AREB6 (ZEB1) (NM_001174094) Human Untagged Clone

CAT#: SC329289

ZEB1 (untagged)-Human zinc finger E-box binding homeobox 1 (ZEB1) transcript variant 7

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ZEB1
• Synonyms: AREB6; BZP; DELTAEF1; FECD6; NIL2A; PPCD3; TCF8; ZFHEP; ZFHX1A
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001174094, the custom clone sequence may differ by one or more nucleotides
  ATGAAAGTTACAAATTATAATACTGTGGTAGAAACAAATTCAGATTCAGATGATGAAGAC
  AAACTGCATATTGTGGAAGAAGAAAGTGTTACAGATGCAGCTGACTGTGAAGGTGTACCA
  GAGGATGACCTGCCAACAGACCAGACAGTGTTACCAGGGAGGAGCAGTGAAAGAGAAGGG
  AATGCTAAGAACTGCTGGGAGGATGACAGAAAGGAAGGGCAAGAAATCCTGGGGCCTGAA
  GCTCAGGCAGATGAAGCAGGATGTACAGTAAAAGATGATGAATGCGAGTCAGATGCAGAA
  AATGAGCAAAACCATGATCCTAATGTTGAAGAGTTTCTACAACAACAAGACACTGCTGTC
  ATTTTTCCTGAGGCACCTGAAGAGGACCAGAGGCAGGGCACACCAGAAGCCAGTGGTCAT
  GATGAAAATGGAACACCAGATGCATTTTCACAATTACTCACCTGTCCATATTGTGATAGA
  GGCTATAAACGCTTTACCTCTCTGAAAGAACACATTAAATATCGTCATGAAAAGAATGAA
  GATAACTTTAGTTGCTCCCTGTGCAGTTACACCTTTGCATACAGAACCCAACTTGAACGT
  CACATGACATCACATAAATCAGGAAGAGATCAAAGACATGTGACGCAGTCTGGGTGTAAT
  CGTAAATTCAAATGCACTGAGTGTGGAAAAGCTTTCAAATACAAACATCACCTAAAAGAG
  CACTTAAGAATTCACAGTGGAGAGAAGCCATATGAATGCCCAAACTGCAAGAAACGCTTT
  TCCCATTCTGGCTCCTATAGCTCACACATAAGCAGTAAGAAATGTATCAGCTTGATACCT
  GTGAATGGGCGACCAAGAACAGGACTCAAGACATCTCAGTGTTCTTCACCGTCTCTTTCA
  GCATCACCAGGCAGTCCCACACGACCACAGATACGGCAAAAGATAGAGAATAAACCCCTT
  CAAGAACAACTTTCTGTTAACCAAATTAAAACTGAACCTGTGGATTATGAATTCAAACCC
  ATAGTGGTTGCTTCAGGAATCAACTGTTCAACCCCTTTACAAAATGGGGTTTTCACTGGT
  GGTGGCCCATTACAGGCAACCAGTTCTCCTCAGGGCATGGTGCAAGCTGTTGTTCTGCCA
  ACAGTTGGTTTGGTGTCTCCCATAAGTATCAATTTAAGTGATATTCAGAATGTACTTAAA
  GTGGCGGTAGATGGTAATGTAATAAGGCAAGTGTTGGAGAATAATCAAGCCAATCTTGCA
  TCCAAAGAACAAGAAACAATCAATGCTTCACCCATACAACAAGGTGGCCATTCTGTTATT
  TCAGCCATCAGTCTTCCTTTGGTTGATCAAGATGGAACAACCAAAATTATCATCAACTAC
  AGTCTTGAGCAGCCTAGCCAACTTCAAGTTGTTCCTCAAAATTTAAAAAAAGAAAATCCA
  GTCGCTACAAACAGTTGTAAAAGTGAAAAGTTACCAGAAGATCTTACTGTTAAGTCTGAG
  AAGGACAAAAGCTTTGAAGGGGGGGTGAATGATAGCACTTGTCTTCTGTGTGATGATTGT
  CCAGGAGATATTAATGCACTTCCAGAATTAAAGCACTATGACCTAAAGCAGCCTACTCAG
  CCTCCTCCACTCCCTGCAGCAGAAGCTGAGAAGCCTGAGTCCTCTGTTTCATCAGCTACT
  GGAGATGGCAATTTGTCTCCTAGTCAGCCACCTTTAAAGAACCTCTTGTCTCTCCTAAAA
