NOS1 (NM_001204218) Human Untagged Clone

CAT#: SC331527

NOS1 (untagged) - Homo sapiens nitric oxide synthase 1 (neuronal) (NOS1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NOS1
• Synonyms: bNOS; IHPS1; N-NOS; NC-NOS; nNOS; NOS
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001204218, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGGATCACATGTTCGGTGTTCAGCAAATCCAGCCCAATGTCATTTCTGTTCGTCTCTTCAAGCGCA
  AAGTTGGGGGCCTGGGATTTCTGGTGAAGGAGCGGGTCAGTAAGCCGCCCGTGATCATCTCTGACCTGAT
  TCGTGGGGGCGCCGCAGAGCAGAGTGGCCTCATCCAGGCCGGAGACATCATTCTTGCGGTCAACGGCCGG
  CCCTTGGTGGACCTGAGCTATGACAGCGCCCTGGAGGTACTCAGAGGCATTGCCTCTGAGACCCACGTGG
  TCCTCATTCTGAGGGGCCCTGAAGGTTTCACCACGCACCTGGAGACCACCTTTACAGGTGATGGGACCCC
  CAAGACCATCCGGGTGACACAGCCCCTGGGTCCCCCCACCAAAGCCGTGGATCTGTCCCACCAGCCACCG
  GCCGGCAAAGAACAGCCCCTGGCAGTGGATGGGGCCTCGGGTCCCGGGAATGGGCCTCAGCATGCCTACG
  ATGATGGGCAGGAGGCTGGCTCACTCCCCCATGCCAACGGCCTGGCCCCCAGGCCCCCAGGCCAGGACCC
  CGCGAAGAAAGCAACCAGAGTCAGCCTCCAAGGCAGAGGGGAGAACAATGAACTGCTCAAGGAGATAGAG
  CCTGTGCTGAGCCTTCTCACCAGTGGGAGCAGAGGGGTCAAGGGAGGGGCACCTGCCAAGGCAGAGATGA
  AAGATATGGGAATCCAGGTGGACAGAGATTTGGACGGCAAGTCACACAAACCTCTGCCCCTCGGCGTGGA
  GAACGACCGAGTCTTCAATGACCTATGGGGGAAGGGCAATGTGCCTGTCGTCCTCAACAACCCATATTCA
  GAGAAGGAGCAGCCCCCCACCTCAGGAAAACAGTCCCCCACAAAGAATGGCAGCCCCTCCAAGTGTCCAC
  GCTTCCTCAAGGTCAAGAACTGGGAGACTGAGGTGGTTCTCACTGACACCCTCCACCTTAAGAGCACATT
  GGAAACGGGATGCACTGAGTACATCTGCATGGGCTCCATCATGCATCCTTCTCAGCATGCAAGGAGGCCT
  GAAGACGTCCGCACAAAAGGACAGCTCTTCCCTCTCGCCAAAGAGTTTATTGATCAATACTATTCATCAA
  TTAAAAGATTTGGCTCCAAAGCCCACATGGAAAGGCTGGAAGAGGTGAACAAAGAGATCGACACCACTAG
  CACTTACCAGCTCAAGGACACAGAGCTCATCTATGGGGCCAAGCACGCCTGGCGGAATGCCTCGCGCTGT
  GTGGGCAGGATCCAGTGGTCCAAGCTGCAGGTATTCGATGCCCGTGACTGCACCACGGCCCACGGGATGT
  TCAACTACATCTGTAACCATGTCAAGTATGCCACCAACAAAGGGAACCTCAGGTCTGCCATCACCATATT
  CCCCCAGAGGACAGACGGCAAGCACGACTTCCGAGTCTGGAACTCCCAGCTCATCCGCTACGCTGGCTAC
  AAGCAGCCTGACGGCTCCACCCTGGGGGACCCAGCCAATGTGCAGTTCACAGAGATATGCATACAGCAGG
  GCTGGAAACCGCCTAGAGGCCGCTTCGATGTCCTGCCGCTCCTGCTTCAGGCCAACGGCAATGACCCTGA
  GCTCTTCCAGATTCCTCCAGAGCTGGTGTTGGAAGTTCCCATCAGGCACCCCAAGTTTGAGTGGTTCAAG
  GACCTGGGGCTGAAGTGGTACGGCCTCCCCGCCGTGTCCAACATGCTCCTAGAGATTGGCGGCCTGGAGT
  TCAGCGCCTGTCCCTTCAGTGGCTGGTACATGGGCACAGAGATTGGTGTCCGCGACTACTGTGACAACTC
  CCGCTACAATATCCTGGAGGAAGTGGCCAAGAAGATGAACTTAGACATGAGGAAGACGTCCTCCCTGTGG
  AAGGACCAGGCGCTGGTGGAGATCAATATCGCGGTTCTCTATAGCTTCCAGAGTGACAAAGTGACCATTG
  TTGACCATCACTCCGCCACCGAGTCCTTCATTAAGCACATGGAGAATGAGTACCGCTGCCGGGGGGGCTG
  CCCTGCCGACTGGGTGTGGATCGTGCCCCCCATGTCCGGAAGCATCACCCCTGTGTTCCACCAGGAGATG
  CTCAACTACCGGCTCACCCCCTCCTTCGAATACCAGCCTGATCCCTGGAACACGCATGTCTGGAAAGGCA
  CCAACGGGACCCCCACAAAGCGGCGAGCCATTGGCTTCAAGAAGCTAGCAGAAGCTGTCAAGTTCTCGGC
  CAAGCTGATGGGGCAGGCTATGGCCAAGAGGGTGAAAGCGACCATCCTCTATGCCACAGAGACAGGCAAA
  TCGCAAGCTTATGCCAAGACCTTGTGTGAGATCTTCAAACACGCCTTTGATGCCAAGGTGATGTCCATGG
  AAGAATATGACATTGTGCACCTGGAACATGAAACTCTGGTCCTTGTGGTCACCAGCACCTTTGGCAATGG
  AGATCCCCCTGAGAATGGGGAGAAATTCGGCTGTGCTTTGATGGAAATGAGGCACCCCAACTCTGTGCAG
