PARN (NM_001242992) Human Untagged Clone

CAT#: SC331938

PARN (untagged) - Homo sapiens poly(A)-specific ribonuclease (PARN), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PARN
• Synonyms: DAN; DKCB6; PFBMFT4
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001242992, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGATAATCAGGAGCAATTTTAAGAGTAATCTTCACAAAGTGTACCAGGCCATAGAGGAGGCCGACT
  TCTTCGCCATCGATGGGGAGTTTTCAGGAATCAGTGATGGACCTTCAGTCTCTGCATTAACAAATGGTTT
  TGACACTCCAGAAGAGAGATCCTCACCAGATGTCAAATTTGTTTGTCAGAGCTCCAGCATTGACTTTCTA
  GCAAGCCAGGGATTTGATTTTAATAAAGTTTTTCGAAATGGAATTCCATATTTAAATCAGGAAGAAGAAA
  GACAGTTAAGAGAGCAGTATGATGAAAAACGTTCACAGGCGAATGGTGCAGGAGCTCTGTCCTATGTATC
  TCCTAACACTTCAAAATGTCCTGTCACGATTCCTGAGGATCAAAAGAAGTTTATTGACCAAGTGGTAGAG
  AAAATAGAGGATTTATTACAAAGTGAAGAAAACAAGAACTTGGATTTAGAGCCATGTACCGGGTTCCAAA
  GAAAACTAATTTATCAGACTTTGAGCTGGAAGTATCCGAAAGGCATTCATGTTGAGACTTTAGAAACTGA
  AAAGAAGGAGCGATATATAGTTATCAGCAAAGTAGATGAAGAAGAACGCAAAAGAAGAGAGCAGCAGAAA
  CATGCCAAAGAACAGGAGGAGCTGAATGATGCTGTGGGATTTTCTAGAGTCATTCACGCCATTGCTAATT
  CGGGAAAACTTGTTATTGGACACAATATGCTCTTGGACGTCATGCACACAGTTCATCAGTTCTACTGCCC
  TCTGCCTGCGGACTTAAGTGAGTTTAAAGAGATGACAACATGTGTTTTCCCCAGACTCTTGGATACTAAA
  TTGATGGCCAGCACACAACCTTTTAAGGATATCATTAACAACACATCCCTTGCGGAATTGGAAAAGCGGT
  TAAAAGAGACACCTTTCAACCCTCCTAAAGTTGAAAGTGCCGAAGGTTTTCCAAGTTATGACACAGCCTC
  TGAACAACTCCACGAGGCAGGCTACGATGCCTACATCACAGGGCTGTGCTTCATCTCCATGGCCAATTAC
  CTAGGTTCTTTTCTCAGCCCTCCAAAAATTCATGTGTCTGCCAGATCAAAACTCATTGAACCTTTTTTTA
  ACAAGTTATTTCTTATGAGGGTCATGGATATCCCCTATCTAAACTTGGAAGGACCAGACTTGCAGCCTAA
  ACGTGATCATGTTCTCCATGTGACATTCCCCAAAGAATGGAAAACCAGCGACCTTTACCAGCTTTTCAGT
  GCCTTTGGTAACATTCAGATATCCTGGATTGATGACACATCAGCATTTGTTTCCCTTAGCCAGCCCGAGC
  AAGTAAAGATTGCTGTCAATACCAGCAAATATGCAGAAAGCTATCGGATCCAAACCTATGCTGAATATAT
  GGGGAGAAAACAGGAAGAGAAGCAGATCAAAAGAAAGTGGACTGAAGATAGCTGGAAGGAGGCTGACAGC
  AAACGGTTAAACCCCCAGTGCATACCCTACACCCTGCAGAATCACTATTACCGCAACAATAGTTTTACAG
  CTCCCAGCACAGTAGGAAAGAGAAATTTGAGTCCTAGTCAAGAGGAAGCTGGCCTGGAGGACGGAGTGTC
  AGGGGAGATTTCCGACACTGAGCTTGAGCAGACCGATTCCTGTGCAGAGCCCCTCTCAGAGGGAAGGAAA
  AAGGCCAAGAAATTAAAAAGAATGAAGAAGGAGCTTTCTCCAGCAGGAAGCATCTCGAAGAACAGCCCTG
  CCACACTCTTTGAAGTTCCTGACACATGGTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001242992
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001242992.1, NP_001229921.1
• RefSeq Size: 2945 bp
• RefSeq ORF: 1782 bp
• Locus ID: 5073
• Cytogenetics: 16p13.12
• Protein Families: Transcription Factors
• Protein Pathways: RNA degradation
• Gene Summary: 'The protein encoded by this gene is a 3'-exoribonuclease, with similarity to the RNase D family of 3'-exonucleases. It prefers poly(A) as the substrate, hence, efficiently degrades poly(A) tails of mRNAs. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]'
  Transcript Variant: This variant (3) uses two alternate in-frame splice sites and lacks an alternate exon in the 5' coding region compared to variant 1. This results in a shorter protein (isoform 3), compared to isoform 1.