Myosin Phosphatase (PPP1R12A) (NM_001244992) Human Untagged Clone

CAT#: SC332112

PPP1R12A (untagged) - Homo sapiens protein phosphatase 1, regulatory subunit 12A (PPP1R12A), transcript variant 5

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPP1R12A
• Synonyms: M130; MBS; MYPT1
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001244992, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAAGATGGCGGACGCGAAGCAGAAGCGGAACGAGCAGCTGAAACGCTGGATCGGCTCCGAGACGGACC
  TCGAGCCTCCGGTGGTGAAGCGCCAGAAGACCAAGGTGAAGTTCGACGATGGCGCCGTCTTCCTGGCTGC
  TTGCTCCAGCGGCGACACGGACGAGGTCCTCAAGCTGCTGCACCGCGGCGCCGACATCAATTACGCCAAT
  GTGGACGGACTCACTGCCCTGCACCAGGCTTGCATTGATGACAATGTTGATATGGTGAAGTTTCTGGTAG
  AAAATGGAGCAAATATTAATCAACCTGATAATGAAGGCTGGATACCACTACATGCAGCAGCTTCCTGTGG
  ATATCTTGATATTGCAGAGTTTTTGATTGGTCAAGGAGCACATGTAGGGGCTGTCAACAGTGAAGGAGAT
  ACACCTTTAGATATTGCGGAGGAGGAGGCAATGGAAGAGCTACTTCAAAATGAAGTTAATCGGCAAGGGG
  TTGATATAGAAGCAGCTCGAAAGGAAGAAGAACGGATCATGCTTAGAGATGCCAGGCAGTGGCTAAATAG
  TGGTCATATAAATGATGTCCGGCATGCAAAATCTGGAGGTACAGCACTTCACGTTGCAGCTGCTAAAGGC
  TATACGGAAGTTTTAAAACTTTTAATACAGGCAGGCTATGATGTTAATATTAAAGACTATGATGGCTGGA
  CACCTCTTCATGCTGCAGCTCATTGGGGTAAAGAAGAAGCATGTCGAATTTTAGTGGACAATCTGTGTGA
  TATGGAGATGGTCAACAAAGTGGGCCAAACAGCCTTTGATGTAGCAGATGAAGACATTTTAGGATATTTA
  GAAGAGTTGCAAAAGAAACAAAATCTGCTCCATAGTGAAAAACGGGACAAGAAATCTCCACTAATTGAAT
  CAACAGCAAATATGGACAATAATCAGTCACAGAAGACCTTTAAAAACAAAGAGACGTTGATTATTGAACC
  AGAGAAAAATGCATCCCGTATTGAATCTCTGGAACAAGAAAAGGTTGATGAAGAAGAAGAAGGAAAGAAG
  GATGAGTCTAGCTGCTCTAGTGAAGAAGATGAGGAAGATGACTCGGAATCAGAAGCTGAAACAGATAAGA
  CAAAACCCCTGGCTTCTGTAACTAATGCCAACACTTCTAGTACACAAGCAGCTCCTGTAGCTGTTACAAC
  ACCTACTGTGTCATCAGGTCAAGCAACACCTACATCACCTATTAAAAAGTTTCCAACCACAGCTACAAAA
  ATTTCTCCCAAAGAAGAAGAGAGAAAAGATGAGTCTCCTGCAACTTGGAGGTTAGGACTTAGAAAGACGG
  GCAGCTATGGTGCACTTGCTGAAATCACAGCATCTAAAGAGGGTCAGAAAGAAAAAGATACTGCAGGTGT
  TACACGTTCAGCTTCAAGTCCCAGACTTTCCTCCTCTTTGGATAATAAAGAAAAGGAGAAAGATAGTAAA
  GGAACTAGGCTTGCATATGTTGCACCTACAATACCAAGACGACTAGCCAGTACATCTGACATTGAAGAGA
  AAGAAAACAGAGATTCTTCAAGTTTGCGAACAAGTAGTTCATATACAAGGAGAAAATGGGAAGATGATCT
  TAAAAAAAATAGCTCAGTTAATGAAGGATCAACGTATCATAAAAGTACCTCAAATCGTTTGTGGGCTGAG
  GATAGTACTGAGAAAGAAAAGGACAGTGTTCCTACGGCAGTGACCATTCCTGTTGCTCCAACTGTTGTAA
  ATGCTGCAGCTTCTACCACAACCCTGACTACAACTACTGCTGGCACTGTCTCCTCCACAACAGAGGTCAG
  GGAGAGACGCAGATCATACCTCACTCCTGTTAGGGATGAAGAGTCTGAATCCCAAAGAAAAGCAAGATCT
  AGACAAGCAAGACAATCTAGAAGATCAACACAGGGAGTGACATTAACTGATCTTCAAGAAGCTGAGAAAA
  CAATAGGAAGAAGTCGTTCTACCCGAACCAGAGAACAAGAAAATGAAGAAAAAGAAAAAGAGGAAAAAGA
  GAAACAAGATAAAGAGAAACAAGAAGAAAAGAAGGAGTCAGAAACATCTAGAGAAGATGAATATAAACAA
  AAGTACTCCAGAACGTATGATGAGACTTACCAGCGTTATAGGCCAGTATCAACTTCAAGTTCAACCACTC
  CATCCTCTTCACTTTCTACTATGAGCAGTTCACTGTATGCTTCAAGTCAACTAAACAGGCCAAATAGTCT
  TGTAGGCATAACTTCTGCTTACTCCAGAGGAATAACAAAAGAAAATGAAAGAGAGGGAGAAAAAAGAGAA
  GAGGAGAAAGAAGGAGAAGATAAATCACAACCTAAATCAATCAGAGAACGACGACGACCAAGAGAGAAAA
  GAAGATCTACAGGAGTTTCATTTTGGACACAAGATAGTGATGAAAATGAACAAGAACAACAATCAGACAC
  AGAAGAGGGATCCAATAAGAAAGAAACTCAGACGGATTCCATTTCTAGATATGAAACCAGTTCTACATCA
  GCTGGTGATCGATATGATTCCTTGCTGGGTCGCTCTGGATCATACAGTTACTTAGAAGAAAGAAAACCTT
  ACAGCAGCAGGCTAGAAAAGGATGACTCAACTGACTTTAAAAAGCTTTATGAACAAATTCTAGCTGAAAA
  TGAAAAGCTGAAGGCACAGCTACATGATACAAATATGGAACTAACAGATCTTAAATTACAGTTGGAAAAG
  GCCACCCAGAGACAAGAAAGATTTGCTGATAGATCACTGTTGGAAATGGAAAAAAGGGAACGAAGAGCTC
  TAGAAAGAAGAATATCTGAAATGGAAGAAGAGCTCAAAATGTTACCAGACCTAAAAGCAGACAACCAGAG
  GCTAAAGGATGAAAATGGGGCCTTGATCAGAGTTATAAGCAAACTTTCCAAATAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001244992
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001244992.1, NP_001231921.1
• RefSeq Size: 5311 bp
• RefSeq ORF: 2925 bp
• Locus ID: 4659
• Cytogenetics: 12q21.2-q21.31
• Protein Families: Druggable Genome
• Protein Pathways: Focal adhesion, Long-term potentiation, Regulation of actin cytoskeleton, Vascular smooth muscle contraction
• Gene Summary: 'Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2009]'
  Transcript Variant: This variant (5) lacks an exon in the coding region, compared to variant 1. The encoded isoform (d) is shorter than isoform a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.