FLI1 (NM_001271012) Human Untagged Clone

CAT#: SC330911

FLI1 (untagged) - Homo sapiens Fli-1 proto-oncogene, ETS transcription factor (FLI1), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FLI1
• Synonyms: BDPLT21; EWSR2; SIC-1
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001271012, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGATCCAGGTTCACTGCTGGCCTATAATACAACCTCCCACACCGACCAATCCTCACGATTGAGTGTCA
  AAGAAGACCCTTCTTATGACTCAGTCAGAAGAGGAGCTTGGGGCAATAACATGAATTCTGGCCTCAACAA
  AAGTCCTCCCCTTGGAGGGGCACAAACGATCAGTAAGAATACAGAGCAACGGCCCCAGCCAGATCCGTAT
  CAGATCCTGGGCCCGACCAGCAGTCGCCTAGCCAACCCTGGAAGCGGGCAGATCCAGCTGTGGCAATTCC
  TCCTGGAGCTGCTCTCCGACAGCGCCAACGCCAGCTGTATCACCTGGGAGGGGACCAACGGGGAGTTCAA
  AATGACGGACCCCGATGAGGTGGCCAGGCGCTGGGGCGAGCGGAAAAGCAAGCCCAACATGAATTACGAC
  AAGCTGAGCCGGGCCCTCCGTTATTACTATGATAAAAACATTATGACCAAAGTGCACGGCAAAAGATATG
  CTTACAAATTTGACTTCCACGGCATTGCCCAGGCTCTGCAGCCACATCCGACCGAGTCGTCCATGTACAA
  GTACCCTTCTGACATCTCCTACATGCCTTCCTACCATGCCCACCAGCAGAAGGTGAACTTTGTCCCTCCC
  CATCCATCCTCCATGCCTGTCACTTCCTCCAGCTTCTTTGGAGCCGCATCACAATACTGGACCTCCCCCA
  CGGGGGGAATCTACCCCAACCCCAACGTCCCCCGCCATCCTAACACCCACGTGCCTTCACACTTAGGCAG
  CTACTACTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001271012
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001271012.1, NP_001257941.1
• RefSeq Size: 3442 bp
• RefSeq ORF: 780 bp
• Locus ID: 2313
• Cytogenetics: 11q24.3
• Protein Families: Transcription Factors
• Gene Summary: 'This gene encodes a transcription factor containing an ETS DNA-binding domain. The gene can undergo a t(11;22)(q24;q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. An acute lymphoblastic leukemia-associated t(4;11)(q21;q23) translocation involving this gene has also been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]'
  Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region and two alternate internal exons, and uses an alternate start codon, compared to variant 1. The encoded isoform (4) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.