GPR35 (NM_001195382) Human Tagged ORF Clone

CAT#: RG232526

GPR35 (GFP-tagged) - Homo sapiens G protein-coupled receptor 35 (GPR35), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: GPR35
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG232526 representing NM_001195382
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGAGTGGTTCCCGGGCTGTCCCCACTCCACACCGTGGCAGTGAAGAGCTGCTGAAGTACATGCTTC
  ATAGTCCTTGCGTCTCTCTGACCATGAATGGCACCTACAACACCTGTGGCTCCAGCGACCTCACCTGGCC
  CCCAGCGATCAAGCTGGGCTTCTACGCCTACTTGGGCGTCCTGCTGGTGCTAGGCCTGCTGCTCAACAGC
  CTGGCGCTCTGGGTGTTCTGCTGCCGCATGCAGCAGTGGACGGAGACCCGCATCTACATGACCAACCTGG
  CGGTGGCCGACCTCTGCCTGCTGTGCACCTTGCCCTTCGTGCTGCACTCCCTGCGAGACACCTCAGACAC
  GCCGCTGTGCCAGCTCTCCCAGGGCATCTACCTGACCAACAGGTACATGAGCATCAGCCTGGTCACGGCC
  ATCGCCGTGGACCGCTATGTGGCCGTGCGGCACCCGCTGCGTGCCCGCGGGCTGCGGTCCCCCAGGCAGG
  CTGCGGCCGTGTGCGCGGTCCTCTGGGTGCTGGTCATCGGCTCCCTGGTGGCTCGCTGGCTCCTGGGGAT
  TCAGGAGGGCGGCTTCTGCTTCAGGAGCACCCGGCACAATTTCAACTCCATGGCGTTCCCGCTGCTGGGA
  TTCTACCTGCCCCTGGCCGTGGTGGTCTTCTGCTCCCTGAAGGTGGTGACTGCCCTGGCCCAGAGGCCAC
  CCACCGACGTGGGGCAGGCAGAGGCCACCCGCAAGGCTGCCCGCATGGTCTGGGCCAACCTCCTGGTGTT
  CGTGGTCTGCTTCCTGCCCCTGCACGTGGGGCTGACAGTGCGCCTCGCAGTGGGCTGGAACGCCTGTGCC
  CTCCTGGAGACGATCCGTCGCGCCCTGTACATAACCAGCAAGCTCTCAGATGCCAACTGCTGCCTGGACG
  CCATCTGCTACTACTACATGGCCAAGGAGTTCCAGGAGGCGTCTGCACTGGCCGTGGCTCCCAGTGCTAA
  GGCCCACAAAAGCCAGGACTCTCTGTGCGTGACCCTCGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG232526 representing NM_001195382
  Red=Cloning site Green=Tags(s)
  
  MLSGSRAVPTPHRGSEELLKYMLHSPCVSLTMNGTYNTCGSSDLTWPPAIKLGFYAYLGVLLVLGLLLNS
  LALWVFCCRMQQWTETRIYMTNLAVADLCLLCTLPFVLHSLRDTSDTPLCQLSQGIYLTNRYMSISLVTA
  IAVDRYVAVRHPLRARGLRSPRQAAAVCAVLWVLVIGSLVARWLLGIQEGGFCFRSTRHNFNSMAFPLLG
  FYLPLAVVVFCSLKVVTALAQRPPTDVGQAEATRKAARMVWANLLVFVVCFLPLHVGLTVRLAVGWNACA
  LLETIRRALYITSKLSDANCCLDAICYYYMAKEFQEASALAVAPSAKAHKSQDSLCVTLA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001195382
• ORF Size: 1020 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001195382.1, NP_001182311.1
• RefSeq Size: 2179 bp
• RefSeq ORF: 1023 bp
• Locus ID: 2859
• Cytogenetics: 2q37.3
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: ''