  GAAGAAAGGAAGTACCCGGAACCCTTGCGTTTCTTTCCCCGTAAAGGGCCTCCCCTCCCCAATGGTGACA
  CAGAAGTCCACGGTCTGGCTGCAGCCCGTGACAGCCAGCACAGGAGCTACAAGGTCCGATTCAACAGCGT
  CTCCTCCTACTCTGACTCCCAAAAATCATCAGGCGATGGGCCCGACCTCAGAGACAACTTTGAGAGTGCT
  GGACCCCTGGCCAATGTGAGGTTCTCAGTTTTTGGCCTCGGCTCACGAGCATACCCTCACTTTTGCGCCT
  TCGGACACGCTGTGGACACCCTCCTGGAAGAACTGGGAGGGGAGAGGATCCTGAAGATGAGGGAAGGGGA
  TGAGCTCTGTGGGCAGGAAGAGGCTTTCAGGACCTGGGCCAAGAAGGTCTTCAAGGCAGCCTGTGATGTC
  TTCTGTGTGGGAGATGATGTCAACATTGAAAAGGCCAACAATTCCCTCATCAGCAATGATCGCAGCTGGA
  AGAGAAACAAGTTCCGCCTCACCTTTGTGGCCGAAGCTCCAGAACTCACACAAGGTCTATCCAATGTCCA
  CAAAAAGCGAGTCTCAGCTGCCCGGCTCCTTAGCCGTCAAAACCTCCAGAGCCCTAAATCCAGTCGGTCA
  ACTATCTTCGTGCGTCTCCACACCAACGGGAGCCAGGAGCTGCAGTACCAGCCTGGGGACCACCTGGGTG
  TCTTCCCTGGCAACCACGAGGACCTCGTGAATGCCCTGATCGAGCGGCTGGAGGACGCGCCGCCTGTCAA
  CCAGATGGTGAAAGTGGAACTGCTGGAGGAGCGGAACACGGCTTTAGGTGTCATCAGTAACTGGACAGAC
  GAGCTCCGCCTCCCGCCCTGCACCATCTTCCAGGCCTTCAAGTACTACCTGGACATCACCACGCCACCAA
  CGCCTCTGCAGCTGCAGCAGTTTGCCTCCCTAGCTACCAGCGAGAAGGAGAAGCAGCGTCTGCTGGTCCT
  CAGCAAGGGTTTGCAGGAGTACGAGGAATGGAAATGGGGCAAGAACCCCACCATCGTGGAGGTGCTGGAG
  GAGTTCCCATCTATCCAGATGCCGGCCACCCTGCTCCTGACCCAGCTGTCCCTGCTGCAGCCCCGCTACT
  ATTCCATCAGCTCCTCCCCAGACATGTACCCTGATGAAGTGCACCTCACTGTGGCCATCGTTTCCTACCG
  CACTCGAGATGGAGAAGGACCAATTCACCACGGCGTATGCTCCTCCTGGCTCAACCGGATACAGGCTGAC
  GAACTGGTCCCCTGTTTCGTGAGAGGAGCACCCAGCTTCCACCTGCCCCGGAACCCCCAAGTCCCCTGCA
  TCCTCGTTGGACCAGGCACCGGCATTGCCCCTTTCCGAAGCTTCTGGCAACAGCGGCAATTTGATATCCA
  ACACAAAGGAATGAACCCCTGCCCCATGGTCCTGGTCTTCGGGTGCCGGCAATCCAAGATAGATCATATC
  TACAGGGAAGAGACCCTGCAGGCCAAGAACAAGGGGGTCTTCAGAGAGCTGTACACGGCTTACTCCCGGG
  AGCCAGACAAACCAAAGAAGTACGTGCAGGACATCCTGCAGGAGCAGCTGGCGGAGTCTGTGTACCGAGC
  CCTGAAGGAGCAAGGGGGCCACATATACGTCTGTGGGGACGTCACCATGGCTGCTGATGTCCTCAAAGCC
  ATCCAGCGCATCATGACCCAGCAGGGGAAGCTCTCGGCAGAGGACGCCGGCGTATTCATCAGCCGGATGA
  GGGATGACAACCGATACCATGAGGATATTTTTGGAGTCACCCTGCGAACGTACGAAGTGACCAACCGCCT
  TAGATCTGAGTCCATTGCCTTCATTGAAGAGAGCAAAAAAGACACCGATGAGGTTTTCAGCTCCTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001204218
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001204218.1, NP_001191147.1
• RefSeq Size: 12291 bp
• RefSeq ORF: 4407 bp
• Locus ID: 4842
• Cytogenetics: 12q24.22
• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Arginine and proline metabolism, Calcium signaling pathway, Long-term depression, Metabolic pathways, Pathways in cancer, Small cell lung cancer
• Gene Summary: 'The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.[provided by RefSeq, Feb 2011]'
  Transcript Variant: This variant (2) contains an additional in-frame coding exon compared to variant 1, resulting in a longer isoform (2, also known as nNOSmu) containing a 34 aa protein segment not found in isoform 1. A similar isoform has also been reported in rat and mouse, and the mouse isoform has been shown to have catalytic activity (PMID:9791007). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.