PPP2R5A (NM_001199756) Human Untagged Clone

CAT#: SC331355

PPP2R5A (untagged) - Homo sapiens protein phosphatase 2, regulatory subunit B', alpha (PPP2R5A), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPP2R5A
• Synonyms: B56A; B56alpha; PR61A
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001199756, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGATAATGAATGCCACTTCAAATGAACAACAAGAGCTTTTCTGTCAGAAGTTGCAGCAGTGTTGTATAC
  TGTTTGATTTCATGGACTCTGTTTCAGACTTGAAGAGCAAAGAAATTAAAAGAGCAACACTGAATGAACT
  GGTTGAGTATGTTTCAACTAATCGTGGTGTAATTGTTGAATCAGCGTATTCTGATATAGTAAAAATGATC
  AGTGCTAACATCTTCCGTACACTTCCTCCAAGTGATAATCCAGATTTTGATCCAGAAGAGGATGAACCCA
  CGCTTGAGGCCTCTTGGCCTCACATACAGTTGGTATATGAATTCTTCTTGAGATTTTTGGAGAGCCCTGA
  TTTCCAGCCTAGCATTGCAAAACGATACATTGATCAGAAATTCGTACAACAGCTCCTGGAGCTTTTTGAT
  AGTGAAGATCCCAGAGAACGTGACTTCCTGAAGACTGTTCTGCACCGAATTTATGGGAAATTTCTTGGAT
  TAAGAGCATTCATCAGAAAACAAATTAACAACATTTTCCTCAGGTTTATATATGAAACAGAACATTTCAA
  TGGTGTTGCTGAACTTCTTGAAATATTAGGAAGTATTATCAATGGCTTTGCATTGCCACTGAAAGCAGAA
  CATAAACAATTTCTAATGAAGGTTCTTATTCCTATGCATACTGCAAAAGGATTAGCTTTGTTTCATGCTC
  AGCTAGCATATTGTGTTGTACAGTTCCTGGAGAAAGATACAACACTAACAGAGCCAGTGATCAGAGGACT
  GCTGAAATTTTGGCCAAAAACCTGCAGTCAGAAAGAGGTGATGTTTTTAGGAGAAATTGAAGAAATCTTA
  GATGTCATTGAACCAACACAGTTCAAAAAAATTGAAGAGCCACTTTTCAAGCAGATATCCAAGTGTGTAT
  CCAGTTCTCATTTTCAGGTTGCAGAAAGGGCATTGTACTTCTGGAATAACGAATATATTCTTAGTTTGAT
  TGAGGAGAACATTGATAAAATTCTGCCAATTATGTTTGCCAGTTTGTACAAAATTTCCAAAGAACACTGG
  AATCCGACCATTGTAGCACTGGTATACAATGTGCTGAAAACCCTAATGGAAATGAATGGCAAGCTTTTCG
  ATGACCTTACTAGCTCATACAAAGCTGAAAGACAGAGAGAGAAAAAGAAGGAATTGGAACGTGAAGAATT
  ATGGAAAAAATTAGAGGAGCTAAAGCTAAAGAAAGCTCTAGAAAAACAGAATAGTGCTTACAACATGCAC
  AGTATTCTCAGCAATACAAGTGCCGAATAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001199756
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001199756.1, NP_001186685.1
• RefSeq Size: 2667 bp
• RefSeq ORF: 1290 bp
• Locus ID: 5525
• Cytogenetics: 1q32.3
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Oocyte meiosis, Wnt signaling pathway
• Gene Summary: 'The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an alpha isoform of the regulatory subunit B56 subfamily. Alternative transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Dec 2010]'
  Transcript Variant: This variant (2) has an alternate 5' exon, as compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus, as compared to isoform 1.