AREB6 (ZEB1) (NM_001174096) Human Untagged Clone

CAT#: SC329294

ZEB1 (untagged)-Human zinc finger E-box binding homeobox 1 (ZEB1) transcript variant 9

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ZEB1
• Synonyms: AREB6; BZP; DELTAEF1; FECD6; NIL2A; PPCD3; TCF8; ZFHEP; ZFHX1A
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001174096, the custom clone sequence may differ by one or more nucleotides
  ATGGCGGATGGCCCCAGGTGTAAGCGCAGAAAGCAGGCGAACCCGCGGCGCAATAACGTT
  ACAAATTATAATACTGTGGTAGAAACAAATTCAGATTCAGATGATGAAGACAAACTGCAT
  ATTGTGGAAGAAGAAAGTGTTACAGATGCAGCTGACTGTGAAGGTGTACCAGAGGATGAC
  CTGCCAACAGACCAGACAGTGTTACCAGGGAGGAGCAGTGAAAGAGAAGGGAATGCTAAG
  AACTGCTGGGAGGATGACACAGGAAAGGAAGGGCAAGAAATCCTGGGGCCTGAAGCTCAG
  GCAGATGAAGCAGGATGTACAGTAAAAGATGATGAATGCGAGTCAGATGCAGAAAATGAG
  CAAAACCATGATCCTAATGTTGAAGAGTTTCTACAACAACAAGACACTGCTGTCATTTTT
  CCTGAGGCACCTGAAGAGGACCAGAGGCAGGGCACACCAGAAGCCAGTGGTCATGATGAA
  AATGGAACACCAGATGCATTTTCACAATTACTCACCTGTCCATATTGTGATAGAGGCTAT
  AAACGCTTTACCTCTCTGAAAGAACACATTAAATATCGTCATGAAAAGAATGAAGATAAC
  TTTAGTTGCTCCCTGTGCAGTTACACCTTTGCATACAGAACCCAACTTGAACGTCACATG
  ACATCACATAAATCAGGAAGAGATCAAAGACATGTGACGCAGTCTGGGTGTAATCGTAAA
  TTCAAATGCACTGAGTGTGGAAAAGCTTTCAAATACAAACATCACCTAAAAGAGCACTTA
  AGAATTCACAGTGGAGAGAAGCCATATGAATGCCCAAACTGCAAGAAACGCTTTTCCCAT
  TCTGGCTCCTATAGCTCACACATAAGCAGTAAGAAATGTATCAGCTTGATACCTGTGAAT
  GGGCGACCAAGAACAGGACTCAAGACATCTCAGTGTTCTTCACCGTCTCTTTCAGCATCA
  CCAGGCAGTCCCACACGACCACAGATACGGCAAAAGATAGAGAATAAACCCCTTCAAGAA
  CAACTTTCTGTTAACCAAATTAAAACTGAACCTGTGGATTATGAATTCAAACCCATAGTG
  GTTGCTTCAGGAATCAACTGTTCAACCCCTTTACAAAATGGGGTTTTCACTGGTGGTGGC
  CCATTACAGGCAACCAGTTCTCCTCAGGGCATGGTGCAAGCTGTTGTTCTGCCAACAGTT
  GGTTTGGTGTCTCCCATAAGTATCAATTTAAGTGATATTCAGAATGTACTTAAAGTGGCG
  GTAGATGGTAATGTAATAAGGCAAGTGTTGGAGAATAATCAAGCCAATCTTGCATCCAAA
  GAACAAGAAACAATCAATGCTTCACCCATACAACAAGGTGGCCATTCTGTTATTTCAGCC
  ATCAGTCTTCCTTTGGTTGATCAAGATGGAACAACCAAAATTATCATCAACTACAGTCTT
  GAGCAGCCTAGCCAACTTCAAGTTGTTCCTCAAAATTTAAAAAAAGAAAATCCAGTCGCT
  ACAAACAGTTGTAAAAGTGAAAAGTTACCAGAAGATCTTACTGTTAAGTCTGAGAAGGAC
  AAAAGCTTTGAAGGGGGGGTGAATGATAGCACTTGTCTTCTGTGTGATGATTGTCCAGGA
  GATATTAATGCACTTCCAGAATTAAAGCACTATGACCTAAAGCAGCCTACTCAGCCTCCT
  CCACTCCCTGCAGCAGAAGCTGAGAAGCCTGAGTCCTCTGTTTCATCAGCTACTGGAGAT
  GGCAATTTGTCTCCTAGTCAGCCACCTTTAAAGAACCTCTTGTCTCTCCTAAAAGCATAT
