAGGGGCATGATACACACAAGGGGAAACCAGTG
  AAGACCGGGAGCCCTACGTTCGTCAGAGAGCTCCGGAGTAGGACTTTTGACAGCTCAGAT
  GAAGTGATTCTGAAGCCCACTGGAAATCAACTGACCGTGGAATTCCTGGAAGAAAATAGC
  TTCAGTGTGCCCATCCTGGTCCTGAAGAAGGATGGGTTGGGCATGACGCTGCCCTCGCCA
  TCATTCACTGTGAGGGATGTTGAACACTATGTTGGTTCTGACAAAGAGATTGATGTGATT
  GATGTGACCCGCCAGGCTGACTGCAAGATGAAGCTTGGTGATTTTGTGAAATACTATTAC
  AGCGGGAAGAGGGAGAAAGTCCTCAATGTCATTAGTTTGGAATTCTCTGATACCAGACTT
  TCTAACCTTGTGGAGACACCGAAGATTGTTCGAAAGCTGTCATGGGTCGAAAACTTGTGG
  CCAGAGGAATGTGTCTTTGAGAGACCCAATGTACAGAAGTACTGCCTCATGAGTGTGCGA
  GATAGCTATACAGACTTTCACATTGACTTTGGTGGCACCTCTGTCTGGTACCATGTACTC
  AAGGGTGAAAAGATCTTCTACCTGATCCGCCCAACAAATGCCAATCTGACTCTCTTTGAG
  TGCTGGAGCAGTTCCTCTAATCAGAATGAGATGTTCTTTGGGGACCAGGTGGACAAGTGC
  TACAAGTGTTCCGTGAAGCAAGGACAGACACTTTTCATTCCCACAGGGTGGATCCATGCT
  GTGCTGACGCCTGTGGACTGCCTTGCCTTTGGAGGGAACTTCTTACACAGCCTTAACATC
  GAGATGCAGCTCAAAGCCTATGAGATTGAGAAGCGGCTGAGCACAGCAGACCTCTTCAGA
  TTCCCCAACTTTGAGACCATCTGTTGGTATGTGGGAAAGCACATCCTGGACATCTTTCGC
  GGTTTGCGAGAGAACAGGAGACACCCTGCCTCCTACCTGGTCCATGGTGGCAAAGCCTTG
  AACTTGGCCTTTAGAGCCTGGACAAGGAAAGAAGCTCTGCCAGACCATGAGGATGAGATC
  CCGGAGACAGTGCGAACCGTACAGCTCATTAAAGATCTGGCCAGGGAGATCCGCCTGGTG
  GAATTCAACATCACTGGTGCCTGCTTGAATGACTCAGATGACGACTCACCAGACTTGGAC
  CTTGATGGAAATGAGAGCCCATTGGCCCTATTGATGTCTAACGGCAGTACGAAAAGGGTG
  AAGAGTTTATCCAAATCTCGGCGAACCAAGATAGCAAAGAAGGTAGACAAGGCTAGGCTG
  ATGGCAGAACAGGTGATGGAAGACGAATTTGACTTGGATTCAGATGATGAGCTGCAGATT
  GACGAGAGATTGGGAAAGGAGAAGGCGACCCTGATAATAAGACCAAAATTTCCCCGGAAA
  TTGCCCCGTGCGAAGCCTTGCTCTGACCCCAACCGAGTTCGTGAACCAGGAGAAGTTGAG
  TTTGACATTGAGGAGGACTATACAACAGATGAGGACATGGTGGAAGGGGTTGAAGGCAAG
  CTTGGGAATGGTAGTGGCGCTGGTGGCATTCTTGATCTGCTCAAGGCCAGCAGGCAGGTG
  GGGGGACCTGACTATGCTGCCCTCACCGAGGCCCCAGCTTCTCCCAGCACTCAGGAGGCC
  ATCCAGGGCATGCTGTGCATGGCCAACCTGCAGTCCTCATCGTCCTCACCGGCTACCTCT
  AGCCTGCAGGCCTGGTGGACTGGGGGACAGGATCGAAGCAGTGGGAGCTCCAGCAGTGGG
  CTGGGCACAGTGTCTAACAGTCCTGCTTCCCAGCGCACCCCAGGGAAGCGGCCCATCAAG
  CGGCCAGCATACTGGAGAACCGAGAGCGAGGAGGAGGAGGAGAACGCCAGTCTGGATGAA
  CAGGACAGCTTGGGAGCGTGCTTCAAGGATGCAGAGTATATCTATCCTTCACTGGAGTCT
  GATGATGATGACCCTGCTTTGAAATCTCGACCCAAGAAAAAGAAGAATTCAGATGATGCT
  CCATGGAGTCCTAAAGCCCGCGTGACCCCAACTCTGCCGAAGCAGGACCGTCCTGTGCGT
  GAGGGGACCCGGGTAGCCTCTATTGAGACAGGTTTGGCTGCAGCAGCTGCAAAGCTGGCC
  CAGCAGGAGCTACAGAAGGCCCAAAAGAAGAAATATATCAAGAAGAAGCCTTTGCTGAAG
  GAGGTAGAACAGCCTCGCCCTCAAGACTCCAATCTCAGTCTGACAGTACCAGCCCCCACT
  GTGGCTGCCACACCACAACTTGTCACCTCCTCCTCACCCCTGCCTCCTCCTGAGCCTAAA
  CAAGAGGCCCTGTCAGGAAGTCTCGCTGACCATGAGTACACCGCTCGTCCCAATGCCTTT
  GGCATGGCCCAGGCAAACCGCAGCACCACACCTATGGCCCCCGGTGTCTTCTTGACCCAG
  CGGCGCCCTTCAGTTGGCTCCCAGAGCAATCAGGCAGGACAAGGAAAGCGTCCCAAAAAG
  GGCCTGGCCACAGCAAAGCAGAGACTCGGCCGTATCCTGAAAATCCACAGAAATGGCAAA
  CTACTTCTACGACAGGTCATCGTTCAAGCAGAATGCAGACAGGCCATTCACGAGCCCAAG
  TTGAAGAGAAGAGACGCCCATCCGTGA
  
• Restriction Sites: Please inquire
• ACCN: NM_001184897
• ORF Size: 2847 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_001184897.1, NP_001171826.1
• RefSeq Size: 4667
• RefSeq ORF: 2847
• Locus ID: 23133
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: The protein encoded by this gene is a histone lysine demethylase that preferentially acts on histones in the monomethyl or dimethyl states. The encoded protein requires Fe(2+) ion, 2-oxoglutarate, and oxygen for its catalytic activity. The protein has an N-terminal PHD finger and a central Jumonji C domain. This gene is thought to function as a transcription activator. Defects in this gene are a cause of syndromic X-linked Siderius type intellectual disability (MRXSSD) and over-expression of this gene is associated with several forms of cancer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2017]
  Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence, the 3' UTR and coding sequence, and lacks an alternate in-frame exon compared to variant 1. The resulting isoform (3) has a shorter N-terminus, longer and distinct C-terminus, and lacks an internal segment compared to isoform 1.