PDE8A (NM_001243137) Human Untagged Clone

CAT#: SC330089

PDE8A (untagged) - Homo sapiens phosphodiesterase 8A (PDE8A), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PDE8A
• Synonyms: HsT19550
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001243137, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGATTTCATCAAGATCAACTTCAGGTACTTTTAGTGTTTACCAAAGAAGATAACCAATGTAATGGAT
  TCTGCAGGGCATGTGAAAAAGCAGGGTTTAAGTGTACAGTTACCAAGGAGGCTCAGGCTGTCCTTGCCTG
  TTTCCTGGACAAACATCATGACATTATCATCATAGACCACAGAAATCCTCGACAGCTGGATGCAGAGGCA
  CTGTGCAGGTCTATCAGATCATCAAAACTCTCAGAAAACACAGTTATTGTTGGTGTAGTACGCAGGGTGG
  ATAGAGAAGAGTTGTCCGTAATGCCTTTCATTTCTGCTGGATTTACAAGGAGGTATGTAGAAAACCCCAA
  CATCATGGCCTGCTACAATGAACTGCTCCAGCTGGAGTTTGGAGAGGTGCGATCACAACTGAAACTCAGG
  GCTTGTAACTCAGTATTCACTGCATTAGAAAACAGTGAAGATGCAATTGAAATTACAAGCGAAGACCGTT
  TTATACAGTATGCAAATCCTGCATTTGAAACAACAATGGGCTATCAGTCAGGTGAATTAATAGGGAAGGA
  GTTAGGAGAAGTGCCTATAAATGAAAAAAAGGCTGACTTGCTCGATACTATAAATTCATGCATCAGGATA
  GGCAAGGAGTGGCAAGGAATTTACTATGCCAAAAAGAAAAACGGAGATAATATACAACAAAATGTGAAGA
  TAATACCTGTCATTGGACAGGGAGGAAAAATTAGACACTATGTGTCCATTATCAGAGTGTGCAATGGCAA
  CAATAAGGCTGAGAAAATATCCGAATGTGTTCAGTCTGACACTCATACAGATAATCAGACAGGCAAACAT
  AAAGACAGGAGAAAAGGCTCACTAGACGTCAAAGCTGTTGCCTCCCGTGCAACTGAAGTTTCCAGCCAGA
  GACGACACTCTTCCATGGCCCGGATACATTCCATGACAATTGAGGCGCCCATCACCAAGGTAATCAATAT
  TATCAATGCTGCCCAGGAAAGTAGTCCCATGCCTGTGACAGAAGCCCTAGACCGTGTGCTGGAAATTCTA
  AGAACCACTGAGTTATATTCACCACAGTTTGGTGCTAAAGATGATGATCCCCATGCCAATGACCTTGTTG
  GGGGCTTAATGTCTGATGGTTTGCGAAGACTATCAGGGAATGAATATGTTCTTTCAACAAAAAACACTCA
  AATGGTTTCAAGCAATATAATCACTCCCATCTCCCTTGATGATGTCCCACCACGGATAGCTCGGGCCATG
  GAAAATGAGGAATACTGGGACTTTGATATTTTTGAACTGGAGGCTGCCACCCACAATAGGCCTTTGATTT
  ATCTTGGTCTCAAAATGTTTGCTCGCTTTGGAATCTGTGAATTCTTACACTGCTCCGAGTCAACGCTAAG
  ATCATGGTTACAAATTATCGAAGCCAATTATCATTCCTCCAATCCCTACCACAATTCTACACATTCTGCT
  GATGTGCTTCATGCCACTGCCTATTTTCTCTCCAAGGAGAGGATAAAGGAAACTTTAGATCCAATTGATG
  AGGTCGCTGCACTCATCGCAGCCACCATTCATGATGTGGATCACCCTGGGAGAACCAACTCCTTCCTGTG
  TAATGCTGGAAGTGAGCTGGCCATTTTGTACAATGACACTGCTGTGCTGGAGAGCCACCATGCGGCCTTG
  GCCTTCCAGCTGACCACTGGAGATGATAAATGCAATATATTTAAAAACATGGAGAGGAATGATTATCGGA
  CACTGCGCCAGGGGATTATCGACATGGTCTTAGCCACAGAAATGACAAAGCACTTTGAGCATGTCAACAA
  ATTTGTCAACAGCATCAACAAACCCTTGGCAACACTAGAAGAAAATGGGGAAACTGATAAAAACCAGGAA
  GTGATAAACACTATGCTTAGGACTCCAGAGAACCGGACCCTAATCAAACGAATGCTGATTAAATGTGCTG
  ATGTGTCCAATCCCTGCCGACCCCTGCAGTACTGCATCGAGTGGGCTGCACGCATTTCGGAAGAATATTT
  TTCTCAGACTGATGAAGAGAAGCAGCAGGGCTTACCTGTGGTGATGCCAGTGTTTGACAGAAATACCTGC
  AGCATCCCCAAATCCCAAATCTCTTTCATTGATTACTTCATCACAGACATGTTTGATGCTTGGGATGCCT
  TTGTAGACCTGCCTGATTTAATGCAGCATCTTGACAACAACTTTAAATACTGGAAAGGACTGGACGAAAT
  GAAGCTGCGGAACCTCCGACCACCTCCTGAATAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001243137
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001243137.1, NP_001230066.1
• RefSeq Size: 3728 bp
• RefSeq ORF: 2274 bp
• Locus ID: 5151
• Cytogenetics: 15q25.3
• Protein Families: Druggable Genome
• Protein Pathways: Progesterone-mediated oocyte maturation, Purine metabolism
• Gene Summary: 'The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE8 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]'
  Transcript Variant: This variant (3) contains an alternate 5' terminal exon compared to variant 1. This results in translation initiation from an in-frame downstream AUG, and an isoform (3) with a shorter N-terminus compared to isoform 1.