FE65 (APBB1) (NM_001257319) Human Untagged Clone

CAT#: SC330596

APBB1 (untagged) - Homo sapiens amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65) (APBB1), transcript variant 9

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: APBB1
• Synonyms: FE65; MGC:9072; RIR
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001257319, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGCGCCATGTTCTCCCAGGACTTTTTCCTGGCCATTATCCTGCAGGACAGCAGCGCAGATTCCTTCT
  GGAACCCCAACGCCTTCGAGACGGATTCCGACCTGCCGGCTGGATGGATGAGGGTCCAGGACACCTCAGG
  GACCTATTACTGGCACATCCCAACAGGGACCACCCAGTGGGAACCCCCCGGCCGGGCCTCCCCCTCACAG
  GGGAGCAGCCCCCAAGAGGAGTCCCAGCTCACCTGGACAGGTTTTGCTCATGGAGAAGGCTTTGAGGATG
  GAGAATTTTGGAAGGATGAACCCAGTGATGAGGCCCCAATGGAGCTGGGACTGAAGGAACCTGAGGAGGG
  GACGTTGACCTTCCCAGCTCAGAGCCTCAGCCCAGAGCCGTTGCCCCAAGAGGAGGAGAAGCTTCCCCCA
  CGGAATACCAACCCAGGGATCAAGTGTTTCGCCGTGCGCTCCCTAGGCTGGGTAGAGATGACCGAGGAGG
  AGCTGGCCCCTGGACGCAGCAGTGTGGCAGTCAACAATTGCATCCGTCAGCTCTCTTACCACAAAAACAA
  CCTGCATGACCCCATGTCTGGGGGCTGGGGGGAAGGAAAGGATCTGCTACTGCAGCTGGAGGATGAGACA
  CTAAAGCTAGTGGAGCCACAGAGCCAGGCACTGCTGCACGCCCAACCCATCATCAGCATCCGCGTGTGGG
  GCGTCGGGCGGGACAGTGGAAGAGAGAGGGACTTTGCCTACGTAGCTCGTGATAAGCTGACCCAGATGCT
  CAAGTGCCACGTGTTTCGCTGTGAGGCACCTGCCAAGAACATCGCCACCAGCCTGCATGAGATCTGCTCT
  AAGATCATGGCCGAACGGCGTAATGCCCGCTGCTTGGTAAATGGACTCTCCCTGGACCACTCTAAACTTG
  TGGATGTCCCTTTCCAAGTGGAATTCCCAGCGCCTAAGAATGAGTTGGTCCAGAAGTTCCAAGTCTATTA
  CCTGGGGAATGTACCTGTTGCTAAACCTGTTGGGGTAGATGTGATTAATGGGGCCCTCGAGTCAGTCCTG
  TCCTCCAGCAGCCGTGAACAATGGACCCCAAGTCATGTCAGTGTGGCCCCTGCTACCCTCACCATCTTGC
  ACCAGCAGACAGAGGCAGTGCTGGGAGAGTGTCGGGTGCGTTTCCTCTCCTTCCTGGCCGTGGGCAGAGA
  TGTCCACACGTTTGCATTCATCATGGCTGCCGGCCCAGCCTCCTTCTGCTGCCACATGTTCTGGTGCGAG
  CCCAATGCTGCCAGCCTCTCAGAGGCTGTGCAGGCTGCGTGCATGCTTCGCTACCAGAAGTGTCTGGATG
  CCCGTTCCCAGGCCTCCACCTCCTGCCTCCCAGCACCCCCTGCTGAGTCTGTGGCACGGCGTGTAGGGTG
  GACTGTCCGCAGGGGTGTTCAGTCGCTGTGGGGCTCCCTGAAGCCCAAACGGCTGGGGGCCCATACCCCA
  TGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001257319
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001257319.2, NP_001244248.1
• RefSeq Size: 2026 bp
• RefSeq ORF: 1473 bp
• Locus ID: 322
• Cytogenetics: 11p15.4
• Protein Families: Transcription Factors
• Protein Pathways: Alzheimer's disease
• Gene Summary: 'The protein encoded by this gene is a member of the Fe65 protein family. It is an adaptor protein localized in the nucleus. It interacts with the Alzheimer's disease amyloid precursor protein (APP), transcription factor CP2/LSF/LBP1 and the low-density lipoprotein receptor-related protein. APP functions as a cytosolic anchoring site that can prevent the gene product's nuclear translocation. This encoded protein could play an important role in the pathogenesis of Alzheimer's disease. It is thought to regulate transcription. Also it is observed to block cell cycle progression by downregulating thymidylate synthase expression. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Mar 2012]'
  Transcript Variant: This variant (9) represents use of an alternate promoter and thus differs in the 5' UTR and 5' coding region compared to variant 1. These differences cause translation initiation at an alternate start codon and result in an isoform (g) with a shorter and distinct N-terminus, compared to isoform 1.