MCF2 (NM_001171876) Human Untagged Clone

CAT#: SC329248

MCF2 (untagged)-Human MCF.2 cell line derived transforming sequence (MCF2) transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MCF2
• Synonyms: ARHGEF21; DBL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene SC329248 ORF sequence for NM_001171876, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCAAGACATCGCCTTCTTGTCTGGTGGCCGGGGAAAGGACAATGCTTGGATCATTACGTTTCCAGAAA
  ACTGTAATTTTAGATGTATACCAGAGGAAGTAATAGCAAAAGTACTTACTTACCTGACATCTATTGCAAG
  GCAAAATGGATCAGACTCCCGGTTTACCATTATTCTGGATCGAAGATTGGATACATGGTCTTCTCTCAAA
  ATCTCTCTCCAAAAAATCTCGGCTTCCTTCCCTGGGAACTTGCACTTGGTTTTGGTTTTACGTCCTACCA
  GCTTTCTTCAACGAACGTTCACAGACATTGGATTTTGGTTTAGTCAGGAGGATTTTATGCTTAAATTACC
  AGTTGTTATGCTGAGCTCAGTTAGTGATTTGCTGACATACATTGATGACAAGCAATTAACCCCTGAGTTA
  GGCGGCACCTTGCAGTACTGCCACAGTGAATGGATCATCTTCAGAAATGCTATAGAAAATTTTGCCCTCA
  CAGTGAAAGAAATGGCTCAGATGTTACAGTCCTTTGGAACTGAACTGGCTGAGACAGAACTACCAGATGA
  TATTCCCTCAATAGAAGAAATTCTGGCAATTCGTGCTGAAAGGTATCATCTGTTGAAGAATGATATTACA
  GCTGTAACCAAAGAAGGAAAAATTCTGCTAACAAATCTGGAAGTGCCTGACACTGAAGGAGCTGTCAGTT
  CAAGACTAGAATGTCATCGGCAAATAAGTGGTGACTGGCAAACTATTAATAAGTTGCTGACTCAAGTACA
  TGATATGGAAACAGCTTTTGATGGATTTTGGGAAAAACATCAATTAAAAATGGAGCAGTATCTGCAACTA
  TGGAAGTTTGAGCAGGATTTTCAACAGCTTGTGACTGAAGTTGAATTTCTATTAAACCAACAAGCAGAAC
  TGGCTGATGTAACAGGGACTATAGCTCAAGTAAAACAAAAAATAAAAAAATTGGAAAACTTAGATGAAAA
  TTCTCAGGAGCTATTATCAAAGGCCCAGTTTGTGATATTACATGGACACAAGCTTGCAGCAAATCACCAT
  TATGCACTTGATTTAATCTGCCAGAGGTGCAATGAGCTACGTTACCTTTCTGATATTTTGGTTAATGAGA
  TAAAAGCAAAACGGATACAACTCAGCAGGACCTTCAAAATGCATAAACTCCTACAGCAGGCTCGTCAATG
  CTGTGATGAAGGGGAATGTCTTCTAGCTAATCAGGAAATAGATAAGTTTCAGTCTAAAGAAGATGCTCAG
  AAAGCTCTCCAAGACATTGAAAATTTTCTTGAAATGGCTCTACCCTTTATAAATTATGAACCTGAAACAC
  TGCAGTATGAATTTGATGTAATATTATCTCCTGAGCTTAAGGTTCAAATGAAGACTATACAACTCAAGCT
  TGAAAACATTCGAAGTATATTTGAGAACCAGCAGGCTGGTTTCAGGAACCTGGCAGATAAGCATGTGAGG
  CCAATCCAATTTGTGGTACCCACACCTGAAAATTTGGTCACATCTGGGACACCATTTTTTTCATCTAAAC
  AAGTGGGAGTTGGATACTCTTTCTTTCAAGCATGTAAACTTTTTTCAAAAGGGAAGAAGACTTGGAGACA
  AAATCAGAGCAACTTAAAAATTGAAGTGGTGCCTGATTGTCAGGAGAAGAGAAGTTCTGGTCCATCCTCC
