NKCC1 (SLC12A2) (NM_001256461) Human Untagged Clone

CAT#: SC332389

SLC12A2 (untagged) - Homo sapiens solute carrier family 12 (sodium/potassium/chloride transporter), member 2 (SLC12A2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SLC12A2
• Synonyms: BSC; BSC2; NKCC1; PPP1R141
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001256461, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGCCGCGGCCCACGGCGCCCTCCTCCGGCGCCCCGGGACTGGCCGGGGTCGGGGAGACGCCGTCAG
  CCGCTGCGCTGGCCGCAGCCAGGGTGGAACTGCCCGGCACGGCTGTGCCCTCGGTGCCGGAGGATGCTGC
  GCCCGCGAGCCGGGACGGCGGCGGGGTCCGCGATGAGGGCCCCGCGGCGGCCGGGGACGGGCTGGGCAGA
  CCCTTGGGGCCCACCCCGAGCCAGAGCCGTTTCCAGGTGGACCTGGTTTCCGAGAACGCCGGGCGGGCCG
  CTGCTGCGGCGGCGGCGGCGGCGGCGGCAGCGGCGGCGGCTGGTGCTGGGGCGGGGGCCAAGCAGACCCC
  CGCGGACGGGGAAGCCAGCGGCGAGAGCGAGCCGGCTAAAGGCAGCGAGGAAGCCAAGGGCCGCTTCCGC
  GTGAACTTCGTGGACCCAGCTGCCTCCTCGTCGGCTGAAGACAGCCTGTCAGATGCTGCCGGGGTCGGAG
  TCGACGGGCCCAACGTGAGCTTCCAGAACGGCGGGGACACGGTGCTGAGCGAGGGCAGCAGCCTGCACTC
  CGGCGGCGGCGGCGGCAGTGGGCACCACCAGCACTACTATTATGATACCCACACCAACACCTACTACCTG
  CGCACCTTCGGCCACAACACCATGGACGCTGTGCCCAGGATCGATCACTACCGGCACACAGCCGCGCAGC
  TGGGCGAGAAGCTGCTCCGGCCTAGCCTGGCGGAGCTCCACGACGAGCTGGAAAAGGAACCTTTTGAGGA
  TGGCTTTGCAAATGGGGAAGAAAGTACTCCAACCAGAGATGCTGTGGTCACGTATACTGCAGAAAGTAAA
  GGAGTCGTGAAGTTTGGCTGGATCAAGGGTGTATTAGTACGTTGTATGTTAAACATTTGGGGTGTGATGC
  TTTTCATTAGATTGTCATGGATTGTGGGTCAAGCTGGAATAGGTCTATCAGTCCTTGTAATAATGATGGC
  CACTGTTGTGACAACTATCACAGGATTGTCTACTTCAGCAATAGCAACTAATGGATTTGTAAGAGGAGGA
  GGAGCATATTATTTAATATCTAGAAGTCTAGGGCCAGAATTTGGTGGTGCAATTGGTCTAATCTTCGCCT
  TTGCCAACGCTGTTGCAGTTGCTATGTATGTGGTTGGATTTGCAGAAACCGTGGTGGAGTTGCTTAAGGA
  ACATTCCATACTTATGATAGATGAAATCAATGATATCCGAATTATTGGAGCCATTACAGTCGTGATTCTT
  TTAGGTATCTCAGTAGCTGGAATGGAGTGGGAAGCAAAAGCTCAGATTGTTCTTTTGGTGATCCTACTTC
  TTGCTATTGGTGATTTCGTCATAGGAACATTTATCCCACTGGAGAGCAAGAAGCCAAAAGGGTTTTTTGG
  TTATAAATCTGAAATATTTAATGAGAACTTTGGGCCCGATTTTCGAGAGGAAGAGACTTTCTTTTCTGTA
  TTTGCCATCTTTTTTCCTGCTGCAACTGGTATTCTGGCTGGAGCAAATATCTCAGGTGATCTTGCAGATC
  CTCAGTCAGCCATACCCAAAGGAACACTCCTAGCCATTTTAATTACTACATTGGTTTACGTAGGAATTGC
  AGTATCTGTAGGTTCTTGTGTTGTTCGAGATGCCACTGGAAACGTTAATGACACTATCGTAACAGAGCTA
  ACAAACTGTACTTCTGCAGCCTGCAAATTAAACTTTGATTTTTCATCTTGTGAAAGCAGTCCTTGTTCCT
  ATGGCCTAATGAACAACTTCCAGGTAATGAGTATGGTGTCAGGATTTACACCACTAATTTCTGCAGGTAT
  ATTTTCAGCCACTCTTTCTTCAGCATTAGCATCCCTAGTGAGTGCTCCCAAAATATTTCAGGCTCTATGT
  AAGGACAACATCTACCCAGCTTTCCAGATGTTTGCTAAAGGTTATGGGAAAAATAATGAACCTCTTCGTG