  TATGCTTTGAATGCACAACCAAGTGCAGAAGAGCTCTCAAAAATTGCTGATTCAGTAAAC
  CTACCACTGGATGTAGTAAAAAAGTGGTTTGAAAAGATGCAAGCTGGACAGATTTCAGTG
  CAGTCTTCTGAACCATCTTCTCCTGAACCAGGCAAAGTAAATATCCCTGCCAAGAACAAT
  GATCAGCCTCAATCTGCAAATGCAAATGAACCCCAGGACAGCACAGTAAATCTACAAAGT
  CCTTTGAAGATGACTAACTCCCCAGTTTTACCAGTGGGATCAACCACCAATGGTTCCAGA
  AGTAGTACACCATCCCCATCACCTCTAAACCTTTCCTCATCCAGAAATACACAGGGTTAC
  TTGTACACAGCTGAGGGTGCACAAGAAGAGCCACAAGTAGAACCTCTTGATCTTTCACTA
  CCAAAGCAACAGGGAGAATTATTAGAAAGGTCAACTATCACTAGTGTTTACCAGAACAGT
  GTTTATTCTGTCCAGGAAGAACCCTTGAACTTGTCTTGCGCAAAAAAGGAGCCACAAAAG
  GACAGTTGTGTTACAGACTCAGAACCAGTTGTAAATGTAATCCCACCAAGTGCCAACCCC
  ATAAATATCGCTATACCTACAGTCACTGCCCAGTTACCCACAATCGTGGCCATTGCTGAC
  CAGAACAGTGTTCCATGCTTAAGAGCGCTAGCTGCCAATAAGCAAACGATTCTGATTCCC
  CAGGTGGCATACACCTACTCAACTACGGTCAGCCCTGCAGTCCAAGAACCACCCTTGAAA
  GTGATCCAGCCAAATGGAAATCAGGATGAAAGACAAGATACTAGCTCAGAAGGAGTATCA
  AATGTAGAGGATCAGAATGACTCTGATTCTACACCGCCCAAAAAGAAAATGCGGAAGACA
  GAAAATGGAATGTATGCTTGTGATTTGTGTGACAAGATATTCCAAAAGAGTAGTTCATTA
  TTGAGACATAAATATGAACACACAGGTAAAAGACCTCATGAGTGTGGAATCTGTAAAAAG
  GCATTTAAACACAAACATCATTTGATTGAACACATGCGATTACATTCTGGAGAAAAGCCC
  TATCAATGTGACAAATGTGGAAAGCGCTTCTCACACTCTGGGTCTTATTCTCAACACATG
  AATCATCGCTACTCCTACTGTAAGAGAGAAGCGGAAGAACGTGACAGCACAGAGCAGGAA
  GAGGCAGGGCCTGAAATCCTCTCGAATGAGCACGTGGGTGCCAGGGCGTCTCCCTCACAG
  GGCGACTCGGACGAGAGAGAGAGTTTGACAAGGGAAGAGGATGAAGACAGTGAAAAAGAG
  GAAGAGGAGGAGGATAAAGAGATGGAAGAATTGCAGGAAGAAAAAGAATGTGAAAAACCA
  CAAGGGGATGAGGAAGAGGAGGAGGAGGAGGAAGAAGTGGAAGAAGAAGAGGTAGAAGAG
  GCAGAGAATGAGGGAGAAGAAGCAAAAACTGAAGGTCTGATGAAGGATGACAGGGCTGAA
  AGTCAAGCAAGCAGCTTAGGACAAAAAGTAGGCGAGAGTAGTGAGCAAGTGTCTGAAGAA
  AAGACAAATGAAGCCTAA
  
• Restriction Sites: Please inquire
• ACCN: NM_001174096
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001174096.1, NP_001167567.1
• RefSeq Size: 6001 bp
• RefSeq ORF: 3378 bp
• Locus ID: 6935
• Cytogenetics: 10p11.22
• Protein Families: Transcription Factors
• Gene Summary: 'This gene encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in this gene have been associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Mar 2010]'
  Transcript Variant: This variant (9) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (f) is longer and has a distinct N-terminus, compared to isoform a.