  AGTTTGGACAATGGCAATAGCTTGGATGTTTTAAAGAACCACGTACTAAATGAACTGATACAGACTGAGA
  GAGTTTATGTTCGAGAACTGTATACTGTTTTGTTGGGTTATAGAGCGGAGATGGATAATCCAGAGATGTT
  TGATCTTATGCCACCTCTCCTGAGAAATAAAAAGGACATTCTCTTTGGAAACATGGCAGAAATATATGAA
  TTCCATAACGACATTTTCTTGAGCAGCCTGGAAAATTGTGCTCATGCTCCAGAAAGAGTGGGACCTTGTT
  TCCTGGAAAGGAAGGATGATTTTCAGATGTATGCAAAATATTGTCAGAATAAGCCCAGATCAGAAACAAT
  TTGGAGGAAGTATTCAGAATGCGCATTTTTCCAGGAATGTCAAAGAAAGTTAAAACACAGACTTAGACTG
  GATTCCTATTTACTCAAACCAGTGCAACGAATCACTAAATATCAGTTATTGTTGAAGGAGCTATTAAAAT
  ATAGCAAAGACTGTGAAGGATCTGCTCTGTTGAAGAAGGCACTCGATGCAATGCTGGATTTACTGAAGTC
  AGTTAATGATTCTATGCATCAGATTGCAATAAATGGCTATATTGGAAACTTAAATGAACTGGGCAAGATG
  ATAATGCAAGGTGGATTCAGCGTTTGGATAGGGCACAAGAAAGGTGCTACAAAAATGAAGGATTTGGCTA
  GATTCAAACCAATGCAGCGACACCTTTTCTTGTATGAAAAAGCCATTGTTTTTTGCAAAAGGCGTGTTGA
  AAGTGGAGAAGGCTCTGACAGATACCCGTCATACAGTTTTAAACACTGTTGGAAAATGGATGAAGTTGGA
  ATCACTGAATATGTAAAAGGTGATAACCGCAAGTTTGAAATCTGGTATGGTGAAAAGGAAGAAGTTTATA
  TTGTCCAGGCTTCTAATGTAGATGTGAAGATGACGTGGCTAAAAGAAATAAGAAATATTTTGTTGAAGCA
  GCAGGAACTTTTGACAGTTAAAAAAAGAAAGCAACAGGATCAATTAACAGAACGGGATAAGTTTCAGATT
  TCTCTTCAGCAGAATGATGAAAAGCAACAGGGAGCTTTTATAAGTACTGAGGAAACTGAATTGGAACACA
  CCAGCACTGTGGTGGAGGTCTGTGAGGCAATTGCGTCAGTTCAGGCAGAAGCAAATACAGTTTGGACTGA
  GGCATCACAATCTGCAGAAATCTCTGAAGAACCTGCGGAATGGTCAAGCAACTATTTCTACCCTACTTAT
  GATGAAAATGAAGAAGAAAATAGGCCCCTCATGAGACCTGTGTCGGAGATGGCTCTCCTATATTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001171876
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001171876.1, NP_001165347.1
• RefSeq Size: 3867 bp
• RefSeq ORF: 3006 bp
• Locus ID: 4168
• Cytogenetics: Xq27.1
• Protein Families: Druggable Genome
• Gene Summary: 'The oncogenic protein encoded by this gene is a guanine nucleotide exchange factor (GEF) that exerts control over some members of the Rho family of small GTPases. Several transcript variants encoding different isoforms have been found for this gene. These isoforms exhibit different expression patterns and varying levels of GEF activity.[provided by RefSeq, Jan 2010]'
  Transcript Variant: This variant (3) represents the longest transcript and encodes the longest isoform (c).