GPR35 (NM_001195382) Human Untagged Clone

CAT#: SC329454

GPR35 (untagged) - Homo sapiens G protein-coupled receptor 35 (GPR35), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GPR35
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001195382, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCTGAGTGGTTCCCGGGCTGTCCCCACTCCACACCGTGGCAGTGAAGAGCTGCTGAAGTACATGCTTC
  ATAGTCCTTGCGTCTCTCTGACCATGAATGGCACCTACAACACCTGTGGCTCCAGCGACCTCACCTGGCC
  CCCAGCGATCAAGCTGGGCTTCTACGCCTACTTGGGCGTCCTGCTGGTGCTAGGCCTGCTGCTCAACAGC
  CTGGCGCTCTGGGTGTTCTGCTGCCGCATGCAGCAGTGGACGGAGACCCGCATCTACATGACCAACCTGG
  CGGTGGCCGACCTCTGCCTGCTGTGCACCTTGCCCTTCGTGCTGCACTCCCTGCGAGACACCTCAGACAC
  GCCGCTGTGCCAGCTCTCCCAGGGCATCTACCTGACCAACAGGTACATGAGCATCAGCCTGGTCACGGCC
  ATCGCCGTGGACCGCTATGTGGCCGTGCGGCACCCGCTGCGTGCCCGCGGGCTGCGGTCCCCCAGGCAGG
  CTGCGGCCGTGTGCGCGGTCCTCTGGGTGCTGGTCATCGGCTCCCTGGTGGCTCGCTGGCTCCTGGGGAT
  TCAGGAGGGCGGCTTCTGCTTCAGGAGCACCCGGCACAATTTCAACTCCATGGCGTTCCCGCTGCTGGGA
  TTCTACCTGCCCCTGGCCGTGGTGGTCTTCTGCTCCCTGAAGGTGGTGACTGCCCTGGCCCAGAGGCCAC
  CCACCGACGTGGGGCAGGCAGAGGCCACCCGCAAGGCTGCCCGCATGGTCTGGGCCAACCTCCTGGTGTT
  CGTGGTCTGCTTCCTGCCCCTGCACGTGGGGCTGACAGTGCGCCTCGCAGTGGGCTGGAACGCCTGTGCC
  CTCCTGGAGACGATCCGTCGCGCCCTGTACATAACCAGCAAGCTCTCAGATGCCAACTGCTGCCTGGACG
  CCATCTGCTACTACTACATGGCCAAGGAGTTCCAGGAGGCGTCTGCACTGGCCGTGGCTCCCAGTGCTAA
  GGCCCACAAAAGCCAGGACTCTCTGTGCGTGACCCTCGCCTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001195382
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001195382.1, NP_001182311.1
• RefSeq Size: 2179 bp
• RefSeq ORF: 1023 bp
• Locus ID: 2859
• Cytogenetics: 2q37.3
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: ''
  Transcript Variant: This variant (3) differs in the 5' UTR and 5' coding region, and uses an alternate start codon, compared to variant 1. The resulting isoform (b, also known as GPR35b) is longer at the N-terminus, compared to isoform a. Both variants 2 and 3 encode the same isoform (b).