GUCY1A2 (NM_001256424) Human Untagged Clone

CAT#: SC332380

GUCY1A2 (untagged) - Homo sapiens guanylate cyclase 1, soluble, alpha 2 (GUCY1A2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GUCY1A2
• Synonyms: GC-SA2; GUC1A2
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001256424, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCTCGAAGGAAGATTTCGTCCGAGTCCTTCAGCTCCCTGGGCTCCGACTACCTGGAGACCAGCCCGG
  AGGAGGAGGGGGAGTGCCCCCTGTCTAGGCTCTGCTGGAATGGCAGCCGGAGCCCGCCCGGGCCGCTGGA
  GCCCAGCCCGGCCGCAGCTGCCGCTGCCGCCGCCCCGGCCCCGACCCCGGCTGCTTCTGCCGCCGCCGCC
  GCTGCCACTGCCGGGGCCAGGAGGGTGCAGCGCCGGAGGCGGGTCAACCTGGACTCGCTGGGCGAGAGCA
  TCAGCCGCCTGACGGCGCCCTCGCCTCAGACGATACAGCAGACTCTCAAGAGGACACTGCAGTATTATGA
  ACATCAAGTTATTGGTTACAGGGATGCAGAAAAGAATTTCCACAATATCTCTAACAGATGCTCCTATGCA
  GACCACTCCAACAAAGAAGAAATTGAAGATGTCTCAGGAATTCTTCAGTGTACTGCTAATATACTCGGTT
  TGAAGTTTGAGGAAATTCAAAAAAGATTTGGTGAAGAGTTCTTTAATATATGCTTTCATGAGAATGAGAG
  AGTCCTTCGAGCTGTAGGTGGCACTTTGCAGGACTTTTTTAACGGCTTTGATGCTTTGTTGGAACACATT
  AGAACTTCTTTTGGAAAACAGGCCACTCTGGAGTCACCATCTTTCCTATGCAAAGAGCTCCCTGAAGGTA
  CTCTCATGCTCCACTACTTCCACCCTCACCATATTGTGGGGTTTGCAATGCTGGGGATGATTAAGGCTGC
  AGGAAAGAAGATCTATCGGCTGGATGTGGAAGTGGAACAGGTTGCAAATGAGAAGCTATGCTCTGATGTT
  TCAAACCCAGGCAATTGTAGCTGTCTTACTTTCCTTATCAAAGAATGTGAAAATACTAATATCATGAAGA
  ACCTTCCACAGGGAACCTCCCAAGTTCCTGCGGACCTCAGAATTAGCATCAACACCTTCTGTAGAGCCTT
  CCCTTTCCACTTGATGTTTGATCCCAGCATGTCAGTCCTTCAGTTGGGGGAAGGTCTAAGGAAGCAGCTT
  CGATGTGACACTCACAAAGTGCTCAAGTTTGAGGACTGCTTCGAGATTGTATCTCCAAAGGTTAATGCCA
  CCTTTGAAAGGGTCCTGCTGCGACTGTCTACCCCGTTTGTGATTAGAACCAAGCCTGAGGCTTCTGGCTC
  TGAAAATAAAGACAAGGTGATGGAAGTCAAAGGACAAATGATCCATGTTCCAGAATCAAATTCCATTTTA
  TTTTTGGGCTCTCCATGTGTGGACAAGTTGGATGAACTCATGGGCCGAGGGCTACATCTCTCAGACATCC
  CTATCCATGATGCCACCCGAGATGTCATTTTGGTTGGTGAGCAGGCAAAGGCCCAAGATGGGTTGAAGAA
  AAGGATGGATAAATTAAAGGCAACTTTAGAAAGAACTCACCAGGCCCTGGAAGAAGAGAAAAAGAAGACA
  GTGGATCTTCTATATTCTATTTTCCCTGGTGATGTAGCCCAGCAATTATGGCAAGGGCAGCAAGTACAGG
  CCAGAAAGTTTGATGATGTCACCATGCTCTTTTCAGACATTGTTGGCTTCACAGCCATATGTGCCCAGTG
  TACTCCCATGCAAGTAATCAGCATGCTGAATGAACTGTACACCAGATTTGACCACCAGTGTGGATTTTTG
  GATATTTATAAGGTGGAAACAATAGGTGATGCCTACTGTGTTGCAGCAGGGCTCCACAGAAAAAGCCTCT
  GCCATGCTAAACCCATTGCTCTGATGGCCTTGAAGATGATGGAACTTTCAGAAGAGGTGCTGACACCTGA
  TGGAAGACCGATTCAGCCCCAGAGGAGTGAACTCCTTTTCTCCTTTCCTGTTTCCATCCAGCTGGTTCCA
  GATCAGCATCAAAGTGAAACGGATTTGGGTACTGAAAAGATGAGGATAGGAATTCACTCAGGCTCCGTGC
  TGGCTGGAGTTGTTGGGGTGCGAATGCCACGTTATTGCCTGTTTGGAAATAATGTCACACTGGCAAGCAA
  ATTCGAGTCGGGAAGTCACCCTCGGCGCATCAATGTCAGCCCAACCACTTACCAATTATTAAAACGAGAA
  GAAAGTTTCACATTCATTCCGCGGTCTCGTGAAGAGCTTCCAGACAACTTTCCAAAGGAAATTCCTGGGA
  TCTGCTATTTCCTGGAGGTAAGGACTGGTCCAAAGCCACCAAAGCCTTCTCTTTCTTCGTCGAGAATAAA
  AAAGGTTTCCTACAACATCGGCACCATGTTCCTCCGGGAGACAAGCCTCTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001256424
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001256424.1, NP_001243353.1
• RefSeq Size: 16233 bp
• RefSeq ORF: 2292 bp
• Locus ID: 2977
• Cytogenetics: 11q22.3
• Protein Families: Druggable Genome
• Protein Pathways: Gap junction, Long-term depression, Purine metabolism, Vascular smooth muscle contraction
• Gene Summary: 'Soluble guanylate cyclases are heterodimeric proteins that catalyze the conversion of GTP to 3',5'-cyclic GMP and pyrophosphate. The protein encoded by this gene is an alpha subunit of this complex and it interacts with a beta subunit to form the guanylate cyclase enzyme, which is activated by nitric oxide. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.