  GCATATTATGCTTTGAATGCACAACCAAGTGCAGAAGAGCTCTCAAAAATTGCTGATTCA
  GTAAACCTACCACTGGATGTAGTAAAAAAGTGGTTTGAAAAGATGCAAGCTGGACAGATT
  TCAGTGCAGTCTTCTGAACCATCTTCTCCTGAACCAGGCAAAGTAAATATCCCTGCCAAG
  AACAATGATCAGCCTCAATCTGCAAATGCAAATGAACCCCAGGACAGCACAGTAAATCTA
  CAAAGTCCTTTGAAGATGACTAACTCCCCAGTTTTACCAGTGGGATCAACCACCAATGGT
  TCCAGAAGTAGTACACCATCCCCATCACCTCTAAACCTTTCCTCATCCAGAAATACACAG
  GGTTACTTGTACACAGCTGAGGGTGCACAAGAAGAGCCACAAGTAGAACCTCTTGATCTT
  TCACTACCAAAGCAACAGGGAGAATTATTAGAAAGGTCAACTATCACTAGTGTTTACCAG
  AACAGTGTTTATTCTGTCCAGGAAGAACCCTTGAACTTGTCTTGCGCAAAAAAGGAGCCA
  CAAAAGGACAGTTGTGTTACAGACTCAGAACCAGTTGTAAATGTAATCCCACCAAGTGCC
  AACCCCATAAATATCGCTATACCTACAGTCACTGCCCAGTTACCCACAATCGTGGCCATT
  GCTGACCAGAACAGTGTTCCATGCTTAAGAGCGCTAGCTGCCAATAAGCAAACGATTCTG
  ATTCCCCAGGTGGCATACACCTACTCAACTACGGTCAGCCCTGCAGTCCAAGAACCACCC
  TTGAAAGTGATCCAGCCAAATGGAAATCAGGATGAAAGACAAGATACTAGCTCAGAAGGA
  GTATCAAATGTAGAGGATCAGAATGACTCTGATTCTACACCGCCCAAAAAGAAAATGCGG
  AAGACAGAAAATGGAATGTATGCTTGTGATTTGTGTGACAAGATATTCCAAAAGAGTAGT
  TCATTATTGAGACATAAATATGAACACACAGGTAAAAGACCTCATGAGTGTGGAATCTGT
  AAAAAGGCATTTAAACACAAACATCATTTGATTGAACACATGCGATTACATTCTGGAGAA
  AAGCCCTATCAATGTGACAAATGTGGAAAGCGCTTCTCACACTCTGGGTCTTATTCTCAA
  CACATGAATCATCGCTACTCCTACTGTAAGAGAGAAGCGGAAGAACGTGACAGCACAGAG
  CAGGAAGAGGCAGGGCCTGAAATCCTCTCGAATGAGCACGTGGGTGCCAGGGCGTCTCCC
  TCACAGGGCGACTCGGACGAGAGAGAGAGTTTGACAAGGGAAGAGGATGAAGACAGTGAA
  AAAGAGGAAGAGGAGGAGGATAAAGAGATGGAAGAATTGCAGGAAGAAAAAGAATGTGAA
  AAACCACAAGGGGATGAGGAAGAGGAGGAGGAGGAGGAAGAAGTGGAAGAAGAAGAGGTA
  GAAGAGGCAGAGAATGAGGGAGAAGAAGCAAAAACTGAAGGTCTGATGAAGGATGACAGG
  GCTGAAAGTCAAGCAAGCAGCTTAGGACAAAAAGTAGGCGAGAGTAGTGAGCAAGTGTCT
  GAAGAAAAGACAAATGAAGCCTAA
  
• Restriction Sites: Please inquire
• ACCN: NM_001174094
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001174094.1, NP_001167565.1
• RefSeq Size: 6275 bp
• RefSeq ORF: 3324 bp
• Locus ID: 6935
• Cytogenetics: 10p11.22
• Protein Families: Transcription Factors
• Gene Summary: 'This gene encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in this gene have been associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Mar 2010]'
  Transcript Variant: This variant (7) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. This results in a shorter protein (isoform d), compared to isoform a.