RIT2 (NM_001272077) Human Untagged Clone

CAT#: SC331034

RIT2 (untagged) - Homo sapiens Ras-like without CAAX 2 (RIT2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RIT2
• Synonyms: RIBA; RIN; ROC2
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001272077, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGGTAGAAAATGAAGCCAGCTGCTCCCCGGGCAGCGCATCAGGCGGGTCCAGAGAGTACAAGGTGG
  TAATGCTGGGAGCAGGGGGAGTTGGTAAAAGCGCAATGACAATGCAGTTTATTAGTCATCAGTTCCCTGA
  TTATCATGACCCTACTATAGAAGATGCTTATAAGACCCAGGTCAGGATTGACAATGAGCCAGCTTACTTG
  GACATCTTGGACACTGCTGGCCAGGCAGAATTCACAGCCATGCGGGAGCAGTACATGCGAGGTGGGGAAG
  GCTTCATCATCTGCTACTCCGTCACTGACCGTCAATCATTTCAGGAGGCTGCCAAGTTTAAAGAGCTCAT
  TTTTCAGGTCCGCCACACCTATGAAATTCCCCTGGTGCTGGTGGGTAACAAAATTGATCTGGAACAGTTC
  CGCCAGCCTACACTGAAAGAGTTTGGAAGGGAAGAATTCTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001272077
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001272077.1, NP_001259006.1
• RefSeq Size: 1166 bp
• RefSeq ORF: 462 bp
• Locus ID: 6014
• Cytogenetics: 18q12.3
• Gene Summary: 'RIN belongs to the RAS (HRAS; MIM 190020) superfamily of small GTPases (Shao et al., 1999 [PubMed 10545207]).[supplied by OMIM, Mar 2008]'
  Transcript Variant: This variant (2) contains an alternate internal exon in the 3' coding region which results in a frameshift and an early stop codon, compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus, compared to isoform 1.