  GCTACATCTTAACATTCTTAATTGCACTTGGATTCATCTTAATTGCTGAACTGAATGTTATTGCACCAAT
  TATCTCAAACTTCTTCCTTGCATCATATGCATTGATCAATTTTTCAGTATTCCATGCATCACTTGCAAAA
  TCTCCAGGATGGCGTCCTGCATTCAAATACTACAACATGTGGATATCACTTCTTGGAGCAATTCTTTGTT
  GCATAGTAATGTTCGTCATTAACTGGTGGGCTGCATTGCTAACATATGTGATAGTCCTTGGGCTGTATAT
  TTATGTTACCTACAAAAAACCAGATGTGAATTGGGGATCCTCTACACAAGCCCTGACTTACCTGAATGCA
  CTGCAGCATTCAATTCGTCTTTCTGGAGTGGAAGACCACGTGAAAAACTTTAGGCCACAGTGTCTTGTTA
  TGACAGGTGCTCCAAACTCACGTCCAGCTTTACTTCATCTTGTTCATGATTTCACAAAAAATGTTGGTTT
  GATGATCTGTGGCCATGTACATATGGGTCCTCGAAGACAAGCCATGAAAGAGATGTCCATCGATCAAGCC
  AAATATCAGCGATGGCTTATTAAGAACAAAATGAAGGCATTTTATGCTCCAGTACATGCAGATGACTTGA
  GAGAAGGTGCACAGTATTTGATGCAGGCTGCTGGTCTTGGTCGTATGAAGCCAAACACACTTGTCCTTGG
  ATTTAAGAAAGATTGGTTGCAAGCAGATATGAGGGATGTGGATATGTATATAAACTTATTTCATGATGCT
  TTTGACATACAATATGGAGTAGTGGTTATTCGCCTAAAAGAAGGTCTGGATATATCTCATCTTCAAGGAC
  AAGAAGAATTATTGTCATCACAAGAGAAATCTCCTGGCACCAAGGATGTGGTAGTAAGTGTGGAATATAG
  TAAAAAGTCCGATTTAGATACTTCCAAACCACTCAGTGAAAAACCAATTACACACAAAGAATCCAAAGGC
  CCTATTGTGCCTTTAAATGTAGCTGACCAAAAGCTTCTTGAAGCTAGTACACAGTTTCAGAAAAAACAAG
  GAAAGAATACTATTGATGTCTGGTGGCTTTTTGATGATGGAGGTTTGACCTTATTGATACCTTACCTTCT
  GACGACCAAGAAAAAATGGAAAGACTGTAAGATCAGAGTATTCATTGGTGGAAAGATAAACAGAATAGAC
  CATGACCGGAGAGCGATGGCTACTTTGCTTAGCAAGTTCCGGATAGACTTTTCTGATATCATGGTTCTAG
  GAGATATCAATACCAAACCAAAGAAAGAAAATATTATAGCTTTTGAGGAAATCATTGAGCCATACAGACT
  TCATGAAGATGATAAAGAGCAAGATATTGCAGATAAAATGAAAGAAGATGAACCATGGCGAATAACAGAT
  AATGAGCTTGAACTTTATAAGACCAAGACATACCGGCAGATCAGGTTAAATGAGTTATTAAAGGAACATT
  CAAGCACAGCTAATATTATTGTCATGAGTCTCCCAGTTGCACGAAAAGGTGCTGTGTCTAGTGCTCTCTA
  CATGGCATGGTTAGAAGCTCTATCTAAGGACCTACCACCAATCCTCCTAGTTCGTGGGAATCATCAGAGT
  GTCCTTACCTTCTATTCATAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001256461
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001256461.1, NP_001243390.1
• RefSeq Size: 6843 bp
• RefSeq ORF: 3591 bp
• Locus ID: 6558
• Cytogenetics: 5q23.3
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Vibrio cholerae infection
• Gene Summary: 'The protein encoded by this gene mediates sodium and chloride transport and reabsorption. The encoded protein is a membrane protein and is important in maintaining proper ionic balance and cell volume. This protein is phosphorylated in response to DNA damage. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]'
  Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.